Patents by Inventor Shigekazu Hokazono

Shigekazu Hokazono has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Polypeptides having DNA polymerase activity
    Patent number: 8344105
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: January 1, 2013
    Assignee: Takara Bio Inc.
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY
    Publication number: 20120083590
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Application
    Filed: September 22, 2011
    Publication date: April 5, 2012
    Applicant: Takara Bio Inc.
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Polypeptides having DNA polymerase activity
    Patent number: 8048987
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Grant
    Filed: February 18, 2010
    Date of Patent: November 1, 2011
    Assignee: Takara Bio Inc.
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Thermostable ribonuclease H from
    Patent number: 7932071
    Abstract: A polypeptide having an RNaseH activity from Archaeoglobus profundus and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: April 26, 2011
    Assignee: Takara Bio Inc.
    Inventors: Shigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
  • POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY
    Publication number: 20100143979
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Application
    Filed: February 18, 2010
    Publication date: June 10, 2010
    Applicant: Takara Bio Inc.
    Inventors: Yoshimi SATO, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Polypeptides having DNA polymerase activity
    Patent number: 7704713
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Grant
    Filed: May 12, 2005
    Date of Patent: April 27, 2010
    Assignee: Takara Bio Inc.
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • THERMOSTABLE RIBONUCLEASE H
    Publication number: 20090269809
    Abstract: A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
    Type: Application
    Filed: June 29, 2009
    Publication date: October 29, 2009
    Applicant: Takara Bio. Inc.
    Inventors: Shigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
  • Thermostable ribonuclease H obtained from
    Patent number: 7572617
    Abstract: An isolated polypeptide having a thermostable ribonuclease H activity from Archaeoglobus profundus is highly useful in genetic engineering, as well as a gene encoding this polypeptide and a genetic engineering process for producing the polypeptide.
    Type: Grant
    Filed: August 26, 2003
    Date of Patent: August 11, 2009
    Assignee: Takara Bio Inc.
    Inventors: Shigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
  • Polypeptides having dna polymerase activity
    Publication number: 20090098610
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Application
    Filed: May 12, 2005
    Publication date: April 16, 2009
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Thermostable ribonuclease h
    Publication number: 20070037247
    Abstract: A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding. this polypeptide; and a genetic engineering process for producing the polypeptide.
    Type: Application
    Filed: August 26, 2003
    Publication date: February 15, 2007
    Applicant: Takara Bio. Inc.
    Inventors: Shigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
  • Amplified nucleic acids and immobilized products thereof
    Publication number: 20050118578
    Abstract: A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.
    Type: Application
    Filed: February 6, 2002
    Publication date: June 2, 2005
    Applicant: TAKARA BIO INC.
    Inventors: Junichi Mineno, Osamu Takeda, Masatomo Rokushima, Takashi Uemori, Shigekazu Hokazono, Hiroyuki Mukai, Shusaku Yamashita, Hiroaki Sagawa, Kiyozo Asada, Ikunoshin Kato
  • Method of determining nucleic acid base sequence
    Publication number: 20040203008
    Abstract: A method of determining the base sequence of a nucleic acid characterized by involving: the step of amplifying a template nucleic acid in the presence of at least two primers each having a tag sequence, a primer specific to the template nucleic acid and a DNA polymerase, wherein the primers having tag sequences have each the tag sequence in the 5′-terminal side thereof and a specific base sequence consisting of three or more nucleotides in the 3′-terminal side; and the step of directly sequencing the amplified fragments obtained in the above step.
    Type: Application
    Filed: April 30, 2003
    Publication date: October 14, 2004
    Inventors: Takashi Uemori, Hiroshige Yamashita, Shigekazu Hokazono, Yoshimi Sato, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato