Patents by Inventor Stephen Mayfield
Stephen Mayfield has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230167225Abstract: Disclosed herein are bio-based polymers, methods to prepare the same, bio-based polymer products, methods to degrade the same, and methods to recycle the same.Type: ApplicationFiled: April 26, 2021Publication date: June 1, 2023Inventors: Stephen Mayfield, Robert Pomeroy, Michael Burkart, Natasha Gunawan, Ariel Schreiman, Marissa Tessman, Nitin Neelakantan, Ryan Simkovsky
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Patent number: 11549095Abstract: Provided herein are methods for the production of high density cultures of algae as well as nutritional supplements from such cultures and the use of such cultures to produce therapeutic proteins.Type: GrantFiled: August 14, 2017Date of Patent: January 10, 2023Assignee: Triton Algae Innovations, Inc.Inventors: Robert McBride, Oscar Gonzalez, Stephen Mayfield, Miller Tran, Xun Wang, Jon Hansen
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Publication number: 20200370004Abstract: The present disclosure provides for growing algae with an exogenous organic carbon source as the primary carbon source, in light, dark or limited light conditions. Also provided are expression cassettes for expression of a recombinant protein in an algae species grown in dark or limited light conditions.Type: ApplicationFiled: November 13, 2018Publication date: November 26, 2020Inventors: Miller Tran, Jon Hansen, John Deaton, Xun Wang, Oscar Gonzalez, Michael Mayfield, Stephen Mayfield
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Publication number: 20190183161Abstract: Provided herein are methods for the production of high density cultures of algae as well as nutritional supplements from such cultures and the use of such cultures to produce therapeutic proteins.Type: ApplicationFiled: August 14, 2017Publication date: June 20, 2019Inventors: Robert McBride, Oscar Gonzalez, Stephen Mayfield, Miller Tran, Xun Wang, Jon Hansen
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Patent number: 8669059Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: GrantFiled: August 14, 2012Date of Patent: March 11, 2014Assignees: The Scripps Research Institute, Sapphire Energy, Inc.Inventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Kari Mikkelson, Yan Poon
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Publication number: 20120309014Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: ApplicationFiled: August 14, 2012Publication date: December 6, 2012Applicants: The Scripps Research Institute, Sapphire Energy, Inc.Inventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Kari Mikkelson, Yan Poon
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Patent number: 8318436Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: GrantFiled: October 7, 2011Date of Patent: November 27, 2012Assignees: Sapphire Energy, Inc., The Scripps Research InstituteInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
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Patent number: 8268553Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: GrantFiled: May 30, 2008Date of Patent: September 18, 2012Assignees: Sapphire Energy, Inc., The Scripps Research InstituteInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Kari Mikkelson, Yan Poon
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Publication number: 20120107853Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: ApplicationFiled: October 7, 2011Publication date: May 3, 2012Applicants: THE SCRIPPS RESEARCH INSTITUTE, SAPPHIRE ENERGY, INC.Inventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
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Patent number: 8143039Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: GrantFiled: May 30, 2008Date of Patent: March 27, 2012Assignees: Sapphire Energy, Inc., The Scripps Research InstituteInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
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Publication number: 20090280545Abstract: The present invention provides compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.Type: ApplicationFiled: September 10, 2008Publication date: November 12, 2009Applicants: Sapphire Energy, The Scripps Research InstituteInventors: Michael Mendez, Stephen Mayfield, Bryan O'Neill, Yan Poon, Phillip Lee, Craig Aaron Behnke, Su-Chiung Fang
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Publication number: 20090253169Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: ApplicationFiled: May 30, 2008Publication date: October 8, 2009Applicants: SAPPHIRE ENERGY, THE SCRIPPS RESEARCH INSTITUTEInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
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Publication number: 20090246766Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.Type: ApplicationFiled: May 30, 2008Publication date: October 1, 2009Applicants: Sapphire Energy, The Scripps Research InstituteInventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Kari Mikkelson, Yan Poon
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Publication number: 20070298050Abstract: Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of combinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional acitivity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.Type: ApplicationFiled: May 9, 2007Publication date: December 27, 2007Applicant: The Scripps Research InstituteInventor: Stephen Mayfield
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Patent number: 6294653Abstract: The present invention relates to recombinant protein disulfide isomerase, RB60, that functions as a translational regulator of the binding of a translational activator protein, RB47, to its binding site for the activation of translation. The recombinant RB60 protein is useful in a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants.Type: GrantFiled: July 10, 2000Date of Patent: September 25, 2001Assignee: The Scripps Research InstituteInventor: Stephen Mayfield
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Patent number: 6156517Abstract: The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.Type: GrantFiled: July 13, 1999Date of Patent: December 5, 2000Assignee: The Scripps Research InstituteInventor: Stephen Mayfield