Patents by Inventor Steffen Schaffer
Steffen Schaffer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180273990Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: ApplicationFiled: March 12, 2018Publication date: September 27, 2018Inventors: Katrin GRAMMANN, Jan WOLTER, Liv REINECKE, Steffen SCHAFFER, Eileen E. SPINDLER, Wendy K. RIBBLE, Brittany L. PRATHER, Catherine B. POOR, Tanya Warnecke LIPSCOMB, Hans H. LIAO, Dave A. HOGSETT, Ronald J. EVANS
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Publication number: 20180245107Abstract: There is provided a method of oxidising at least one organic substance in aerobic conditions to produce at least one alcohol, amine, acid, aldehyde, and/or ketone, the method comprising: (a) producing ethanol and/or acetate from a carbon source in aerobic conditions, comprising (i) contacting the carbon source with a reaction mixture comprising —a first acetogenic microorganism in an exponential growth phase; —free oxygen; and —a second acetogenic microorganism in a stationary phase, wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol; and (b) contacting the acetate and/or ethanol from step (a) with the organic substance and with a third microorganism capable of oxidising the organic substance to produce the alcohol, amine, acid, aldehyde, and/or ketone and wherein the acetate is a co-substrate.Type: ApplicationFiled: November 16, 2016Publication date: August 30, 2018Applicant: EVONIK DEGUSSA GMBHInventors: Thomas HAAS, Simon BECK, Steffen SCHAFFER
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Publication number: 20180127791Abstract: The present invention relates to a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise; a first genetic mutation that enables the cell to produce at least one acyl amino acid and; a second genetic mutation that enables the cell to decrease glutamate breakdown relative to the wild type cell.Type: ApplicationFiled: April 4, 2016Publication date: May 10, 2018Applicant: Evonik Degussa GmbHInventors: Steffen SCHAFFER, Katrin GRAMMANN, Liv REINECKE
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Patent number: 9944959Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: GrantFiled: September 2, 2015Date of Patent: April 17, 2018Assignee: CARGILL, INCORPORATEDInventors: Katrin Grammann, Jan Wolter, Liv Reinecke, Steffen Schaffer, Eileen C. Spindler, Wendy K. Ribble, Brittany L. Prather, Catherine B. Poor, Tanya Warnecke Lipscomb, Hans H. Liao, Dave A. Hogsett, Ronald J. Evans
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Patent number: 9765370Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.Type: GrantFiled: March 25, 2013Date of Patent: September 19, 2017Assignee: Evonik Degussa GmbHInventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
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Patent number: 9725746Abstract: The invention relates to a whole-cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an ?-dioxygenase and contacting the product with a transaminase.Type: GrantFiled: December 18, 2013Date of Patent: August 8, 2017Assignee: Evonik Degussa GmbHInventors: Steffen Schaffer, Jasmin Corthals, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Michael Volland, Martin Roos
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Patent number: 9719117Abstract: The invention provides a whole cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a ?-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.Type: GrantFiled: December 18, 2013Date of Patent: August 1, 2017Assignee: Evonik DegussaInventors: Steffen Schaffer, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
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Publication number: 20170145448Abstract: There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of (i) Enzyme E1 capable of converting the alkane to the corresponding 1-alkanol; (ii) Enzyme E2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) Enzyme E3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) Enzyme E4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) Enzyme E5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester, and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E20-E24 selected from the group consisting of: E20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.Type: ApplicationFiled: November 23, 2016Publication date: May 25, 2017Applicant: Evonik Degussa GmbHInventors: Steffen SCHAFFER, Thomas Haas, Wilhelm Bruegging, Ralf Meier
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Publication number: 20170130248Abstract: The present invention relates to a cell for producing acyl glycinates wherein the cell is genetically modified to comprise at least a first genetic mutation that increases the expression relative to the wild type cell of an amino acid-N-acyl-transferase, at least a second genetic mutation that increases the expression relative to the wild type cell of an acyl-CoA synthetase, and at least a third genetic mutation that decreases the expression relative to the wild type cell of at least one enzyme selected from the group consisting of an enzyme of the glycine cleavage system, glycine hydroxymethyltransferase (GlyA) and threonine aldolase (LtaE).Type: ApplicationFiled: April 28, 2015Publication date: May 11, 2017Applicant: Evonik Degussa GmbHInventors: LIV REINECKE, STEFFEN SCHAFFER, KATRIN GRAMMANN, MAIK OLFERT, NICOLE DECKER, NILS ARTO, HANS-GEORG HENNEMANN
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Publication number: 20170096695Abstract: The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an ?/? hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, and wherein the carbon source is a C4 molecule.Type: ApplicationFiled: April 27, 2015Publication date: April 6, 2017Inventors: OLIVER THUM, PHILIP ENGEL, CHRISTIAN GEHRING, STEFFEN SCHAFFER, MIRJA WESSEL
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Patent number: 9611489Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C —(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.Type: GrantFiled: March 12, 2013Date of Patent: April 4, 2017Assignee: EVONIK DEGUSSA GmbHInventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
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Patent number: 9598711Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.Type: GrantFiled: June 27, 2016Date of Patent: March 21, 2017Assignee: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
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Patent number: 9580720Abstract: This invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids.Type: GrantFiled: March 10, 2015Date of Patent: February 28, 2017Assignee: EVONIK DEGUSSA GMBHInventors: Steffen Schaffer, Mirja Wessel, Anja Thiessenhusen, Nadine Stein
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Patent number: 9580732Abstract: A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.Type: GrantFiled: July 17, 2012Date of Patent: February 28, 2017Assignee: Evonik Degussa GmbHInventors: Markus Pötter, Thomas Haas, Jan Christoph Pfeffer, Arne Skerra, Wolfgang Kroutil, Alexandra Lerchner, Johann H. Sattler, Steffen Schaffer, Katharina Christin Tauber
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Publication number: 20160304916Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.Type: ApplicationFiled: June 27, 2016Publication date: October 20, 2016Applicant: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
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Publication number: 20160272950Abstract: The present invention relates to a cell expressing an amino acid-N-acyl-transferase, which is preferably recombinant, and an acyl-CoA synthetase, which is preferably recombinant, wherein the cell has a reduced fatty acid degradation capacity, a method for producing acyl amino acids, comprising the step contacting an amino acid and an acyl CoA in the presence of an amino acid-N-acyl-transferase, which is preferably isolated and/or recombinant, wherein the amino acid-N-acyl-transferase is preferably a human amino acid-N-acyl-transferase, or culturing the cell and a reaction mixture comprising an amino acid-N-acyl-transferase, which is preferably isolated and/or recombinant, an acyl-CoA synthetase, which is preferably isolated and/or recombinant, an amino acid and either a fatty acid-CoA or a fatty acid and an acyl-CoA-synthase.Type: ApplicationFiled: August 25, 2014Publication date: September 22, 2016Applicant: EVONIK DEGUSSA GMBHInventors: Jasmin CORTHALS, Katrin GRAMMANN, Thomas HAAS, Maik OLFERT, Nicole POTGRAVE, Liv REINECKE, Steffen SCHAFFER, Jan WOLTER
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Patent number: 9404118Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.Type: GrantFiled: July 23, 2012Date of Patent: August 2, 2016Assignee: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
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Patent number: 9388439Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.Type: GrantFiled: March 13, 2013Date of Patent: July 12, 2016Assignee: EVONIK DEGUSSA GMBHInventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
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Publication number: 20160138058Abstract: The invention relates to a method for producing organic compositions comprising the method steps: A) providing an oxyhydrogen bacterium having an activity of an enzyme E1, which is increased by comparison with the wild type thereof and which can catalyse the conversion of 2 acetyl-CoA to acetoacetyl-CoA and CoA, in an aqueous medium; B) bringing the aqueous medium into contact with a gas containing H2, CO2 and O2 in a weight ratio from 20-70 to 10-45 to 5-35 and optionally C) purifying the organic composition.Type: ApplicationFiled: May 30, 2014Publication date: May 19, 2016Applicant: Evonik Degussa GmbHInventors: Eva Maria WITTMANN, Thomas HAAS, Steffen SCHAFFER, Markus POETTER, Yvonne SCHIEMANN, Nigole KIRCHNER
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Publication number: 20160138061Abstract: At least one fatty acid and/or derivative thereof is produced from a gas containing H2, CO2, and O2 by providing a genetically modified hydrogen oxidizing bacterium in an aqueous medium; and contacting the aqueous medium with the gas containing H2, CO2 and O2 in a weight ratio of 20 to 70 (H2): 10 to 45 (CO2): 5 to 35 (O2); wherein the fatty acid contains at least 5 carbon atoms and wherein the hydrogen oxidizing bacterium is genetically modified relative to the wild type bacterium to increase the expression of enzyme E1 that is capable of catalyzing the conversion of acetyl CoA to acyl ACP via malonyl coA and to increase the expression of enzyme E2 that is capable of catalyzing the conversion of Acyl ACP to the fatty acid.Type: ApplicationFiled: November 17, 2015Publication date: May 19, 2016Applicant: Evonik Degussa GmbHInventors: Thomas HAAS, Markus Poetter, Steffen Schaffer