Patents by Inventor Stevan Bogdan Jovanovich
Stevan Bogdan Jovanovich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11926815Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.Type: GrantFiled: March 9, 2023Date of Patent: March 12, 2024Assignee: S2 GENOMICS, INC.Inventors: Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
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Publication number: 20240027311Abstract: This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.Type: ApplicationFiled: August 1, 2023Publication date: January 25, 2024Inventors: Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Publication number: 20240026338Abstract: A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.Type: ApplicationFiled: May 22, 2023Publication date: January 25, 2024Inventors: Stevan Bogdan Jovanovich, Peter Wagner
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Publication number: 20230407232Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.Type: ApplicationFiled: March 9, 2023Publication date: December 21, 2023Inventors: Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
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Patent number: 11719608Abstract: This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.Type: GrantFiled: January 23, 2022Date of Patent: August 8, 2023Assignee: S2 GENOMICS, INC.Inventors: Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Publication number: 20230184641Abstract: A system, methods, and apparatus are described to collect and prepare cells, nuclei, subcellular components, and biomolecules from specimens including FFPE and OCT preserved tissues. The system can perform deparaffinization, rehydration, enzymatic and/or chemical and physical disruption of the FFPE tissue, or residue removal of the OCT tissue, to dissociate it into a single cell or nuclei suspension.Type: ApplicationFiled: May 18, 2021Publication date: June 15, 2023Inventors: Stevan Bogdan Jovanovich, John Bashkin, Nathan Pereira
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Patent number: 11667907Abstract: A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.Type: GrantFiled: July 21, 2021Date of Patent: June 6, 2023Assignee: SILICON VALLEY SCIENTIFIC, INC.Inventors: Stevan Bogdan Jovanovich, Peter Wagner
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Patent number: 11618876Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.Type: GrantFiled: June 1, 2019Date of Patent: April 4, 2023Assignee: S2 GENOMICS, INC.Inventors: Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
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Publication number: 20220290092Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.Type: ApplicationFiled: June 1, 2019Publication date: September 15, 2022Inventors: Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
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Patent number: 11441976Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced.Type: GrantFiled: October 28, 2021Date of Patent: September 13, 2022Assignee: S2 GENOMICS, INC.Inventors: Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Publication number: 20220146382Abstract: This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.Type: ApplicationFiled: January 23, 2022Publication date: May 12, 2022Inventors: Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Publication number: 20220082479Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced.Type: ApplicationFiled: October 28, 2021Publication date: March 17, 2022Inventors: Stevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Publication number: 20220033802Abstract: A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.Type: ApplicationFiled: July 21, 2021Publication date: February 3, 2022Inventors: Stevan Bogdan Jovanovich, Peter Wagner
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Publication number: 20210214673Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.Type: ApplicationFiled: June 1, 2019Publication date: July 15, 2021Inventors: Stevan Bogdan Jovanovich, Kaiwan Chear, Bruce Leisz, David Eberhart, John Bashkin
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Publication number: 20210046482Abstract: Provided herein is a system comprising: a) spatial sampler system configured to collect and transmit one or a plurality of cells or nuclei from a tissue specimen, the system comprising: i) a lower carrier having an array of conduits passing therethrough, each conduit comprising an opening on a first side and communicating with an opening on a second side, wherein the opening on the second side either (1) terminates in a capillary or (2) opens onto a well of a multiwell plate; ii) positioned, above the lower carrier, a perforated specimen holder configured to support a frozen tissue specimen, wherein the specimen holder comprises a plurality of perforations having a size sufficient to permit the passage of single cells or nuclei; and iii) positioned, above the specimen holder, a multifunctional head comprising an upper array of upper conduits, optionally, each aligned with a conduit opening of the lower carrier.Type: ApplicationFiled: March 12, 2019Publication date: February 18, 2021Inventor: Stevan Bogdan JOVANOVICH
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Patent number: 9752185Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. Also disclosed in the present disclosure is a flow through, traveling-wave, bead-beating device which comprises a rotating pole piece, a flow through tube, and a magnetic piece. Rotation of the rotating pole piece may create a magnetic wave down the flow through tube, thereby producing sufficient acceleration of beads through the tube to disrupt or lyse target analytes flowing through the tube.Type: GrantFiled: August 12, 2015Date of Patent: September 5, 2017Assignee: IntegenX Inc.Inventors: Allen Boronkay, Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20160053314Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: ApplicationFiled: August 12, 2015Publication date: February 25, 2016Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga, Allen Boronkay
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Patent number: 9121058Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.Type: GrantFiled: August 20, 2011Date of Patent: September 1, 2015Assignee: IntegenX Inc.Inventors: Seth Stern, Stevan Bogdan Jovanovich
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Publication number: 20140045704Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.Type: ApplicationFiled: May 2, 2013Publication date: February 13, 2014Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, luliu I. Balga, Allen R. Boronkay, Joanne Horn, Michael Van Nguyen, William D. Nielsen, Mattias Vangbo
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Publication number: 20130344475Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: ApplicationFiled: May 17, 2013Publication date: December 26, 2013Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga, Allen Boronkay