Patents by Inventor Shigeaki Nishii
Shigeaki Nishii has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9540678Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: GrantFiled: January 31, 2014Date of Patent: January 10, 2017Assignees: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Publication number: 20140141416Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: January 31, 2014Publication date: May 22, 2014Applicants: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.Inventors: Takeaki OZAWA, Naomi MISAWA, Kenji MIURA, Tasuku OKAMOTO, Shigeaki NISHII, Kenji MASUDA
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Patent number: 8552170Abstract: The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3?-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.Type: GrantFiled: June 4, 2010Date of Patent: October 8, 2013Assignee: Toyo Boseki Kabushiki KaishaInventors: Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami
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Patent number: 8470974Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: GrantFiled: May 28, 2010Date of Patent: June 25, 2013Assignees: The University of Tokyo, ProbeX Inc., Tokyo Boseki Kabushiki KaishaInventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Patent number: 8383797Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.Type: GrantFiled: November 13, 2006Date of Patent: February 26, 2013Assignees: Toyo Boseki Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Yoshihiro Ohmiya, Yoshihiro Nakajima, Vadim Viviani, Shigeaki Nishii, Tomomi Asai
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Publication number: 20120190824Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: May 28, 2010Publication date: July 26, 2012Applicants: THE UNIVERSITY OF TOKYO, TOYO BOSEKI KABUSHIKI KAISHA, PROBEX INC.Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Publication number: 20110281286Abstract: The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3?-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.Type: ApplicationFiled: June 4, 2010Publication date: November 17, 2011Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami
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Publication number: 20100112553Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.Type: ApplicationFiled: November 13, 2006Publication date: May 6, 2010Applicants: Toyo Boseki Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Yoshihiro OHMIYA, Yoshihiro NAKAJIMA, Vadim VIVIANI, Shigeaki NISHII, Tomomi ASAI
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Publication number: 20090220960Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.Type: ApplicationFiled: November 13, 2006Publication date: September 3, 2009Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Yoshihiro Ohmiya, Yoshihiro Nakajima, Vadim Viviani, Shigeaki Nishii, Tomomi Asai
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Patent number: 7211409Abstract: A compound of formula (1) and an immunoassay method for quantitative determination of dioxins in a sample using as a standard the compound of the following formula (1): wherein R1, R2, R3 and R4 may be the same or different and represent chlorine or hydrogen, n is an integer from 1 to 10, and Z represents an amino acid residue or peptide.Type: GrantFiled: August 18, 2003Date of Patent: May 1, 2007Assignees: Toyo Boseki Kabushiki Kaisha, Takuma Co., Ltd.Inventors: Shigeaki Nishii, Kazuhiro Matsui, Takuya Ishibashi, Masanori Oka, Hiroki Fujihira, Hirotsugu Mishima, Shizuo Kataoka
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Publication number: 20060166296Abstract: A compound of formula (1) and an immunoassay method for quantitative determination of dioxins in a sample using as a standard the compound of the following formula (1): wherein R1, R2, R3 and R4 may be the same or different and represent chlorine or hydrogen, n is an integer from 1 to 10, and Z represents an amino acid residue or peptide.Type: ApplicationFiled: August 18, 2003Publication date: July 27, 2006Inventors: Shigeaki Nishii, Kazuhiro Matsui, Takuya Ishibashi, Masanori Oka, Hiroki Fujihira, Hirotsugu Mishima, Shizuo Kataoka
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Publication number: 20040072260Abstract: The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require imobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.Type: ApplicationFiled: May 12, 2003Publication date: April 15, 2004Inventors: Yoshihiro Soya, Shigeaki Nishii, Kazuhiro Matsui, Takuya Ishibashi, Yoshihisa Kawamura
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Patent number: 6638725Abstract: The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require immobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.Type: GrantFiled: January 24, 2000Date of Patent: October 28, 2003Assignee: Toyo Boseki Kabushiki KaishaInventors: Yoshihiro Soya, Shigeaki Nishii, Kazuhiro Matsui, Takuya Ishibashi, Yoshihisa Kawamura
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Publication number: 20010046683Abstract: The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require immobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.Type: ApplicationFiled: January 24, 2000Publication date: November 29, 2001Inventors: Yoshihiro Soya, Shigeaki Nishii, Kazuhiro Matsui, Takuya Ishuibashi, Yoshihisa Kawamura