Abstract: Barbituric acid antigens are prepared by coupling 5- or 5,5-substituted barbituric acid to immunogenic carrier materials. In preferred embodiments, proteins are used as the carrier materials and the coupling is effected by an amide linkage between the 5-substituent of the barbituric acid and a carboxyl or amino group of the protein. The resulting antigens produce immunological effects when injected into host animals, including the formation of antibodies specific for 5- and 5,5-substituted barbituric acids. These specific antibodies are useful in bioanalytical techniques for the assay of barbituric acids in biological fluids.

Abstract: Barbituric acid antigens are prepared by coupling 5- or 5,5-substituted barbituric acid to immunogenic carrier materials. In preferred embodiments, proteins are used as the carrier materials and the coupling is effected by an amide linkage between the 5-substituent of the barbituric acid and a carboxyl or amino group of the protein. The resulting antigens produce immunological effects when injected into host animals, including the formation of antibodies specific for 5- and 5,5-substituted barbituric acids. These specific antibodies are useful in bioanalytical techniques for the assay of barbituric acids in biological fluids.

Abstract: A sensitive immunoassay for clonidine, 2-(2,6-dichloroanilino)-2-imidazoline hydrochloride is described. To prepare the clonidine selective antiserum, an antigen is made comprising 4-[[6-[2,4-dichloro-3(4,5-dihydro-1H-imidazol-2-yl)amino]hydroxyphenyl]azo ]benzoic acid covalently bonded to an immunogenic carrier material through a peptide bond formed from said carboxyl group and amino groups contained in said immunogenic carrier material and the antigen is injected into a suitable host animal to elicit the desired antiserum.

Abstract: A sensitive immunoassay for acetylcholine is described. To prepare the acetylcholine selective antiserum, an antigen is made comprising 6-aminocaproic acid (2-trimethylaminoethyl) ester covalently bonded to an immunogenic carrier material through a peptide bond formed from said 6-amino group and carboxyl groups contained in said immunogenic carrier material and the antigen is injected into a suitable host animal to elicit the desired antiserum.

Abstract: New hapten compositions useful in preparing antigens which may be employed in eliciting antibodies useful in immunoassay, particularly radioimmunoassay for propranolol. Utilizing an enantiomer in formation of the hapten allows preparation of an antibody which is able to discriminate between enantiomers selectively.

Abstract: A morphine-like compound (MLC) can be extracted from the brain of various species by utilizing morphine specific antibodies. MLC shows great similarities to morphine in immunological, chemical and chromatographic tests. The compound has biological activity as it inhibits the electrically induced contractions both of the guinea pig ileum and mouse vas deferens but the inhibition is not reversed by naloxone or naltrexone.

Abstract: New hapten compositions useful in preparing antigens which may be employed in eliciting antibodies useful in immunoassay, particularly radioimmunoassay for propranolol. Utilizing an enantiomer in formation of the hapten allows preparation of an antibody which is able to discriminate between enantiomers selectively.

Abstract: A sensitive radioimmunoassay for chlorpromazine is described. To prepare the chlorpromazine selective antiserum, an antigen is made comprising chlorpromazine covalently bonded to an immunogenic carrier material through a diazo containing linking group and the antigen is injected into a suitable host mammal to elicit the desired antiserum. A preferred linking group is diazophenylcarbonyl while bovine serum albumin is a preferred immunogenic carrier material.