Abstract: A method of synthesizing saccharide compositions is described. In this method, an acceptor moiety is contacted with at least one donor saccharide in the presence of at least one cell surface-bound glycosyltransferase specific for catalyzing the coupling of the acceptor moiety with the donor saccharide. The acceptor moiety used is a carbohydrate, a protein, a glycolipid, a lipid, or a glycolipid.

Abstract: A reiterative method for obtaining the glycosyltransferases useful for glycosyltransferase-catalyzed synthesis of a saccharide composition by serially bonding preselected saccharide units onto an immobilized acceptor moiety which is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids and carbohydrates.

Abstract: A method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula I(NeuAc-.alpha.(2-3)-pGal-.beta.(1)--(--X--).sub.m --(--Y--).sub.n --).sub.p --ZwhereinX=a chemical bond or a group capable of linking the p galactose to either the linking group Y or the multivalent support Z;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C;Y=a linking group;Z=a multivalent support;m=0 or 1;n=0 or 1; andp=an integer of 2-1,000 is described. Also described is a method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula IINeuAc-.alpha.(214 3)-pGal-.beta.(1)-AwhereinA=a group capable of bonding to the p galactose;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C.

Abstract: A composition for treating and/or inhibiting gastric and duodenal ulcers, comprising an oligosaccharide selected from the group consisting of Formula I ##STR1## or a mixture thereof; wherein:X is independently OH or NHAc;Y is independently H, or an amino acid or a peptide of 2-100, preferably 2-20, amino acids; andW, W', and W" are each independently H or ##STR2## where Z is independently H or a pharmaceutically acceptable cation; and P is independently H or ##STR3## where Z is defined as above; wherein at least one of W, W' or W" is an .alpha.-N-acetylneuraminic acid moiety, andwherein W' and W" are not simultaneously .alpha.-N-acetylneuraminic acid moietywith the proviso that the compound of Formula II is not NAN .alpha.(2.fwdarw.3)Gal .beta.1-4 Glu or NAN .alpha.(2.fwdarw.6)Gal .beta.1-4 Glu; and a kit for detecting the presence of Helicobacter pylori comprising at least one compound of the Formula I or II, are described.

Abstract: This invention relates to an apparatus containing specific binary combinations of glycosyltransferases, for the synthesis of specific saccharide compositions such as, for example, oligosaccharides, polysaccharides, glycolipids, and glycopeptides.

Abstract: A method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula I(NeuAc-.alpha.(2-3)-Gal-.beta.(1)-(--X--).sub.m -(--Y--).sub.n -).sub.p -ZwhereinX=a chemical bond or a group capable of linking the galactose to either the linking group Y or the multivalent support Z;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C;Y=a linking group;Z=a multivalent support;m=0 or 1;n=0 or 1; andp=an integer of 2-1,000 is described. Also described is a method for treating and/or inhibiting gastric and duodenal ulcers, comprising administering a pharmaceutical composition comprising an oligosaccharide of Formula IINeuAc-.alpha.(2-3)-Gal-.beta.(1)-AwhereinA=a group capable of bonding to the galactose;wherein the C.sub.1 glycosidic oxygen of galactose may be replaced by N, S or C.

Abstract: An apparatus for a glycosyltransferase-catalyzed synthesis of a tetrasaccharide composition of the formula gal-glcNAc-gal-B-1,4-glc from saccharide units and an acceptor moiety. The apparatus has a reactor, an inlet, an outlet, and contains two glycosyltransferases; an N-acetyl glucosaminyltransferase and a galactosyltransferase.

Abstract: An apparatus for focusing an image onto an image receptor is disclosed. The apparatus includes a stationary lens, a rotatable frame that is rigidly connected to the image receptor, and a base. The base is pivotally connected to the rotatable frame about a rotational axis. The image is focused by the stationary lens onto the image receptor by pivoting the rotatable frame about the rotational axis. In an alternate embodiment, an apparatus for focusing an image onto an image receptor includes a stationary lens, a voice coil actuator, a servo loop for driving the voice coil actuator, and an electromechanical transducer for measuring the position of the image receptor. The electromechanical transducer is electrically connected to the servo loop. Connecting means for rigidly connecting the image receptor to the voice coil actuator are also provided. The image is focused by the stationary lens onto the image receptor by driving the voice coil actuator.

Abstract: A method and an apparatus for preparing saccharide compositions is disclosed. The mGOVERNMENT SUPPORTPortions of this invention were supported by National Science Foundation Grant DCB8817883.

Abstract: A dual function antenna system and method. A system is provided for using a single antenna for both the reception of low frequency navigational signals, such as LORAN-C, and high frequency communications signals in the VHF region. The antenna is at a remote location from a low frequency receiver and a high frequency transceiver. Both the low frequency and the high frequency signals are coupled to the receiver and transceiver, respectively, over the same transmission line at one end thereof. A preamplifier is provided at the location of the antenna to increase the strength of the low frequency signal applied to the transmission line. A high pass filter is provided to couple the antenna and the transmission line and prevent feedback from the output of the preamplifier from reaching its input. The other end of the transmission line is coupled to the high frequency transceiver by a high pass filter, and to the low frequency receiver by a low pass filter.

Abstract: A magnetometer and method for measuring magnetic field strength particularly adapted for use in an electronic flux gate compass. A core of magnetic material in the shape of a toroid is coupled to a drive coil and to two sense coils oriented along orthogonal axes. A current limited, current source coupled to the drive coil periodically saturates the core, drives the core out of saturation at an intermediate current level, and saturates the core again. Each sense coil is coupled to a sense circuit which measures the current in the sense coil induced by the periodically changing flux density in the coil resulting from an external magnetic field coupled to the sense coil, produces a dc feedback signal to cancel out the external magnetic field, and provides the dc signal as an output representative of the external magnetic field strength along the axis of the sense coil. The dc signal is produced by synchronously detecting the amplified ac current in the sense coil.

Abstract: A variable attenuator comprises a plurality of attenuator stages connected in series along a transmission line signal path. Each attenuator stage comprises a resstive pi-section attenuating pad which may be selectively inserted into the signal path to increase attenuation along the path. Each series element of the pad is selectively inserted or removed from the signal path by opening or closing a parallel connected switching device, while each shunt element of the pad is selectively connected to or disconnected from ground by closing or opening a corresponding series connected switching device. The switching devices each comprise an NPN bipolar transistor and a PNP bipolar transistor, the emitter of each transistor being coupled to the emitter of the other and the collector or each transistor being connected to the collector of the other, to provide a low impedance AC signal path from collector to emitter when the transistors are active.