Abstract: Provided herein are methods for target gene sequencing and single cell barcoding in conjunction with analysis of gene expression in single cells. In some embodiments, the target gene is an immune molecule, such as an antibody or TCR. In some embodiments, the methods can be used to carry out transcriptome sequencing, e.g., RNA sequencing, to capture transcriptome of single cells paired with full receptor immune receptor sequences such that information about the immune repertoire and transcriptome of a cell can be determined. Also provided are polynucleotide libraries for use in carrying out transcriptome analysis and immune molecule, e.g., antibody or TCR, sequencing.

Abstract: Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences, for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.

Abstract: Apparatus (10, 200) for the provision of TNP therapy to a wound is described, the apparatus (10, 200) including: a waste canister (22, 204) into which fluid is aspirated from a wound by aspiration pump means (44, 248) and a fluid flow path (420, 422) for at least a part of said aspirated fluid on an outlet side of said aspiration pump means (44) wherein said fluid flow path (420, 422) on the outlet side comprises a silencing system (400) having a plurality of silencing elements (402, 404, 406) therein.

Abstract: Receiving and processing user orders may include receiving an order for an order quantity of a product from a user computing device, retrieving a threshold inventory quantity of the product, retrieving a last known inventory quantity of the product, comparing the threshold inventory quantity with the difference between the last known inventory quantity and the order quantity, performing an asynchronous reservation of the quantity of the product when the threshold inventory quantity is less than the difference between the last known inventory quantity and the order quantity, and performing a synchronous reservation of the quantity of the product when the threshold inventory quantity is greater than the difference between the last known inventory quantity and the order quantity.

Abstract: Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences, for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.

Abstract: Methods and apparatuses for detecting full waste canister and/or fluid flow path blockage conditions are disclosed. Also disclosed are methods and apparatuses for controlling a pump. In some embodiments, flow of fluid can be restricted in a portion of the fluid flow path. A controller can be configured to compare a difference in pressure values upstream and downstream of a fluid flow restrictor to a pressure difference threshold, and determine based on the comparison whether to activate an alarm indicating the full waste canister condition or the fluid flow path blockage condition. The controller can be additionally or alternatively configured to determine a fluid flow using a flow meter, open a selectable valve in response to a comparison of the fluid flow with a fluid flow threshold, determine fluid flow after opening the valve, and determine based on the fluid flow after opening the valve whether to activate the alarm.

Abstract: Receiving and processing user orders may include receiving an order for an order quantity of a product from a user computing device, retrieving a threshold inventory quantity of the product, retrieving a last known inventory quantity of the product, comparing the threshold inventory quantity with the difference between the last known inventory quantity and the order quantity, performing an asynchronous reservation of the quantity of the product when the threshold inventory quantity is less than the difference between the last known inventory quantity and the order quantity, and performing a synchronous reservation of the quantity of the product when the threshold inventory quantity is greater than the difference between the last known inventory quantity and the order quantity.

Abstract: Provided herein are binding molecules, such as those that recognize or bind a peptide epitope of a cancer antigen, such as expressed on a cancer cell, including cells infected with human papilloma virus (HPV) or that contain HPV DNA sequences and/or those that recognize or bind a peptide epitope of HPV 16 E6 or E7, in the context of a major histocompatibility complex (MHC) molecule. Among the provided binding molecules are T cell receptors (TCRs) or antibodies, including antigen-binding fragments thereof, that bind or recognize such peptide epitopes. The present disclosure further relates to engineered cells comprising such binding molecules, e.g., TCRs or antibodies (and chimeric antigen receptors containing the antibodies), and uses thereof in adoptive cell therapy.

Abstract: Methods and apparatuses for detecting full waste canister and/or fluid flow path blockage conditions are disclosed. Also disclosed are methods and apparatuses for controlling a pump. In some embodiments, flow of fluid can be restricted in a portion of the fluid flow path. A controller can be configured to compare a difference in pressure values upstream and downstream of a fluid flow restrictor to a pressure difference threshold, and determine based on the comparison whether to activate an alarm indicating the full waste canister condition or the fluid flow path blockage condition. The controller can be additionally or alternatively configured to determine a fluid flow using a flow meter, open a selectable valve in response to a comparison of the fluid flow with a fluid flow threshold, determine fluid flow after opening the valve, and determine based on the fluid flow after opening the valve whether to activate the alarm.

Abstract: In an example embodiment, there is disclosed herein a sleeve that has a peel-off appliqué or label that includes desired graphics that can be combined with a drink cup or container. The label or appliqué or “collectable” acts as a “mini-poster” that is removable and repositionable. The label may have graphics of sports starts or other things such as musical performers, super heroes, etc. Particular embodiments include interior die cut images within the main graphic as “collectibles within a collectible.

Abstract: Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, for antibody discovery, disease and immune diagnostics, and low error sequencing.

Abstract: In an example embodiment, there is disclosed herein a sleeve that has a peel-off appliqué or label that includes desired graphics that can be combined with a drink cup or container. The label or appliqué or “collectable” acts as a “mini-poster” that is removable and repositionable. The label may have graphics of sports starts or other things such as musical performers, super heroes, etc. Particular embodiments include interior die cut images within the main graphic as “collectibles within a collectible.” In another example embodiment, a container appliqué is applied to the container.

Abstract: A method and system for monitoring a signal from a function to record a set of values for each signal monitored; determining for each signal monitored the relative value of the signal being monitored over time; identifying each signal exhibiting a relative value representing a change in a pre-identified direction and each signal exhibiting a relative value not representing a change in the pre-identified direction, wherein each signal is aligned in the same direction relative to the pre-identified direction; dynamically weighting at least one signal monitored; displaying the monitored signals as an aggregate including enhancement of each signal exhibiting a change in the pre-identified direction; and causing a desired change in at least one monitored function based upon feedback of the displayed aggregate signals.

Abstract: The present invention includes a method for optical character recognition of external segmented displays using a camera connected to a reading device and a data reading application on the reading device.

Abstract: A micro-fluidic device includes a plurality of heating elements on a substrate for heating the substrate. They define a plurality of temperature regions having distinct temperatures on the substrate. A flow feature layer is formed above the substrate to define a channel extending across the substrate through each temperature region. As fluid is repeatedly pumped within the channel, it flows from one temperature region to a next temperature region to undergo thermal cycling. Fluid wells reside in the flow feature layer under a cover and connect to the channel whereby fluid can dwell for diagnostic applications.

Abstract: Apparatus (10, 200) for the provision of TNP therapy to a wound is described, the apparatus (10, 200) including: a waste canister (22, 204) into which fluid is aspirated from a wound by aspiration pump means (44, 248) and a fluid flow path (420, 422) for at least a part of said aspirated fluid on an outlet side of said aspiration pump means (44) wherein said fluid flow path (420, 422) on the outlet side comprises a silencing system (400) having a plurality of silencing elements (402, 404, 406) therein.

Abstract: A system and method for distributing reminder information in an electronic network includes an information service that is configured to provide information distribution services through said information distribution network. User devices are provided for device users to communicate with the information service for receiving the information distribution services. A transport structure communicates with network entities in the information distribution network for collecting appropriate types of metadata. A transport server then performs a metadata analysis procedure upon the various types of metadata to responsively generate the reminder information.

Abstract: A method for processing user orders for products may include receiving an order for an order quantity of the product from a user computing device, determining that the order management system cannot communicate with the inventory management system to reserve the order quantity of the product, retrieving a last known inventory quantity of the product, retrieving an offline demand quantity for the product, transmitting a confirmation of the order to the user computing device when the sum of the order quantity and the offline demand quantity of the product is less than or equal to the last known inventory quantity of the product, and transmitting a denial of the order to the user computing device when the sum of the order quantity and the offline demand quantity of the product is greater than the last known inventory quantity of the product.

Abstract: Receiving and processing user orders may include receiving an order for an order quantity of a product from a user computing device, retrieving a threshold inventory quantity of the product, retrieving a last known inventory quantity of the product, comparing the threshold inventory quantity with the difference between the last known inventory quantity and the order quantity, performing an asynchronous reservation of the quantity of the product when the threshold inventory quantity is less than the difference between the last known inventory quantity and the order quantity, and performing a synchronous reservation of the quantity of the product when the threshold inventory quantity is greater than the difference between the last known inventory quantity and the order quantity.