Patents by Inventor Steen Troels J.o slashed.rgensen

Steen Troels J.o slashed.rgensen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Procaryotic cell comprising at least two copies of a gene
    Patent number: 6100063
    Abstract: A prokaryotic cell expressing a gene of interest and comprising at least two copies of said gene on the chromosome.
    Type: Grant
    Filed: February 11, 1999
    Date of Patent: August 8, 2000
    Assignee: Novo Nordisk A/S
    Inventor: Steen Troels J.o slashed.rgensen
  • Production of bacillus thuringiensis integrants
    Patent number: 5955367
    Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.
    Type: Grant
    Filed: April 14, 1998
    Date of Patent: September 21, 1999
    Assignee: Abbott Laboratories
    Inventors: Lee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels J.o slashed.rgensen, Per Lin.ang. J.o slashed.rgensen, B.o slashed.rge Krag Diderichsen
  • Method of homologous recombination followed by in vivo selection of DNA amplification
    Patent number: 5925544
    Abstract: A method of amplifying in vivo a DNA sequence B present in a genome of a parent cell, comprising (a) integrating in a genome of said cell a DNA construct comprising the structure C-M-A-D, in which both A and C denote a DNA sequence which is homologous with a genomic DNA fragment either flanking or overlapping the DNA sequence B, the sequence C being located in the opposite end the sequence B as compared to A, D denotes a DNA sequence which is homologous with a genomic DNA fragment located distal for C as compared to B, and M denotes a DNA sequence encoding a selection marker, (b) selecting for cells in which the DNA sequence M has been integrated in the genome, which cells comprise, in any orientation, the structure A-B-C-M-A-D and (c) propagating the cells selected in step (b) under increasing selection pressure to obtain a cell which has obtained an increased number of genomically integrated copies of the DNA sequences B and M.
    Type: Grant
    Filed: October 20, 1997
    Date of Patent: July 20, 1999
    Assignee: Novo Nordisk A/S
    Inventor: Steen Troels J.o slashed.rgensen
  • DNA integration by transposition
    Patent number: 5882888
    Abstract: Multicopy strains of gram-positive bacteria carrying multiple copies of a DNA sequence of interest may be constructed by use of a method involving introduction of a DNA construct comprising the DNA sequence of interest into the genome of the recipient cell by transposition and subsequent deletion of a marker gene used for selection of the cells having received the DNA construct by a resolution system. The multicopy strains are preferably free from a gene encoding an undesirable marker such as an antibiotic resistance marker.
    Type: Grant
    Filed: July 17, 1997
    Date of Patent: March 16, 1999
    Assignee: Novo Nordisk A/S
    Inventor: Steen Troels J.o slashed.rgensen
  • Amplification of genomic DNA by site specific integration of a selectable marker construct
    Patent number: 5733753
    Abstract: A method of amplifying in vivo a DNA sequence B present in a genome of a parent cell, comprising (a) integrating in a genome of said cell a DNA construct comprising the structure C-M-A-D, in which both A and C denote a DNA sequence which is homologous with a genomic DNA fragment either flanking or overlapping the DNA sequence B, the sequence C being located in the opposite end the sequence B as compared to A, D denotes a DNA sequence which is homologous with a genomic DNA fragment located distal for C as compared to B, and M denotes a DNA sequence encoding a selection marker, (b) selecting for cells in which the DNA sequence M has been integrated in the genome, which cells comprise, in any orientation, the structure A-B-C-M-A-D and (c) propagating the cells selected in step (b) under increasing selection pressure to obtain a cell which has obtained an increased number of genomically integrated copies of the DNA sequences B and M.
    Type: Grant
    Filed: November 18, 1996
    Date of Patent: March 31, 1998
    Assignee: Novo Nordisk A/S
    Inventor: Steen Troels J.o slashed.rgensen
  • Bacillus promoter derived from a variant of a bacillus licheniformis X-amylase promoter
    Patent number: 5698415
    Abstract: A Bacillus promoter included in DNA sequence (SEQ ID#1), wherein each of N.sup.1 -N.sup.9 is A, T, C or G with the exception that N.sup.2 -N.sup.9 do not together form the sequence ATGTTTCA or GTGTTTCA, or a functional homologue of said sequence.
    Type: Grant
    Filed: May 10, 1994
    Date of Patent: December 16, 1997
    Assignee: Novo Nordisk Als
    Inventors: Steen Troels J.o slashed.rgensen, B.o slashed.rge Krag Diderichsen
  • Stable integration of DNA in bacterial genomes
    Patent number: 5695976
    Abstract: A bacterial cell which in its genome carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, the DNA construct lacking a functional gene coding for a factor required to initiate replication from the origin of replication.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: December 9, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Steen Troels J.o slashed.rgensen, Per Lin.ang. J.o slashed.rgensen, B.o slashed.rge Krag Diderichsen
  • Process for preparing lipases
    Patent number: 5681715
    Abstract: A process for producing an active lipase enzyme in vitro, comprising mixing an inactive or partly active lipase enzyme with a chaperone molecule and subjecting the mixture to denaturation followed by renaturation to produce the active lipase enzyme.
    Type: Grant
    Filed: March 3, 1995
    Date of Patent: October 28, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Steen Troels J.o slashed.rgensen, Boerge Krag Diderichsen, Catherine M. Buckley, Audrey Hobson, David J. McConnell