Abstract: The purpose of the present invention is to provide a method of purification and preparation of cultured corneal endothelial cells, and in particular, to provide cell surface markers for use in corneal endothelial cells not including transformed cells. Provided are cell markers for distinguishing normal cells and transformed cells, in particular normal and transformed corneal endothelium cells. These cell markers relate to specific cell surface markers, for example, to a normal corneal endothelial surface marker such as CD166, and a transformed cell surface marker such as CD73. By using the transformed cell surface marker such as CD73 to remove transformed cells by sorting, it becomes possible to improve purity of a normal cultured corneal endothelium. By using normal corneal endothelial surface marker such as CD166, or by combined use with the transformed cell surface marker, it becomes possible to provide a means for verifying the purity of a prepared corneal endothelium.

Abstract: The present invention provides an agent for treating or preventing a disease, a disorder or a condition of a corneal endothelium, said agent comprising a corneal endothelial cell and a myosin II-specific inhibitor. More specifically, the myosin II-specific inhibitor is blebbistatin. The disease, the disorder or the condition is typically a disorder associated with Fuchs endothelial corneal dystrophy or corneal endothelial dysfunction (bullous keratopathy). Also provided is a method for treating or preventing a corneal endothelial disease, said method comprising a step of administering an effective amount of a myosin II-specific inhibitor together with a corneal endothelial cell to a subject.

Abstract: A substrate processing device according to the present embodiment includes a processing tank configured to be capable of accumulating a liquid. A conveyer can array a plurality of semiconductor substrates in such a manner that front surfaces of the semiconductor substrates face a substantially horizontal direction, and transport the semiconductor substrates into the processing tank. A plurality of liquid suppliers can supply the liquid toward an inside of the processing tank from a lower portion of the processing tank. A plurality of current plates are arranged on at least either one end side or the other end side of an array of the semiconductor substrates. The current plates are provided in a first gap region above the semiconductor substrates in gaps between the conveyer and a sidewall of the processing tank on both sides of the conveyer as viewed from an array direction of the semiconductor substrates.

Abstract: The inventors have found that VCP inhibitors protect corneal endothelial cells and corneal epithelial cells. Accordingly, the disclosure provides a composition for protecting a cornea comprising a compound of formula (I) or an ester, oxide, pharmaceutically acceptable salt or solvate thereof. In another aspect the disclosure provides a composition for treating and/or preventing a corneal disease comprising a compound of formula (I). In a further aspect the disclosure provides a composition for ocular irrigation comprising a compound of formula (I). In a still further aspect the disclosure provides a composition for preserving a corneal graft comprising a compound of formula (I).

Abstract: The present invention complete a technique of treating a corneal disorder or disease by infusion into an anterior chamber of human eyes. Specifically, the present invention based on the findings discovered that cultured human corneal endothelial cells are comprised of a plurality of subpopulations, most of them are not suitable for infusion into patients. The above-described subject was overcome by providing, as a medicament, functionally high grade quality of cells having the function of mature differentiated human corneal endothelial cells which is a specific subpopulation and characterized by their biochemical and functional phenotypes. The present invention provides such a functional mature differentiated corneal endothelial cells, medicament comprising the same, and manufacturing method, quality control and techniques related thereto.

Abstract: The purpose of the present invention is to provide a method of purification and preparation of cultured corneal endothelial cells, and in particular, to provide cell surface markers for use in corneal endothelial cells not including transformed cells. Provided are cell markers for distinguishing normal cells and transformed cells, in particular normal and transformed corneal endothelium cells. These cell markers relate to specific cell surface markers, for example, to a normal corneal endothelial surface marker such as CD166, and a transformed cell surface marker such as CD73. By using the transformed cell surface marker such as CD73 to remove transformed cells by sorting, it becomes possible to improve purity of a normal cultured corneal endothelium. By using normal corneal endothelial surface marker such as CD166, or by combined use with the transformed cell surface marker, it becomes possible to provide a means for verifying the purity of a prepared corneal endothelium.

Abstract: Provided is an agent for treating or preventing a corneal endothelial disorder wherein cell proliferation is required. More specifically, provided is an agent for treating or preventing a corneal endothelial disorder, wherein cell proliferation is required, said agent comprising a p38MAP kinase inhibitor. In a preferred embodiment, the corneal endothelial disorder is a wound. In a preferred embodiment, the p38MAP kinase inhibitor is soluble in water. The p38MAP kinase inhibitor may comprise 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole (SB203580) or a salt thereof.

Abstract: A cell evaluation method evaluates the quality of a cell population including a plurality of cells. The cell evaluation method comprises: an index calculation step of calculating an index, based on a captured image of the cell population, the index including at least any one of an average distance representing a packing degree of the cells, a spring constant representing a degree of consistency in distances between the cells, and a hexagonal order parameter representing a degree to which an arrangement of the cells resembles a regular hexagon; and an evaluation step of evaluating the cell population, based on the index calculated in the index calculation step.

Abstract: According to one embodiment, a semiconductor device includes a substrate, a stacked body, and a columnar portion. The stacked body, provided on the substrate, includes first conductive layers and first insulating layers provided alternately along a first direction. The columnar portion extends through the stacked body in the first direction. The columnar portion includes a blocking layer, a charge storage layer, a tunneling layer, and a semiconductor layer. The columnar portion includes a first portion and a second portion. The second portion is provided on the substrate side of the first portion. A dimension in the second direction of the second portion is smaller than a dimension in a second direction of the first portion. A portion of the blocking layer is provided at the second portion being thicker than a portion of the blocking layer provided at the first portion.

Abstract: According to one embodiment, a semiconductor memory device includes a stacked body, first memory portions, and second memory portions. The stacked body includes conductive layers. The conductive layers are arranged in a first direction and extend in a second direction. The stacked body includes first and second regions. The second region is arranged with the first region in the second direction. The first memory portions extend in the first direction through the first region and are arranged at a first pitch along the second direction. The second memory portions extend in the first direction through the second region and are arranged at the first pitch along the second direction. A distance between a first center of one of the first memory portions and a second center of one of the second memory portions is longer than the first pitch and shorter than 2 times the first pitch.

Abstract: A method for producing refined-coal has steps of (A) heating a mixture including moisture-containing coal and oil, to dehydrate the coal; (B) removing, in a first mist separator having plural baffle plates disposed in parallel, fine particles accompanying vapor generated by the dehydration step and adhered to the surface of the a first mist separator by spraying a liquid at a temperature of 50 ° C. or above and 150 ° C. or below in the first mist separator, which temperature is lower than a temperature to which the mixture is heated in step (A), wherein the sprayed liquid includes an oil component; and (C) compressing the vapor to obtain high-temperature vapor. In the step (B), the vapor is made to sequentially pass through the first mist separator and a second mist separator of a mesh type, while spraying liquid onto the first mist separator through which the vapor passes firstly.

Abstract: According to one embodiment, a semiconductor memory device includes a substrate, a stacked body, columnar parts, and insulating members. The stacked body is provided above the substrate, and includes electrode films stacked to be separated one another. The columnar parts are provided in the stacked body, and each of the columnar parts includes a semiconductor part. The insulating members are provided in the stacked body, and each of the insulating members includes insulating portions disposed alternately with the columnar parts. The insulating members include first, second, and third insulating members. The first and second insulating members are disposed to be separated each other. The third insulating member is positioned to be separated from the first and second insulating members. The insulating portions include a first insulating portion and second insulating portions. The columnar parts include first, second, third and fourth columnar parts.

Abstract: A semiconductor device includes first and second memory cells, a first word line, and a first and second bit lines, and a row control circuit. The first memory cell has a first gate electrode and a first channel having one end and another end. The second memory cell has a second gate electrode and a second channel having one end and another end. The first word line electrically connected with each of the first gate electrode and the second gate electrode. The first and second bit lines electrically connected with the first and second channels, respectively. When a threshold voltage of each of the first and second memory cells are caused to be shifted, the semiconductor device causes a first voltage between the first gate electrode and the first channel and a second voltage between the second gate electrode and the second channel to be differentiated.

Abstract: Provided is a method that produces a solid fuel having a relatively high strength from a powder fuel. The method includes: blending a coal-derived powder fuel with a pulverized fuel having a greater mean particle diameter than the coal-derived powder fuel to obtain a mixture; compression-molding the mixture to obtain a solid fuel; and pulverizing a part of the solid fuel, in which the part of the pulverized solid fuel is used as the pulverized fuel in the blending. A blending proportion of the pulverized fuel with respect to the blending mixture is preferably at least 5 mass % and at most 50 mass %. A cohesive fine coal having a superior cohesive property to the coal-derived powder fuel is preferably further blended in the blending. A blending proportion of the cohesive fine coal with respect to the blending mixture is preferably at least 5 mass % and at most 30 mass %.

Abstract: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate. [Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-? receptor inhibitor in various combinations.

Abstract: A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.

Abstract: The present invention complete a technique of treating a corneal disorder or disease by infusion into an anterior chamber of human eyes. Specifically, the present invention based on the findings discovered that cultured human corneal endothelial cells are comprised of a plurality of subpopulations, most of them are not suitable for infusion into patients. The above-described subject was overcome by providing, as a medicament, functionally high grade quality of cells having the function of mature differentiated human corneal endothelial cells which is a specific subpopulation and characterized by their biochemical and functional phenotypes. The present invention provides such a functional mature differentiated corneal endothelial cells, medicament comprising the same, and manufacturing method, quality control and techniques related thereto.

Abstract: [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.

Abstract: The present invention is provided with a step for preheating coal, a step for heating an extraction solvent, a step for mixing the preheated coal and the extraction solvent heated to a higher temperature than the preheated coal and thereby heating the coal, a step for separating a solution in which a coal component is dissolved from the mixture of the coal and the extraction solvent, and a step for evaporating and separating the extraction solvent from the solution.

Abstract: A method for producing ash-free coal includes: pulverizing a coal in the presence of a protection solvent; heating an extraction solvent; mixing pulverized matter obtained after the pulverizing, with the extraction solvent obtained after the heating; separating a solution containing a coal component dissolved therein, from a slurry obtained after the mixing; and evaporatively separating the protection solvent and the extraction solvent from the solution obtained after the separating.