Abstract: This disclosure provides a method of identifying or separating activated regulatory T cells that have a demethylated Foxp3 TSDR region from a mixture of activated T cells. The mixture is depleted of cells bearing the CD154 molecule (CD40 ligand). The desired cells can be recovered using a Treg marker such as CD25, GITR, CTLA4, CD137, latent TGF-beta (LAP), GARP (LRRC32) or CD121a/b. The Treg cells having a demethylated Foxp3 TSDR region can be used, for example for preparation of pharmaceutical compositions for use in treatment.

Abstract: The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, “latent TGF-beta (LAP)”, GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles.

Abstract: The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, “latent TGF-beta (LAP)”, GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles.

Abstract: A method for cleaning a vessel by dissolving iron oxide present in the vessel includes introducing a saline solution into the vessel, the saline solution being formed of an acid acting as a complexing agent, of an alkalizing agent and of a reducing agent, the saline solution acting as an alkaline reducer; retaining the saline solution in the vessel for a given period of time at a temperature above 140.degree. C. and below the decomposition temperature of the anionic part of the saline solution for forming an iron complex; and subsequently removing the dissolved iron complex by emptying the vessel.

Abstract: A process brings out a high contrast in natural grain of a naturally pale wood via infrared irradiation having a wavelength which is as long as possible. This infrared radiation can come from a conventional infrared emitter or, more preferably, from a carbon dioxide laser. Due to the contact-free heating near the surface which is effective in a stagnant ambient atmosphere, the new wood part of the grain is browned, while the harder old wood part remains essentially unbrowned. This causes the natural grain of the wood to stand out in contrast. Due to the contact-free and turbulence-free mode of operation of the heating, the grain pattern produced is uniform. Any scratches or chatter marks in the wood are simply covered over and remain invisible.

Abstract: A method for cleaning a vessel by dissolving iron oxide present in the vessel includes introducing a saline solution into the vessel, the saline solution being formed of an acid acting as a complexing agent, of an alkalizing agent and of a reducing agent, the saline solution acting as an alkaline reducer; retaining the saline solution in the vessel for a given period of time at a temperature above 140.degree. C. and below the decomposition temperature of the anionic part of the saline solution for forming an iron complex; and subsequently removing the dissolved iron complex by emptying the vessel.