Abstract: A vehicle-to-everything (V2X) communication-based electronic toll collection system (ETCS) according to an embodiment of the present invention includes at least one roadside unit (RSU), a token issuer checking a de-identified token of a vehicle by communicating with an on-board unit (OBU) of the vehicle via the at least one roadside unit, and a clearing house communicating with the token issuer and charging a toll to a driver/owner of the vehicle. The token issuer generates toll information of the vehicle based on the de-identified token of the vehicle and location information of the at least one roadside unit involved in checking the de-identified token.

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

Abstract: A phosphoprotein extraction method and a mass spectrometric method using a microbore hollow fiber enzymatic reactor (mHFER) based antigen-antibody reaction and, specifically, to an extraction method and a mass spectrometric method, wherein phosphoproteins or phosphopeptides present in the body are extracted using phosphoserine-, phosphothreonine-, and phosphotyrosine-antibodies, and measured by a mass spectrometer, and thus biomarker phosphoproteins for diagnosis of diseases are found, contributing to early diagnosis of diseases.

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

Abstract: The present invention relates to a method for expressing, extracting and refining recombinant human growth hormone (hGH). More particularly, the present invention relates to a method for the intracellular expression of a target protein with minimizing the formation of insoluble inclusion bodies during the mass production of the protein in Escherichia coli system. The present invention also relates to a method for extracting and refining the target protein, particularly human growth hormone, with maximizing its solubility during the extraction and with providing a high yield but without losing the biological activity of the target protein.

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

Abstract: A method of erasing and programming a flash memory device including multi-level cells (MLCs). MLCs of a word line are selected and some of the MLCs are pre-programmed based on whether their individual threshold voltages are included in a first voltage range. The selected MLCs are pre-programmed with a pre-program (first) voltage; and the remaining MLCs are prohibited from pre-programming; then the remaining MLCs connected to the selected word line are programmed by applying a program (second) voltage that gradually rises from the pre-program voltage at a ratio of a step voltage n for the selected line.

Abstract: Erase and program methods of a flash memory device including MLCs for increasing the program speed. In the erase method, MLCs are pre-programmed so that a voltage range in which threshold voltages of MLCs are distributed can be reduced. Therefore, a fail occurrence ratio can be reduced when erasing MLCs, the threshold voltage distribution of MLCs can be improved and an overall program time can be shortened in a subsequent program operation.

Abstract: Erase and program methods of a flash memory device including MLCs for increasing the program speed are described. In the erase method, MLCs are pre-programmed so that a voltage range in which threshold voltages of MLCs are distributed can be reduced. Therefore, a fail occurrence ratio can be reduced when erasing MLCs, the threshold voltage distribution of MLCs can be improved and an overall program time can be shortened in a subsequent program operation.

Abstract: Erase and program methods of a flash memory device including MLCs for increasing the program speed are described. In the erase method, MLCs are pre-programmed so that a voltage range in which threshold voltages of MLCs are distributed can be reduced. Therefore, a fail occurrence ratio can be reduced when erasing MLCs, the threshold voltage distribution of MLCs can be improved and an overall program time can be shortened in a subsequent program operation.

Abstract: The present invention provides a novel fluorescent chemosensor including a naphthalene diimide-Zn(II) complex, which can efficiently recognize pyrophosphate (PPi) at pH 7.4. The fluorescent chemosensor exhibits a new kind of excimer fluorescence, which is selective for PPi in 100% aqueous solution. Further, since the naphthalene diimide-Zn(II) complex of the present invention does not couple with inorganic phosphate (Pi) or adenosine triphosphate (ATP) and has high selectivity for PPi, it can be usefully used to detect pyrophosphate (PPi), which serves to transfer signals and store energy in living organisms.

Abstract: Erase and program methods of a flash memory device including MLCs for increasing the program speed are described. In the erase method, MLCs are pre-programmed so that a voltage range in which threshold voltages of MLCs are distributed can be reduced. Therefore, a fail occurrence ratio can be reduced when erasing MLCs, the threshold voltage distribution of MLCs can be improved and an overall program time can be shortened in a subsequent program operation.

Abstract: The present invention provides a novel fluorescent chemosensor including a naphthalene diimide-Zn(II) complex, which can efficiently recognize pyrophosphate (PPi) at pH 7.4. The fluorescent chemosensor exhibits a new kind of excimer fluorescence, which is selective for PPi in 100% aqueous solution. Further, since the naphthalene diimide-Zn(II) complex of the present invention does not couple with inorganic phosphate (Pi) or adenosine triphosphate (ATP) and has high selectivity for PPi, it can be usefully used to detect pyrophosphate (PPi), which serves to transfer signals and store energy in living organisms.