Abstract: The present invention relates to a microarray device for detecting a target molecule such as miRNA in a sample. The device comprises a carrier substrate such as glass, an anti-fouling polymer layer which is functionalised with N-Hydroxysuccinimide (NHS) or carboxyl-groups and a capture probe. In an embodiment, the capture probe is an oligonucleotide with a stem-loop structure. The invention further defines a method for fabricating the device, a kit for detecting the target nucleic acid molecule comprising the device and a detection probe.

Abstract: The present invention relates to a microarray device for detecting a target molecule such as miRNA in a sample. The device comprises a carrier substrate such as glass, an anti-fouling polymer layer which is functionalised with N-Hydroxysuccinimide (NHS) or carboxyl-groups and a capture probe. In an embodiment, the capture probe is an oligonucleotide with a stem-loop structure. The invention further defines a method for fabricating the device, a kit for detecting the target nucleic acid molecule comprising the device and a detection probe.

Abstract: A method and system for detecting a DNA strand using carbon nanotubes or nanowires. A specific single strand of template DNA serves as a probe for its complementary strand in a solution containing DNA segments to be tested. The single-stranded sequence-specific DNA probe segment, whose ends are modified with amine, is attached between two carbon nanotubes/nanowires. When complementary strands representing DNA segments under test are brought near the probe strands, a dielectrophoresis (DEP) field may enhance the probability of selective hybridization between the complimentary target DNA and probe DNA. A change in electrical conductance in the probe strand occurs upon hybridization of the complementary target DNA with the single probe strand. This conductance change may be measured using the two carbon nanotubes or nano-dimensional electrodes. By exploiting nano-dimensional electrodes and single strand probe DNA, the proposed system is capable of accurately detecting a single molecule of DNA.

Abstract: The present invention is direct to a sensor for detecting a nucleic acid molecule comprising an electrode arrangement with two electrodes and nucleic acid probes immobilized at the surface of the electrodes. The present invention also refers to a kit and a method of using the sensor or a sensor array. The present invention is further directed to a process of manufacturing a sensor and sensor array.

Abstract: A method and system for detecting a DNA strand using carbon nanotubes or nanowires. A specific single strand of template DNA serves as a probe for its complementary strand in a solution containing DNA segments to be tested. The single-stranded sequence-specific DNA probe segment, whose ends are modified with amine, is attached between two carbon nanotubes/nanowires. When complementary strands representing DNA segments under test are brought near the probe strands, a dielectrophoresis (DEP) field may enhance the probability of selective hybridization between the complimentary target DNA and probe DNA. A change in electrical conductance in the probe strand occurs upon hybridization of the complementary target DNA with the single probe strand. This conductance change may be measured using the two carbon nanotubes or nano-dimensional electrodes. By exploiting nano-dimensional electrodes and single strand probe DNA, the proposed system is capable of accurately detecting a single molecule of DNA.