Abstract: Disclosed is a well insert for cell culture and a method of manufacturing same, where the well insert has a membrane support made of a polyolefin material and having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein, and a permeable membrane sealed to the membrane for supporting a tissue culture, the permeable membrane being of brittle material and transparent in both air and water.

Abstract: Disclosed is a well insert for cell culture, including: a membrane support having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein; and a permeable membrane for supporting a tissue culture, the permeable membrane being attached at the lower end of the membrane support and sealed thereto, the permeable membrane being of brittle material. The membrane support is overmolded or fastened on to the permeable membrane so as be sealed thereto.

Abstract: Disclosed is a well insert for cell culture, including: a membrane support having an upper end and a lower end, the upper end being adapted to engage a well of a microplate so as to suspend the well insert therein; and a permeable membrane for supporting a tissue culture, the permeable membrane being attached at the lower end of the membrane support and sealed thereto, the permeable membrane being of brittle material. The membrane support is overmolded or fastened on to the permeable membrane so as be sealed thereto.

Abstract: A clamping insert for cell culture having a lower support with a hollow member engagable in a well of a microplate, the hollow member being open at both ends and including a support surface at one end, and first tightening elements. The clamping insert further having an inner holder sized to be fitted inside the hollow member, and second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate. The inner holder further including an open channel keeping free the porous substrate. Retaining elements intended to cooperate with the well of the microplate are provided to allow the clamping insert to be suspended in the well, and sealing elements are provided to hermetically seal the contact area between the clamping insert and the porous substrate.

Abstract: A clamping insert for cell culture having a lower support with a hollow member engagable in a well of a microplate, the hollow member being open at both ends and including a support surface at one end, and first tightening elements. The clamping insert further having an inner holder sized to be fitted inside the hollow member, and second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate. The inner holder further including an open channel keeping free the porous substrate. Retaining elements intended to cooperate with the well of the microplate are provided to allow the clamping insert to be suspended in the well, and sealing elements are provided to hermetically seal the contact area between the clamping insert and the porous substrate.

Abstract: A clamping insert for cell culture includes: a lower support having: i. a hollow member sized to be engaged in a well of a microplate, the hollow member being open at both its ends and including a support surface at one of its ends, ii. first tightening elements, an inner holder sized to be fitted inside the hollow member, and including second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate, the inner holder including an opened channel keeping free the porous substrate; retaining elements intended to cooperate with the well of the microplate to allow the clamping insert to be suspended in the well, sealing elements to hermetically seal the contact area between the clamping insert and the porous substrate.