Abstract: Method and apparatus for measuring fluid motion with micron scale spatial resolution, in which fluorescent particles are injected into a fluid body in a test device, the test device is broadly illuminated with pulses of light at the excitation frequency of the fluorescent particles, the fluorescent light is collected by a microscope objective lens, and the light thus collected is relayed through a fluorescent filter to an image recording device, the depth of field of the objective lens defining the thickness of a two-dimensional measurement plane.

Abstract: A method and apparatus for measuring fluid motion with micron scale spatial resolution has been developed. Here, micron or submicron solid fluorescent particles are injected into the fluid body. The particles are chosen to closely follow the motion of the fluid. Pulses of light, at the excitation wavelength, broadly illuminate the test device and the fluorescent particles, The flourescent particles absorb the excitation light and emit pulses of fluorescent light, at the emission wavelength. The fluorescent light is collected by a microscope objective lens, and relayed through a fluorescent filter to an image recording device, such as a CCD camera. Subsequently, discrete images of discrete particles at two or more instances in time are recorded. These images can then be analyzed using correlation analysis to obtain velocity measurements.