Patents by Inventor Stefan Hell
Stefan Hell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220026434Abstract: The present invention relates to improved (simplified/easier, more robust and more reproducible) methods for identification of carbohydrates compositions, e.g. out of complex carbohydrate mixtures, as well as the determination of carbohydrate mixture composition patterns (e.g.: of glycosylation patterns) based on advanced internal standards to determine precise and highly reproducible migration and retention time indices using novel fluorescent dyes in combination with high performance separation technologies, like capillary (gel) electrophoresis (C(G)E) or (ultra)high performance liquid chromatography (U)HPLC with a highly sensitive detection like (laser induced) fluorescence detection.Type: ApplicationFiled: January 21, 2019Publication date: January 27, 2022Inventors: Erdmann RAPP, René HENNIG, Udo REICHL, Stefan HELL, Vladimir BELOV, Matthias BISCHOFF, Dirk MEINEKE, Laura THOMAS, Kirill KOLMAKOV, Gyuzel MITRONOVA, Elizaveta SAVICHEVA
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Patent number: 10174202Abstract: Compounds of formula I are disclosed: wherein X1, X2, X3, X4 are independently H, F, Cl, Br, I, CN, NO2, OR1, SR1, NR1R2, COR1, COOR1, CONR1R2, PO3R1R2, SO2R1, SO3R1 or R3; R1 and R2 are, e.g., H, alkyl or aryl or optionally a ring; R3 is, e.g., alkyl, alkenyl, alkynyl, aryl or cycloalkyl; Y is OR1, NR1R2, or NR1R3; Q is O, S, SO2, NR, C(R3)2, Si(R3)2, Ge(R3)2, P(?O)R3 or P(?O)OR3; Q and X1 can optionally form part of a ring; L and M are independently OR1, SR1, NR1R2 and R3; L and M can optionally form part of a ring; Z is O, S, NR1, CR1R3 or aryl; and Z and X4 can optionally form part of a ring.Type: GrantFiled: February 1, 2018Date of Patent: January 8, 2019Assignee: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E. V.Inventors: Maksim Sednev, Alexey Butkevich, Heydar Shojaei, Vladimir Belov, Stefan Hell, Christian Wurm, Dirk Kamin
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Publication number: 20180223102Abstract: Compounds of formula I are disclosed: wherein X1, X2, X3, X4 are independently H, F, Cl, Br, I, CN, NO2, OR1, SR1, NR1R2, COR1, COOR1, CONR1R2, PO3R1R2, SO2R1, SO3R1 or R3; R1 and R2 are, e.g., H, alkyl or aryl or optionally a ring; R3 is, e.g., alkyl, alkenyl, alkynyl, aryl or cycloalkyl; Y is OR1, NR1R2, or NR1R3; Q is O, S, SO2, NR, C(R3)2, Si(R3)2, Ge(R3)2, P(?O)R3 or P(?O)OR3; Q and X1 can optionally form part of a ring; L and M are independently OR1, SR1, NR1R2 and R3; L and M can optionally form part of a ring; Z is O, S, NR1, CR1R3 or aryl; and Z and X4 can optionally form part of a ring.Type: ApplicationFiled: February 1, 2018Publication date: August 9, 2018Inventors: Maksim SEDNEV, Alexey BUTKEVICH, Heydar SHOJAEI, Vladimir BELOV, Stefan HELL, Christian WURM, Dirk KAMIN
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Patent number: 8580579Abstract: The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy.Type: GrantFiled: April 26, 2010Date of Patent: November 12, 2013Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan Hell, Vladimir N. Belov, Kirill Kolmakov, Volker Westphal, Marcel Lauterbach, Stefan Jakobs, Christian Wurm, Christian Eggeling, Christian Ringemann
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Publication number: 20120100559Abstract: The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications.Type: ApplicationFiled: April 26, 2010Publication date: April 26, 2012Applicant: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan Hell, Vladimir N. Belov, Kirill Kolmakov, Volker Westphal, Marcel Lauterbach, Stefan Jakobs, Christian Wurm, Christian Eggeling, Christian Ringemann
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Patent number: 8084754Abstract: For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.Type: GrantFiled: January 25, 2011Date of Patent: December 27, 2011Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Publication number: 20110160083Abstract: For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.Type: ApplicationFiled: January 25, 2011Publication date: June 30, 2011Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Patent number: 7897937Abstract: For imaging a structure in a sample with spatial resolution, the structure is labeled with a fluorophore which is transferable by an optical transfer signal out of a first into a second photochromic state. Via a common objective, the sample is subjected to both the focussed optical transfer signal and a focussed optical excitation signal only exciting a portion of the fluorophore being in its second photochromic state for fluorescence. The transfer and the excitation signal have a common centre of maximum intensity; and a decrease of intensity of the transfer signal with the distance to this common centre is substantially stronger than any decrease of the effective return rate of the fluorophore back into the first photochromic state. Fluorescence light emitted by the excited fluorophore is detected. Then, the common centre is shifted with regard to the sample; and the steps of subjecting and detecting are repeated.Type: GrantFiled: March 30, 2009Date of Patent: March 1, 2011Assignees: Deutsches Krebsforschungszentrum, Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Johann Engelhardt, Marion Lang, Stefan Hell
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Patent number: 7894067Abstract: For the high spatial resolution imaging of a structure in a sample (2) the structure is marked with a substance which can be changed over by means of a first electromagnetic signal (5) from a first state having a larger absorption cross section for a second electromagnetic signal (3) into a second state having a smaller absorption cross section for the second signal (3) or which can be changed over by means of a first electromagnetic signal (5) into a first state having a larger absorption cross section for a second electromagnetic signal (3) from a second state having a smaller absorption cross section for the second signal (3). A spatially delimited distribution of a portion of the substance in the first state is then set by means of the first signal (5). Afterward, the second electromagnetic signal (3) is applied to the sample (2), and a local temperature increase in the sample (2) which results from the larger absorption cross section of the substance in the first state is detected.Type: GrantFiled: June 17, 2009Date of Patent: February 22, 2011Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventor: Stefan Hell
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Patent number: 7880150Abstract: For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e.Type: GrantFiled: May 29, 2008Date of Patent: February 1, 2011Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Patent number: 7764369Abstract: A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present.Type: GrantFiled: September 18, 2007Date of Patent: July 27, 2010Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Volker Westphal, Norbert Quaas
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Patent number: 7719679Abstract: The inventive method for optically measuring a sample consists in temporarily repeatedly transmitting an electromagnetic signal (2) to the sample in such a way that a substance contained in the sample is transferred from a first electronic state (1) into a second electronic state (3), wherein at least one part of said substance in the second state (3) emits photons which are used for carrying out the optical measurement of the sample, the signal (2) is transmitted to the same sample area at a certain repetition interval and said repetition interval of the signal (2) is adjusted with a lifetime of the second state (3) of the substance having an order of magnitude of 1 ns on a value of at least 0.1 ?s which is optimized with respect to photon yield from the substance.Type: GrantFiled: December 13, 2007Date of Patent: May 18, 2010Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Gerald Donnert
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Publication number: 20090279086Abstract: For the high spatial resolution imaging of a structure in a sample (2) the structure is marked with a substance which can be changed over by means of a first electromagnetic signal (5) from a first state having a larger absorption cross section for a second electromagnetic signal (3) into a second state having a smaller absorption cross section for the second signal (3) or which can be changed over by means of a first electromagnetic signal (5) into a first state having a larger absorption cross section for a second electromagnetic signal (3) from a second state having a smaller absorption cross section for the second signal (3). A spatially delimited distribution of a portion of the substance in the first state is then set by means of the first signal (5). Afterward, the second electromagnetic signal (3) is applied to the sample (2), and a local temperature increase in the sample (2) which results from the larger absorption cross section of the substance in the first state is detected.Type: ApplicationFiled: June 17, 2009Publication date: November 12, 2009Applicant: Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventor: Stefan HELL
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Publication number: 20090242801Abstract: For imaging a structure in a sample with three-dimensional spatial resolution, a fluorophore is selected which is transferable by means of an optical transfer signal out of a first into a second photochromic state having specific fluorescence properties, and which displays a return rate back into the first photochromic state. The structure is labeled with the fluorophore.Type: ApplicationFiled: March 30, 2009Publication date: October 1, 2009Applicants: DEUTSCHES KREBSFORSCHUNGSZENTRUM, Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventors: Johann ENGELHARDT, Marion LANG, Stefan HELL
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Publication number: 20090134342Abstract: For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e.Type: ApplicationFiled: May 29, 2008Publication date: May 28, 2009Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Folling, Andreas Schonle, Mariano Bossi
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Patent number: 7539115Abstract: In a method of creating a permanent structure with high spatial resolution, a substance which may be modified by an optical signal is provided in a writing area. The optical signal is applied to the writing area in such a way that a spatially limited partial area of the writing area is purposefully omitted, the spatially limited partial area being a local intensity minimum of the optical signal, and the optical signal, outside of the spatially limited partial area, being applied to the writing area in such a way that saturation is achieved in modifying the substance with the optical signal. Then, different states of the substance in the spatially limited partial area and of the substance in the partial areas of the writing area covered by the optical signal are permanently adjusted.Type: GrantFiled: October 12, 2005Date of Patent: May 26, 2009Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventor: Stefan Hell
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Patent number: 7538893Abstract: A method of microscopically examining a spatial fine structure comprises the steps of selecting a luminophore from the group of luminophores which have two physical states, the two states differing from each other with regard to the luminescence properties displayed by the luminophore, and which are reversibly, but essentially completely transferable out of one into the other state of their two states by means of an optical signal; overlaying a surface of the spatial fine structure with the luminophore; and determining the profile of the surface overlaid with the luminophore. The step of determining the profile of the surface comprises the sub-steps of transferring the luminophore by means of the optical signal out of the one into the other of its two states outside a presently observed measurement point, measuring luminescence light emitted by the luminophore, and repeating the sub-steps of transferring and measuring for further measurement points distributed over the surface.Type: GrantFiled: September 26, 2007Date of Patent: May 26, 2009Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Volker Westphal, Norbert Quaas
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Patent number: 7430045Abstract: In high spatial resolution imaging, a structure of a sample is marked with a substance selected from a group of substances capable of being repeatedly transferred out of a first state having first optical properties into a second state having second optical properties by means of an optical switch over signal, and capable of returning out of the second state into the first state, the two states differing in at least one criteria. Within areas the sample is transferred into the second state by means of the optical switch over signal with which at least one spatially limited area is purposefully omitted. An optical measurement signal is detected, which is associated with the substance in the first state and which comes out of a detection area including both the area omitted with the switch over signal and areas in which the substance has been transferred into to second state.Type: GrantFiled: October 12, 2005Date of Patent: September 30, 2008Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.Inventor: Stefan Hell
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Publication number: 20080088839Abstract: The inventive method for optically measuring a sample consists in temporarily repeatedly transmitting an electromagnetic signal (2) to the sample in such a way that a substance contained in the sample is transferred from a first electronic state (1) into a second electronic state (3), wherein at least one part of said substance in the second state (3) emits photons which are used for carrying out the optical measurement of the sample, the signal (2) is transmitted to the same sample area at a certain repetition interval and said repetition interval of the signal (2) is adjusted with a lifetime of the second state (3) of the substance having an order of magnitude of 1 ns on a value of at least 0.1 ?s which is optimized with respect to photon yield from the substance.Type: ApplicationFiled: December 13, 2007Publication date: April 17, 2008Applicant: Max Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Gerald Donnert
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Publication number: 20080018891Abstract: A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present.Type: ApplicationFiled: September 18, 2007Publication date: January 24, 2008Applicant: Max-Planck-Gesellschaft zur forderung der Wissenschaften e.V.Inventors: Stefan Hell, Volker Westphal, Norbert Quaas