Patents by Inventor Shujiro Sakaki

Shujiro Sakaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8278097
    Abstract: The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies.
    Type: Grant
    Filed: June 8, 2009
    Date of Patent: October 2, 2012
    Assignee: NOF Corporation
    Inventors: Hiroshi Kurosawa, Shujiro Sakaki
  • Patent number: 8252531
    Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.
    Type: Grant
    Filed: April 25, 2002
    Date of Patent: August 28, 2012
    Assignee: NOF Corporation
    Inventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
  • Patent number: 7648833
    Abstract: The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies.
    Type: Grant
    Filed: June 25, 2004
    Date of Patent: January 19, 2010
    Assignee: NOF Corporation
    Inventors: Hiroshi Kurosawa, Shujiro Sakaki
  • Publication number: 20090246871
    Abstract: The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies.
    Type: Application
    Filed: June 8, 2009
    Publication date: October 1, 2009
    Applicant: NOF CORPORATION
    Inventors: Hiroshi KUROSAWA, Shujiro SAKAKI
  • Patent number: 7166476
    Abstract: The present invention provides an agglutination immunoassay, wherein the agglutination of insoluble carrier particles such as latex are stabilized and uniformized to give good reproducibility, and a reagent therefor. In the agglutination immunoassay which comprises allowing an antigenic substance in a sample to bind to insoluble carrier particles carrying substantially neither antigens nor antibodies thereon, and allowing an antibody or an antibody complex which reacts specifically to the antigenic substance to bind to the antigenic substance to give a selective agglutination of the insoluble carrier particles, a homopolymer prepared by polymerization of a monomer such as 2-methacryloyloxyethyl phosphorylcholine having a phosphorylcholine group and a vinyl group, or a copolymer prepared by polymerization of a monomer having a phosphorylcholine group and a vinyl group, with a monomer having a vinyl group such as n-butyl methacrylate is used.
    Type: Grant
    Filed: August 28, 2001
    Date of Patent: January 23, 2007
    Assignees: Kyowa Medex Co., Ltd., NOF Corporation
    Inventors: Kayoko Shigenobu, Kenshiro Shuto, Shujiro Sakaki
  • Publication number: 20060252148
    Abstract: The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a coating layer formed from a compound having a particular PC-like group on a vessel surface defining a region for floating culture of ES cells, and (B) floating culturing ES cells in the vessel to form embryoid bodies.
    Type: Application
    Filed: June 25, 2004
    Publication date: November 9, 2006
    Inventors: Hiroshi Kurosawa, Shujiro Sakaki
  • Patent number: 6858673
    Abstract: A composition for hydrogels which comprises (A1) a copolymer which comprises 25 to 97 mol % of unit (a) derived from a compound represented by formula (1) and 3 to 75 mol % of unit (b) derived from a monomer represented by formula (2) and is soluble in aqueous media and (B) a polymer which has hydrophilic groups such as hydroxyl or carboxyl groups and is soluble in aqueous media; and a hydrogel comprising the composition. (X represents a divalent organic residue; Y represents alkyleneoxy, etc.; Z, R1, R2, R3, R4, and L2 each represents hydrogen, etc.; and L1 represents —C6H4—, etc.
    Type: Grant
    Filed: May 9, 2000
    Date of Patent: February 22, 2005
    Assignee: NOF Corporation
    Inventors: Nobuyuki Sakamoto, Kenshiro Shuto, Shujiro Sakaki, Ken Suzuki, Shinji Tanaka
  • Publication number: 20040219546
    Abstract: The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.
    Type: Application
    Filed: February 11, 2004
    Publication date: November 4, 2004
    Inventors: Shujiro Sakaki, Kenshiro Shuto, Mamoru Tsuchida, Yoshihiro Ashihara, Mitsuo Isomura
  • Publication number: 20030166302
    Abstract: The present invention provides an agglutination immunoassay, wherein the agglutination of insoluble carrier particles such as latex are stabilized and uniformized to give good reproducibility, and a reagent therefor. In the agglutination immunoassay which comprises allowing an antigenic substance in a sample to bind to insoluble carrier particles carrying substantially neither antigens nor antibodies thereon, and allowing an antibody or an antibody complex which reacts specifically to the antigenic substance to bind to the antigenic substance to give a selective agglutination of the insoluble carrier particles, a homopolymer prepared by polymerization of a monomer such as 2-methacryloyloxyethyl phosphorylcholine having a phosphorylcholine group and a vinyl group, or a copolymer prepared by polymerization of a monomer having a phosphorylcholine group and a vinyl group, with a monomer having a vinyl group such as n-butyl methacrylate is used.
    Type: Application
    Filed: February 28, 2003
    Publication date: September 4, 2003
    Inventors: Kayoko Shigenobu, Kenshiro Shuto, Shujiro Sakaki
  • Publication number: 20010026927
    Abstract: An object of the present invention is to provide a method and a reagent for measuring the subject substances containing high concentration of C-reactive protein without dilution while avoiding prozone phenomenon.
    Type: Application
    Filed: February 28, 2001
    Publication date: October 4, 2001
    Inventors: Hiroaki Yokohama, Harumi Umehara, Shigeru Matsumori, Satoshi Yamada, Kenshiro Shuto, Shujiro Sakaki, Ken Suzuki
  • Patent number: 5807942
    Abstract: A polymerized product of protein, comprising a polymer of one or more protein-containing monomers represented by the general formulaP.sub.r --?(Y).sub.k --(X).sub.m --R.sup.1 !.sub.n (1)wherein R.sup.1 represents a polymerizable group capable of causing a radical polymerization, P.sub.r denotes a protein residue, Y denotes a group formed from a functional group in said protein and the functional group R.sub.2 of the modifying agent given below, X denotes an organic divalent radical, k is zero or 1, m is zero or 1 and n is a numeral of 1 or higher,said protein-containing monomer being obtained by reacting a protein with a modifying agent represented by the general formula,R.sup.2 --(X).sub.m --R.sup.1 (2)wherein R.sub.2 represents a functional group capable of combining with a functional group of protein and R.sup.1, X and m are as given above.
    Type: Grant
    Filed: June 4, 1996
    Date of Patent: September 15, 1998
    Assignee: NOF Corporation
    Inventors: Shujiro Sakaki, Satoshi Yamada, Motohiro Mitani, Kenshiro Shuto, Yasumi Koinuma