Patents by Inventor Shih-hui Chao
Shih-hui Chao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10471426Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: GrantFiled: July 13, 2018Date of Patent: November 12, 2019Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Patent number: 10260090Abstract: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.Type: GrantFiled: July 18, 2016Date of Patent: April 16, 2019Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Rhett L. Martineau, Shih-hui Chao, Weimin Gao, Shufang Ci, Deirdre R. Meldrum
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Patent number: 10162162Abstract: Microfluidic devices for 3D hydrodynamic microvortical rotation of at least one live single cell or cell cluster, systems incorporating the devices, and methods of fabricating and using the devices and systems, are provided. A microfluidic chip rotates at least one live single cell or cell cluster in a microvortex about a stable rotation axis perpendicular to an optical axis within a chamber having a trapezoidal cross-sectional shape located below a flow channel. An optical trap may be used to position the cell or cells with the microvortex, and the cell or cells may be subject to live-cell or cell cluster computer tomography imaging.Type: GrantFiled: September 22, 2015Date of Patent: December 25, 2018Assignee: ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA, ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Hong Wang, Deirdre Meldrum, Rishabh Shetty, Laimonas Kelbauskas, Shih-Hui Chao, Roger Johnson, Jakrey Myers, Samantha Chan
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Publication number: 20180318835Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: ApplicationFiled: July 13, 2018Publication date: November 8, 2018Inventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Patent number: 10022718Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: GrantFiled: October 26, 2015Date of Patent: July 17, 2018Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Publication number: 20170107549Abstract: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.Type: ApplicationFiled: July 18, 2016Publication date: April 20, 2017Applicant: Arizona Board of Regents on behalf of Arizona State UniversityInventors: Rhett L. Martineau, Shih-hui Chao, Weimin Gao, Shufang Ci, Deirdre R. Meldrum
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Publication number: 20160114323Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.Type: ApplicationFiled: October 26, 2015Publication date: April 28, 2016Inventors: Rhett Martineau, Jeff Houkal, Shih-Hui Chao, Weimin Gao, Shufang Ci, Deirdre Meldrum
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Publication number: 20160084750Abstract: Microfluidic devices for 3D hydrodynamic microvortical rotation of at least one live single cell or cell cluster, systems incorporating the devices, and methods of fabricating and using the devices and systems, are provided. A microfluidic chip rotates at least one live single cell or cell cluster in a microvortex about a stable rotation axis perpendicular to an optical axis within a chamber having a trapezoidal cross-sectional shape located below a flow channel. An optical trap may be used to position the cell or cells with the microvortex, and the cell or cells may be subject to live-cell or cell cluster computer tomography imaging.Type: ApplicationFiled: September 22, 2015Publication date: March 24, 2016Inventors: Hong Wang, Deirdre Meldrum, Rishabh Shetty, Laimonas Kelbauskas, Shih-Hui Chao, Roger Johnson, Jakrey Myers, Samantha Chan
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Publication number: 20120231533Abstract: A chamber device for analyzing living cell(s). The chamber device includes a base and a lid that when reversibly pressed closed create a chamber. The base is configured with an optically transparent well to contain at least one cell. The lid has a breadth greater than the base and is configured to contain at least one sensor. The lid is further configured with a lip that when pressed between the lid and the base creates an impermeable seal. The base and the lid are configured so that, when closed and in use, the sensor remains spatially apart from the at least one cell.Type: ApplicationFiled: October 28, 2009Publication date: September 13, 2012Inventors: Mark R. Holl, Deirdre R. Meldrum, A. Cody Youngbull, Haixin Zhu, Jeff Houkal, Yanqing Tian, Shashanka Ashili, Laimonas Kelbauskas, Roger Johnson, Shih-hui Chao, Peter Wiktor, Alex Jen, Lloyd Burgess, Sarah McQuaid, Ai Brunner, Peter Kahn