Abstract: The present disclosure provides a method for propagating a crop using meristem excision, micropropagation, plantlet formation, plantlet singulation, plantlet transfer to artificial seeds, and planting artificial seeds. Methods are provided for producing and handling the plantlets and materials used to form artificial seeds. The artificial seeds may be used to store plantlets or may be planted to form a crop.

Abstract: Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

Abstract: Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

Abstract: Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Abstract: The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Abstract: The present disclosure relates to isolation and identification of unique Pseudomonas stutzeri strains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.

Abstract: Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Abstract: Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to Pseudomonas stutzeri and medium including an electron acceptor. The Pseudomonas stutzeri grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery.

Abstract: Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

Abstract: A method for in situ bioremediation of hydrocarbon-contaminated sites using an enriched steady state microbial consortium capable of modifying crude oil components under anaerobic denitrifying conditions is disclosed.

Abstract: The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Abstract: The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

Abstract: A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

Abstract: The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Abstract: The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Abstract: A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

Abstract: The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

Abstract: The present disclosure relates to isolation and identification of unique Pseudomonas stutzeri strains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.

Abstract: A process was developed for expressing antimicrobial peptides in a recombinant host cell that eliminates host cell toxicity and antimicrobial peptide degradation, as well as providing a process for rapid purification. Fusion proteins comprising a periplasmic targeting signal, cleavage site, and antimicrobial peptides provide the basis for this process which produces antimicrobial peptides that may be used in antimicrobial compositions and articles.

Abstract: The invention describes application of pure culture microbial strains under denitrifying conditions for growth on crude oil thereby modifying crude oil properties to enhance its recovery.