Abstract: Disclosed herein include systems, methods, compositions, and kits for the combined analysis of circulating cell-free nucleic acids and single cells in peripheral blood. The method can comprise isolating cell-free nucleic acids (cfNA), immune cells, leukocytes, and/or circulating tumor cells (CTCs) from a biological sample derived from a subject (e.g., a blood sample). The method can comprise performing high-throughput single cell sequencing assays. The method can comprise generating values for one or more genomic properties, one or more expression properties, and/or one or more variant properties based on sequence reads generated from said sequencing assays. Cancer prediction scores, MRD scores, and/or therapeutic efficacy scores can be generated based on the values of said properties. The methods provided herein can yield improved sensitivity and specificity in non-invasive blood-based oncology diagnostics.

Abstract: The presently disclosed subject matter relates to technology and methods for analyzing the structure of RNA molecules. More particularly, the presently disclosed subject matter is directed to methods of, compositions for, and computer program products for RNA structure analysis through alkoxide-selective 2?-hydroxyl acylation analyzed by primer extension.

Abstract: The presently disclosed subject matter relates to technology and methods for analyzing the structure of RNA molecules. More particularly, the presently disclosed subject matter is directed to methods of, compositions for, and computer program products for RNA structure analysis through alkoxide-selective 2?-hydroxyl acylation analyzed by primer extension.