Patents by Inventor Stephen Van Dien
Stephen Van Dien has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230158088Abstract: Provided are compositions, including products of manufacture and kits, and methods, comprising combinations of microbes, such as non-pathogenic, live bacteria and/or bacterial spores, for the control, amelioration, prevention, and treatment of a disease or condition, for example, a viral infection such as a COVID19 infection, and these non-pathogenic, live bacteria and/or bacterial spores can be administered to an individual, thereby resulting in a modification or modulation of the individual's gut microfloral population(s), and by modulating or modifying the individual's gut microbial population(s) using compositions, products of manufacture and methods as provided herein, the pharmacodynamics or effectiveness of a drug or a vaccine administered to the individual is altered, for example, the pharmacodynamics of the drug or vaccine is enhanced, the individual's ability to absorb a drug is modified or the dose efficacy of a drug or vaccine is increased.Type: ApplicationFiled: March 26, 2021Publication date: May 25, 2023Inventors: Stephanie J. CULLER, Robert J. HASELBECK, Stephen VAN DIEN
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Publication number: 20220378855Abstract: Provided are compositions, including products of manufacture and kits, and methods, comprising combinations of microbes, such as non-pathogenic, live bacteria and/or bacterial spores, for the control, amelioration, prevention, and treatment of a disease or condition, for example, a cancer. In alternative embodiment, these non-pathogenic, live bacteria and/or bacterial spores are administered to an individual in need thereof, thereby resulting in a modification or modulation of the individual's gut microfloral population(s). In alternative embodiments, by modulating or modifying the individual's gut microbial population(s) using compositions, products of manufacture and methods as provided herein, the pharmacodynamics of a drug administered to the individual is altered, thereby controlling, ameliorating, preventing and/or treating of that cancer.Type: ApplicationFiled: December 17, 2020Publication date: December 1, 2022Inventors: Stephanie J. CULLER, Robert J. HASELBECK, Stephen VAN DIEN, Anandh SWAMINATHAN, Hirokazu SATO
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Patent number: 10844404Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.Type: GrantFiled: April 7, 2016Date of Patent: November 24, 2020Assignee: Genomatica Inc.Inventors: Stephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen Van Dien
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Publication number: 20180135082Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.Type: ApplicationFiled: April 7, 2016Publication date: May 17, 2018Applicant: GENOMATICA, iNC.Inventors: Stephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen Van Dien
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Patent number: 8030036Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.Type: GrantFiled: October 24, 2007Date of Patent: October 4, 2011Assignee: Ajinomoto Co., Inc.Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Yuta Nakai, Tomoko Suzuki, Mika Moriya, Yuichiro Tsuji, Takuji Ueda
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Patent number: 7809511Abstract: A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.Type: GrantFiled: January 24, 2006Date of Patent: October 5, 2010Assignee: Ajinomoto Co., Inc.Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Takuji Ueda, Yuichiro Tsuji
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Patent number: 7555392Abstract: A method for determining metabolic flux distribution of a cell, which comprises the steps of: 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate to a product, 2) computing a set of vectors of solutions existing in a solution space which the stoichiometric matrix may have, 3) selecting, from the computed set of vectors of solutions, maximum vectors whose vector elements corresponding to respective substances for which input values can be obtained are maximum, and 4) performing linear combination for the selected vectors in accordance with the following equations to obtain a vector representing metabolic flux distribution: S flux = ? i = 1 n ? a i · p i · S ? ( i ) + ? i = 1 n ? b i · q i · A ? ( i ) ( I ) ? i = 1 n ? a i + ? i = 1 n ? b i = 1 ( II ) Sflux: Vector representing metabolic flux distribution to be determined, S(i): Vector selected for substance for which inputted value cType: GrantFiled: April 1, 2005Date of Patent: June 30, 2009Assignee: Ajinomoto Co., Inc.Inventors: Shintaro Iwatani, Stephen Van Dien, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20090148915Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.Type: ApplicationFiled: October 24, 2007Publication date: June 11, 2009Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Yuta Nakai, Tomoko Suzuki, Mika Moriya, Yuichiro Tsuji, Takuji Ueda
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Patent number: 7306933Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.Type: GrantFiled: January 3, 2006Date of Patent: December 11, 2007Assignee: Ajinomoto Co., Inc.Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Yuta Nakai, Tomoko Suzuki, Mika Moriya, Yuichiro Tsuji, Takuji Ueda
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Publication number: 20070111294Abstract: The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions encoding an enzyme associated with growth-coupled production of succinate when an activity of the enzyme is reduced, whereby the one or more gene disruptions confers stable growth-coupled production of succinate onto the non- naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling succinate production to growth of the microorganism, the set of metabolic modifications comprising disruption of one or more genes selected from the set of genes comprising: (a) adhE, ldhA; (b) adhE, ldhA, acka-pta; (c) pfl, ldhA; (d) pfl, ldhA, adhE; (e) acka-pta, pykF, atpF, sdhA; (f) acka-pta, pykF, ptsG, or (g) acka-pta, pykF, ptsG, adhE, ldhA, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of succinate.Type: ApplicationFiled: September 8, 2006Publication date: May 17, 2007Inventors: Anthony Burgard, Stephen Van Dien
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Publication number: 20060154344Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.Type: ApplicationFiled: January 3, 2006Publication date: July 13, 2006Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Yuta Nakai, Tomoko Suzuki, Mika Moriya, Yuichiro Tsuji, Takuji Ueda
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Publication number: 20060105322Abstract: A method for analyzing an intracellular metabolic flux comprising determining the intracellular metabolic flux from analytical values of cells cultured in a medium containing an isotope-labeled substrate as a carbon source on the basis of an intracellular metabolic flux model constructed for the intracellular metabolic flux to be analyzed, wherein (a) influence of an exchange reaction between an intracellular metabolite and a cell component produced by integration of the intracellular metabolite is considered, (b) uptake of a compound in a medium into cells, which compound is identical to an intracellular metabolite and unlabeled with an isotope, is considered, or (c) carbon dioxide used in a fixation reaction is assumed as carbon dioxide produced in a production reaction.Type: ApplicationFiled: December 28, 2005Publication date: May 18, 2006Inventors: Shintaro Iwatani, Stephen Van Dien, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20050221278Abstract: A method for determining metabolic flux distribution of a cell, which comprises the steps of: 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate to a product, 2) computing a set of vectors of solutions existing in a solution space which the stoichiometric matrix may have, 3) selecting, from the computed set of vectors of solutions, maximum vectors whose vector elements corresponding to respective substances for which input values can be obtained are maximum, and 4) performing linear combination for the selected vectors in accordance with the following equations to obtain a vector representing metabolic flux distribution: S flux = ? i = 1 n ? a i · p i · S ? ( i ) + ? i = 1 n ? b i · q i · A ? ( i ) ( I ) ? i = 1 n ? a i + ? i = 1 n ? b i = 1 ( II ) Sflux: Vector representing metabolic flux distribution to be determined, S(i): Vector selected for substance for which inputted value canType: ApplicationFiled: April 1, 2005Publication date: October 6, 2005Inventors: Shintaro Iwatani, Stephen Van Dien, Yoshihiro Usuda, Kazuhiko Matsui