Patents by Inventor Shuwei Yang
Shuwei Yang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240183147Abstract: A reconsolidated bamboo beam-column joint connected by a subassembly and a design method thereof are provided. Rectangular shear steel plates and vertical steel plates in embedded steel plates form an H-shaped section, and left and right ends of the vertical steel plates are embedded in mortises of a reconsolidated bamboo column and a reconsolidated bamboo beam respectively, thereby effectively improving bearing capacity of the joint. A half tenon is provided with an elliptical bolt hole in a penetrating manner and connected to the reconsolidated bamboo column by a bolt, and the half tenon is rotatable along a center of the elliptical bolt hole and movable along a long axis of the elliptical bolt hole, so that the energy is dissipated through friction between the reconsolidated bamboo half tenon and mortises, excellent energy consumption ability of the half tenon structure is exerted, and the anti-pulling resistance of the tenon is improved.Type: ApplicationFiled: August 16, 2023Publication date: June 6, 2024Applicant: Sichuan Provincial Architectural Design And Research Institute Co., Ltd.Inventors: Shixing ZHAO, Qiaoling ZHOU, Shuheng YANG, Yuanxu Tang, Ziqin ZHONG, Jing XIA, Fei HE, Qirui LUO, Dan LU, Feng XIONG, Ming ZHANG, Shuwei CHEN, Zhixiang YU, Jinqiu Qi, Yu YAO
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Publication number: 20240117451Abstract: Positive reference spiked in collected sample for use in qualitatively and quantitatively detecting viral RNA.Type: ApplicationFiled: March 10, 2021Publication date: April 11, 2024Inventors: Shuwei YANG, Liancheng HUANG, Feifei FENG, Longwen SU, Kun LIN, Can TANG, Chen LIANG, Yuanmei WANG, Yanqing CAI, Yilin PANG, Chuan SHEN, Zhixue YU
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Publication number: 20220090204Abstract: The present invention discloses a reference DNA and the use thereof, wherein the reference DNA is selected from the group consisting of: (i) DNA fragment 1: characterized in that it carries a defined gene mutation and at least one another artificially altered base X2, wherein, as compared to a wild type of the gene, at least one defined base X1 in the defined gene mutation undergoes a mutation associated with the occurrence, diagnosis, and/or treatment (such as a target targeted by a medicament) of a disease (such as a tumor), wherein the mutation is a substitution mutation, a deletion mutation, and/or an insertion mutation, and the artificially altered base X2 is different from the mutant base X1 which is contained in the DNA of a sample to be detected and defined to be associated with the occurrence, diagnosis and/or treatment of a disease, (ii) DNA fragment 2: characterized in that it differs from the DNA fragment 1 only in that it does not comprise the defined base X1 mutation, or (iii) a mixture of thType: ApplicationFiled: November 20, 2019Publication date: March 24, 2022Inventors: Shuwei Yang, Liancheng Huang, Chen Liang, Yunyi Chen, Haiying Chen
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Publication number: 20100167292Abstract: The present invention relates to a DNA and RNA polymerases which have increased fidelity (or reduced misincorporation rate). In particular, the invention relates to a method of making such polymerases by modifying or mutating the nucleotide binding domain of the polymerase (e.g., the O-helix). The invention also relates to DNA molecules containing the genes encoding the polymerases of the invention, to host cells containing such DNA molecules and to methods to make the polymerases using the host cells. The polymerases are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.Type: ApplicationFiled: September 14, 2009Publication date: July 1, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Shuwei Yang, Deb K. Chatterjee
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Patent number: 7501237Abstract: The present invention provides methods for use in identifying, analyzing and typing polymorphic DNA fragments, particularly minisatellite, microsatellite or STR DNA fragments. In particular, the invention provides methods using DNA polymerases, more particularly thermostable DNA polymerases, and most particularly Thermotoga polymerases or mutants or derivatives thereof, whereby minisatellite, microsatellite or STR DNA molecules may be amplified and analyzed for polymorphisms. The invention also relates to polymerases having reduced, substantially reduced or eliminated ability to add non-template 3? nucleotides to a synthesized nucleic acid molecule. In accordance with the invention, such reduction or elimination may be accomplished by modifying or mutating the desired polymerase.Type: GrantFiled: June 27, 2001Date of Patent: March 10, 2009Assignee: Life Technologies CorporationInventors: Joseph Solus, Shuwei Yang, Deb K Chatterjee
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Patent number: 7160702Abstract: Recombinant DNA vectors and methods for cloning and expressing nucleic acid molecules by using a combination of site-specific recombination and end-to-end joining or linking of nucleic acid molecules, such as endonuclease restriction digestion and ligation. The DNAs, vectors and methods can be used for inserting, exchanging, transferring a variety of DNA segment(s) both in vitro and in vivo. Also disclosed are linker molecules and methods using these linkers the can be used for cloning a gene of interest into an expression vector in one-step. The linker sequences comprise adapter sequences for cloning purposes, as well as eukaryotic and prokaryotic ribosome binding sites for increase translation efficiency.Type: GrantFiled: July 28, 2003Date of Patent: January 9, 2007Inventor: Shuwei Yang
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Publication number: 20060166334Abstract: A method for removing extraneous nucleotides in a cloned coding sequence using a type IIs endonuclease, the method comprising introducing a linker that comprises at least one recognition site for a Type IIs restriction endonuclease to the ORF, cloning the ORF into a suitable vector, and removing the extraneous nucleotides from the vector with a RE IIs digestion. Also provided are a vector, a kit and oligonucloetides suitable for the invention.Type: ApplicationFiled: December 21, 2005Publication date: July 27, 2006Applicant: GENECOPOEIA, INC.Inventor: Shuwei Yang
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Publication number: 20040115812Abstract: Recombinant DNA vectors and methods for cloning and expressing nucleic acid molecules by using a combination of site-specific recombination and end-to-end joining or linking of nucleic acid molecules, such as endonuclease restriction digestion and ligation. The DNAs, vectors and methods can be used for inserting, exchanging, transferring a variety of DNA segment(s) both in vitro and in vivo. Also disclosed are linker molecules and methods using these linkers the can be used for cloning a gene of interest into an expression vector in one-step. The linker sequences comprise adapter sequences for cloning purposes, as well as eukaryotic and prokaryotic ribosome binding sites for increase translation efficiency.Type: ApplicationFiled: July 28, 2003Publication date: June 17, 2004Applicant: GeneCopoeia, Inc.Inventor: Shuwei Yang
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Patent number: 6699981Abstract: The sensitivity and specificity of polynucleotide synthesis is increased by protecting the 3′-end of an oligonucleotide used as a primer in the synthesis of the polynucleotide. Protection of the 3′-end of an oligonucleotide prevents non-specific chain elongation. Removal of blocking group an elevated temperature, using a thermostable enzyme, permits template-specific polynucleotide synthesis. The present invention also provides oligonucleotides with a 3′ end protected by a blocking group and a thermostable enzyme capable of removing the blocking group at an elevated temperature. The compositions and methods of the invention are very useful in a variety of techniques for DNA/RNA amplification and analysis, including medical genetics research and diagnosis, pathogen detection, forensic, and animal and plant genetics applications, among others.Type: GrantFiled: May 14, 2001Date of Patent: March 2, 2004Assignee: GeneCopoeia, Inc.Inventor: Shuwei Yang
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Publication number: 20020182686Abstract: The sensitivity and specificity of polynucleotide synthesis is increased by protecting the 3′-end of an oligonucleotide used as a primer in the synthesis of the polynucleotide. Protection of the 3′-end of an oligonucleotide prevents non-specific chain elongation. Removal of blocking group an elevated temperature, using a thermostable enzyme, permits template-specific polynucleotide synthesis. The present invention also provides oligonucleotides with a 3′ end protected by a blocking group and a thermostable enzyme capable of removing the blocking group at an elevated temperature. The compositions and methods of the invention are very useful in a variety of techniques for DNA/RNA amplification and analysis, including medical genetics research and diagnosis, pathogen detection, forensic, and animal and plant genetics applications, among others.Type: ApplicationFiled: May 14, 2001Publication date: December 5, 2002Inventor: Shuwei Yang
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Publication number: 20020168646Abstract: The present invention provides methods for use in identifying, analyzing and typing polymorphic DNA fragments, particularly minisatellite, microsatellite or STR DNA fragments. In particular, the invention provides methods using DNA polymerases, more particularly thermostable DNA polymerases, and most particularly Thermotoga polymerases or mutants or derivatives thereof, whereby minisatellite, microsatellite or STRDNA molecules may be amplified and analyzed for polymorphisms. The invention also relates to polymerases having reduced, substantially reduced or eliminated ability to add non-template 3′ nucleotides to a synthesized nucleic acid molecule. In accordance with the invention, such reduction or elimination may be accomplished by modifying or mutating the desired polymerase.Type: ApplicationFiled: June 27, 2001Publication date: November 14, 2002Inventors: Joseph Solus, Shuwei Yang, Deb K. Chatterjee
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Patent number: 6306588Abstract: The present invention provides methods for use in identifying, analyzing and typing polymorphic DNA fragments, particularly minisatellite, microsatellite or STR DNA fragments. In particular, the invention provides methods using DNA polymerases, more particularly thermostable DNA polymerases, and most particularly Thermotoga polymerases or mutants or derivatives thereof, whereby minisatellite, microsatellite or STR DNA molecules maybe amplified and analyzed for polymorphisms. The invention also relates to polymerases having reduced, substantially reduced or eliminated ability to add non-template 3′ nucleotides to a synthesized nucleic acid molecule. In accordance with the invention, such reduction or elimination may be accomplished by modifying or mutating the desired polymerase.Type: GrantFiled: February 6, 1998Date of Patent: October 23, 2001Assignee: Invitrogen CorporationInventors: Joseph Solus, Shuwei Yang, Deb K. Chatterjee
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Patent number: 6274353Abstract: The sensitivity and specificity of polynucleotide synthesis is increased by protecting the 3′-end of an oligonucleotide used as a primer in the synthesis of the polynucleotide. Protection of the 3′-end of an oligonucleotide prevents non-specific chain elongation. Removal of blocking group an elevated temperature, using a thermostable enzyme, permits template-specific polynucleotide synthesis. The present invention also provides oligonucleotides with a 3′ end protected by a blocking group and a thermostable enzyme capable of removing the blocking group at an elevated temperature. The compositions and methods of the invention are very useful in a variety of techniques for DNA/RNA amplification and analysis, including medical genetics research and diagnosis, pathogen detection, forensic, and animal and plant genetics applications, among others.Type: GrantFiled: September 22, 1999Date of Patent: August 14, 2001Assignee: Genecopoeia, Inc.Inventor: Shuwei Yang