Abstract: A disposable urine collection device for use by pet owners and veterinarians on small animals such as dogs is disclosed. The urine collection device of the present invention is formed from opposed sheets of a flexible plastic material such as polyethylene being hermetically sealed about the peripheral edges of thereof to form a collection bag. The collection bag is provided with a continuous pressure actuated closure at the top of the bag so as to retain a urine specimen in a sanitary manner. A supporter including a plurality of upwardly extending leg bands is integrally formed with the collection bag to secure the device about the hind quarters of the dog or other animal. In one embodiment the elongated leg bands are adapted to be hand tied about the girth and hind quarters of the animal. In an alternative embodiment the leg bands are provided with detachable fasteners which are utilized to attach the urine collection device of the present invention in its functional position.