Abstract: There is provided an animal training system to train an animal to detect a training odor. The animal training system comprises a target (310) comprising the training odor (320) and a hidden marker (330); and a detector (350) for identifying the hidden marker. There is further provided an animal training method using the animal training system, the animal training method for practice by a supervisor, an animal handler, and an animal. The animal training method comprises placing the target (310) in a location of a search area (300), the target comprising the training odor (320) and the hidden marker (330), the placing being carried out by the supervisor; introducing the animal handler and the animal (305) to the search area; scanning with the detector (350) a candidate location for the hidden marker, the candidate location being a location that is indicated by the animal; and rewarding the animal if the detector indicates the presence of the hidden marker at the candidate location.

Abstract: There is provided an animal training system to train an animal to detect a training odour. The animal training system comprises a target (310) comprising the training odour (320) and a hidden marker (330); and a detector (350) for identifying the hidden marker. There is further provided an animal training method using the animal training system, the animal training method for practice by a supervisor, an animal handler, and an animal. The animal training method comprises placing the target (310) in a location of a search area (300), the target comprising the training odour (320) and the hidden marker (330), the placing being carried out by the supervisor; introducing the animal handler and the animal (305) to the search area; scanning with the detector (350) a candid ate location for the hidden marker, the candidate location being a location that is indicated by the animal; and rewarding the animal if the detector indicates the presence of the hidden marker at the candidate location.

Abstract: A process for detecting an analyte which process comprises (a) contacting a sample suspected of containing said analyte with a containment means comprising a barrier which separates signal generating reagents from said sample, in the presence of an element which interacts specifically with said analyte, under conditions whereby interaction between the analyte and the said element results in activation of the signal generating reagents within the containment means on the side of the barrier opposite to the sample, and (b) detecting any signal generated and retained within the containment means from the sample side of the barrier. The process of the invention provides for sensitive detection of very small numbers of analyte materials using measurement techniques which include counting methods such as flow cytometry.

Abstract: An enzymically active cell free extract or membrane fraction capable of catalysing the removal of nitrite from hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is provided. The cell free extract is produced by culturing a strain of Rhodococcus rhodochrous bacterium from nature. The strain designated 11Y has been deposited as NCIMB 40820. Methods of bioremediation using the enzymically active cell free extract so produced are also provided. Additionally there is provided a method for detecting the presence of RDX in a sample together with a biosensor for use in such a method. The nitrite produced in the enzyme catalysed reaction with RDX is detected, for example, colorimetrically, or alternatively a product of RDX degradation, such as formal behyde, is detected.

Abstract: An enzyme capable of catalyzing the removal of nitrite from pentaerythritol tetranitrate (PETN) is provided. The enzyme (known as PETN reductase enzyme) is produced by culturing a novel strain of the Enterobacter cloacae bacterium isolated from nature. The strain designated PB2 has been deposited as NCIMB 40718. The amino acid sequence of the enzyme and the genetic sequence which encodes for this enzyme have also been determined. A PETN reductase enzyme encoded by the on gene is provided. A method for producing PETN reductase enzyme in large quantities and methods of bioremediation using the enzyme so produced are also provided. Additionally there is provided a method of detecting the presence of PETN in a sample together with a biosensor for use in such a method.