Patents by Inventor Shigeharu Fukuda
Shigeharu Fukuda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20100143389Abstract: An object of the present invention is to provide an immunomodulating agent in gut that can be ingested continuously in daily diet without adverse side effect. The object is attained by providing an immunomodulating agent in gut comprising a cyclic tetrasaccharide as an effective ingredient.Type: ApplicationFiled: September 15, 2006Publication date: June 10, 2010Inventors: Keiko Hino, Mayumi Kurose, Takeo Sakurai, Shinichiro Inoue, Tohru Ogawa, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
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Patent number: 7732426Abstract: The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide(s) or a saccharide composition comprising the same, and uses thereof. Cyclo{?6)-[?-D-Glcp-(1?4)]n-?-D-Glcp-(1?}??General Formula 1 (In General Formula 1, “n” means a number of 4 or 5).Type: GrantFiled: September 26, 2005Date of Patent: June 8, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Hikaru Watanabe, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7718404Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: GrantFiled: May 9, 2007Date of Patent: May 18, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20100120710Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: ApplicationFiled: April 23, 2008Publication date: May 13, 2010Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
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Patent number: 7709230Abstract: A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and ?-1,4 glucosidic linkage as a linkage at the non-reducing end, with an ?-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of ?-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, ?-1,6 glucosidic linkage as a linkage at the non-reducing end, and ?-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.Type: GrantFiled: July 12, 2007Date of Patent: May 4, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Tomoyuki Nishimoto, Takanobu Higashiyama, Hikaru Watanabe, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20100015671Abstract: An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-?-D-glucopyranosyl-(1>4)-?-D-glucopyranosyl-(1>6)-?-D-glucopyranosyl-(1>4)-?-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.Type: ApplicationFiled: November 16, 2007Publication date: January 21, 2010Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Kazuhisa Mukai, Hikaru Watanabe, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7592160Abstract: The present invention aims to provide a novel process for producing isomaltose and isomaltitol, and uses thereof, and it solves the object by establishing a process for producing isomaltose comprising a step of contacting a saccharide, having the ?-1,4 glucosidic linkage as the linkage of non-reducing end and a glucose polymerization degree of at least two, with an ?-isomaltosyl-transferring enzyme and an ?-isomaltosylglucosaccharide-forming enzyme derived from a specific microorganism; a process for producing isomaltitol using the isomaltose produced by the above process; saccharide compositions comprising the isomaltose and/or the isomaltitol produced by the above processes; and uses thereof.Type: GrantFiled: October 18, 2002Date of Patent: September 22, 2009Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Tomoyuki Nishimoto, Tomohiko Sonoda, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7582463Abstract: A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45° C. but below 60° C.; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology.Type: GrantFiled: November 29, 2006Date of Patent: September 1, 2009Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Takuo Yamamoto, Kazuhiko Maruta, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7575900Abstract: A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45° C. but below 60° C.; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology.Type: GrantFiled: November 29, 2006Date of Patent: August 18, 2009Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Takuo Yamamoto, Kazuhiko Maruta, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20090022872Abstract: An object of the present invention is to provide a novel starchy substance having a retrogradation-resistance, a process for producing the starchy substance efficiently from a material starch by enzymatic reaction, and uses thereof. The present invention attains the above object by providing branched starch having 6-?-maltosyl- and/or 6-?-maltotetraosyl-structure(s) with a marked retrogradation-resistance, a process for producing the branched starch without lowering the molecular weight of material starch, and uses thereof.Type: ApplicationFiled: March 14, 2006Publication date: January 22, 2009Inventors: Tomoyuki Nishimoto, Katsuhiko Hino, Takanori Okura, Hiroto Chaen, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20090023684Abstract: The present invention has an object to provide a fluid for peritoneal dialysis with satisfactory body fluid removability, high biocompatibility, and improved storage stability, and the object is attained by a fluid for peritoneal dialysis containing one or more saccharides selected from cyclonigerosylnigerose, cyclomaltosylmaltose, and L-ascorbic acid 2-glucoside.Type: ApplicationFiled: April 13, 2006Publication date: January 22, 2009Inventors: Hitomi Ohta, Toshiharu Hanaya, Shigeharu Fukuda, Yoshikatsu Miwa, Toshio Miyake
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Publication number: 20080241904Abstract: A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and ?-1,4 glucosidic linkage as a linkage at the non-reducing end, with an ?-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of ?-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, ?-1,6 glucosidic linkage as a linkage at the non-reducing end, and ?-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.Type: ApplicationFiled: July 12, 2007Publication date: October 2, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Michio Kubota, Tomoyuki Nishimoto, Takanobu Higashiyama, Hikaru Watanabe, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20080214499Abstract: The object of the present invention is to provide a lipid-regulating agent or a composition for regulating the amount of lipids comprising the agent. The present invention solves the above object by providing a lipid-regulating agent comprising a cyclic tetrasaccharide and/or its saccharide-derivative(s) and a composition for regulating the amount of lipids comprising the lipid-regulating agent.Type: ApplicationFiled: February 19, 2008Publication date: September 4, 2008Applicant: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Kazuyuki Oku, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20080131468Abstract: The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide (s) or a saccharide composition comprising the same, and uses thereof.Type: ApplicationFiled: September 26, 2005Publication date: June 5, 2008Inventors: Hikaru Watanabe, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7375095Abstract: For extending the uses of a compound represented by Chemical formula 1, the object of the present invention is to provide a derivative of cyclic tetrasaccharide whose physicochemical properties are changed from those of cyclic tetrasaccaride, a composition comprising the same, and a process for producing the same. The present invention solves the above object by providing a derivative of cyclic tetrasaccharide, which is produced by the steps of reacting a compound represented by Chemical formula 1 with a reactive reagent and substituting one ore more hydroxyl groups with substituents except hydroxyl group and O-glycosyl group; a composition comprising the same; and a process for producing the derivative of cyclic tetrasaccharide.Type: GrantFiled: November 19, 2002Date of Patent: May 20, 2008Assignee: Kabashiki Kaisha Hayashibara Seibutsu KagakuInventors: Kazuyuki Oku, Naoki Kudo, Shigeharu Fukuda
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Publication number: 20080113938Abstract: The present invention has an object to provide an accelerator for mineral absorption and a composition containing the accelerator. The object is solved by providing an accelerator for mineral absorption comprising cyclic tetrasaccharide and/or saccharide derivatives thereof and a composition containing the accelerator.Type: ApplicationFiled: December 20, 2007Publication date: May 15, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Kazuyuki OKU, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7355014Abstract: A human IFN-? inducing polypeptide and its nucleotide sequence was isolated, purified and characterized. Pharmaceutical compositions containing this novel INF-? inducing polypeptide or active fragments thereof are formulated and monoclonal antibodies are raised against this polypeptide or antigenic fragments thereof. The polypeptide can be used in a method for treating diseases susceptive to treatment with INF-?, and methods for enhancing the cytotoxicity of NK cells or for inducing the formation of LAK cells, such as to treat tumors and malignant pathologies.Type: GrantFiled: October 16, 2006Date of Patent: April 8, 2008Assignee: Kabushiki Kaisha Hay Ashibara Seibutsu Kagaku KenkyujoInventors: Shimpei Ushio, Kakuji Torigoe, Todao Tanimoto, Haruki Okamura, Masashi Kurimoto, Shigeharu Fukuda, Toshio Kunikata, Mutsuko Taniguchi, Keizo Kohno
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Publication number: 20080032350Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: ApplicationFiled: May 9, 2007Publication date: February 7, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
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Publication number: 20080003651Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: ApplicationFiled: May 9, 2007Publication date: January 3, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7309697Abstract: An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{?6)-?-D-glucopyranosyl-(1?4)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?4)-?-D-glucopyranosyl-(1?6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.Type: GrantFiled: August 26, 2004Date of Patent: December 18, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Kazuhisa Mukai, Hikaru Watanabe, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Toshio Miyake