Patents by Inventor Steven A. Ripp
Steven A. Ripp has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240093206Abstract: A system for stable expression of gene pathways in cell lines, methods of making cell lines with stable expression of gene pathways, and methods of using the same are disclosed herein. The system comprises a nucleic acid construct configured to encode at least two genes of a multigene pathway in a cell. The nucleic acid construct comprises a plurality of nucleic acid sequences, wherein the plurality of nucleic acid sequences comprises: a first nucleic acid sequence encoding at least one gene of the multigene pathway; a first protease recognition nucleic acid sequence encoding a protease recognition site; a first linker nucleic acid sequence encoding a linker region, wherein the linker region comprises a viral 2A peptide; and a second nucleic acid sequence encoding at least one gene of the multigene pathway.Type: ApplicationFiled: March 15, 2022Publication date: March 21, 2024Inventors: Daniel Close, Steven Ripp, Gary Sayler
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Publication number: 20210214732Abstract: The present disclosure relates to cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.Type: ApplicationFiled: March 23, 2021Publication date: July 15, 2021Inventors: Daniel Close, Steven Ripp, Gary Sayler, Michael Conway
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Patent number: 11046962Abstract: Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.Type: GrantFiled: May 29, 2020Date of Patent: June 29, 2021Inventors: Daniel Close, Steven Ripp, Gary Sayler, Michael Conway
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Publication number: 20200377896Abstract: Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.Type: ApplicationFiled: May 29, 2020Publication date: December 3, 2020Inventors: Daniel Close, Steven Ripp, Gary Sayler, Michael Conway
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Publication number: 20130031644Abstract: Disclosed are systems for expression of an autonomous lux reporter system in a vertebrate cell, such as mammalian or fish cell. In some examples the lux reporter system is operably connected to a pollutant-inducible DNA response element. Also disclosed are transgenic zebrafish, carrying pollution-inducible response elements, and methods of using such zebrafish to monitor pollutants.Type: ApplicationFiled: May 23, 2012Publication date: January 31, 2013Inventors: Steven A. Ripp, Gary S. Sayler, Daniel M. Close, Michelle Connolly, Theodore B. Henry
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Patent number: 7371538Abstract: An integrated microluminometer includes an integrated circuit chip having at least one n-well/p-substrate junction photodetector for converting light received into a photocurrent, and a detector on the chip for processing the photocurrent. A distributed electrode configuration including a plurality of spaced apart electrodes disposed on an active region of the photodetector is preferably used to raise efficiency.Type: GrantFiled: June 14, 2005Date of Patent: May 13, 2008Assignee: UT-Battelle, LLCInventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
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Patent number: 7300792Abstract: The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells.Type: GrantFiled: April 4, 2003Date of Patent: November 27, 2007Assignee: University of Tennessee Research FoundationInventors: Rakesh K. Gupta, Stacy S. Patterson, Gary S. Sayler, Steven A. Ripp
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Patent number: 7208286Abstract: Monolithic bioelectronic devices for the detection of ammonia includes a microorganism that metabolizes ammonia and which harbors a lux gene fused with a heterologous promoter gene stably incorporated into the chromosome of the microorganism and an Optical Application Specific Integrated Circuit (OASIC). The microorganism is generally a bacterium.Type: GrantFiled: June 14, 2005Date of Patent: April 24, 2007Assignee: UT-Battelle LLCInventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
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Publication number: 20070072174Abstract: A recombinant phage system has been developed for the rapid detection of bacteria, particularly fecal coliform indicator bacteria. The systems of the invention link phage infection events to quorum sensing signal molecule biosynthesis and bioluminescent bioreporter induction, facilitating the detection of pathogens that may be present in low numbers. The phage-based systems of the invention maintain specificity for the pathogen while still producing significant signal amplification for sensitive and quantitative detection. The systems require only the combination of sample with phage and bioreporter organisms; no extraneous addition of any substrates or user intervention of any kind is necessary, making this approach significantly less technical than standard molecular or immunological methods.Type: ApplicationFiled: September 28, 2005Publication date: March 29, 2007Inventors: Gary Sayler, Steven Ripp, Alice Layton
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Publication number: 20070003994Abstract: Monolithic bioelectronic devices for the detection of ammonia includes a microorganism that metabolizes ammonia and which harbors a lux gene fused with a heterologous promoter gene stably incorporated into the chromosome of the microorganism and an Optical Application Specific Integrated Circuit (OASIC). The microorganism is generally a bacterium.Type: ApplicationFiled: June 14, 2005Publication date: January 4, 2007Applicant: UT-BATTELLE, LLCInventors: Michael Simpson, Michael Paulus, Gary Sayler, Bruce Applegate, Steven Ripp
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Publication number: 20060281067Abstract: An integrated microluminometer includes an integrated circuit chip having at least one n-well/p-substrate junction photodetector for converting light received into a photocurrent, and a detector on the chip for processing the photocurrent. A distributed electrode configuration including a plurality of spaced apart electrodes disposed on an active region of the photodetector is preferably used to raise efficiency.Type: ApplicationFiled: June 14, 2005Publication date: December 14, 2006Applicant: UT-BATTELLE, LLCInventors: Michael Simpson, Michael Paulus, Gary Sayler, Bruce Applegate, Steven Ripp
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Patent number: 7090992Abstract: Bioelectronic devices for the detection of estrogen include a collection of eukaryotic cells which harbor a recombinant lux gene from a high temperature microorganism wherein the gene is operably linked with a heterologous promoter gene. A detectable light-emitting lux gene product is expressed in the presence of the estrogen and detected by the device.Type: GrantFiled: June 14, 2005Date of Patent: August 15, 2006Assignee: UT-Battelle, LLCInventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
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Publication number: 20060014228Abstract: Bioelectronic devices for the detection of estrogen inlcude a collection of eukaryotic cells which harbor a recombinant lux gene from a high temperature microorganism wherein the gene is operably linked with a heterologous promoter gene. A detectable light-emitting lux gene product is expressed in the presence of the estrogen and detected by the device.Type: ApplicationFiled: June 14, 2005Publication date: January 19, 2006Applicant: UT-BATTELLE, LLCInventors: Michael Simpson, Michael Paulus, Gary Sayler, Bruce Applegate, Steven Ripp
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Publication number: 20060008837Abstract: The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells.Type: ApplicationFiled: August 1, 2005Publication date: January 12, 2006Inventors: John Sanseverino, Alice Layton, Rakesh Gupta, Gary Sayler, Steven Ripp, Stacey Patterson
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Patent number: 6905834Abstract: Disclosed are monolithic bioelectronic devices comprising a bioreporter and an OASIC. These bioluminescent bioreporter integrated circuit are useful in detecting substances such as pollutants, explosives, and heavy-metals residing in inhospitable areas such as groundwater, industrial process vessels, and battlefields. Also disclosed are methods and apparatus for detection of particular analytes, including ammonia and estrogen compounds.Type: GrantFiled: September 12, 2000Date of Patent: June 14, 2005Assignee: UT-Battelle, LLCInventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
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Patent number: 6673596Abstract: Disclosed are bioluminescent bioreporter integrated circuit devices that detect selected analytes in fluids when implanted in the body of an animal. The device comprises a bioreporter that has been genetically engineered to contain a nucleic acid segment that comprises a cis-activating response element that is responsive to the selected substance operably linked to a gene encoding a bioluminescent reporter polypeptide. In preferred embodiments, the target analyte is glucose, glucagons, or insulin. Exposure of the bioreporter to the target substance causes the response element to up-regulate the nucleic acid sequence encoding the reporter polypeptide to produce a luminescent response that is detected and quantitated. In illustrative embodiments, the bioreporter device is encapsulated on an integrated circuit that is capable of detecting the emitted light, processing the resultant signal, and then remotely reporting the results.Type: GrantFiled: December 2, 1999Date of Patent: January 6, 2004Assignees: UT-Battelle, LLC, University of Tennessee Research CorporationInventors: Gary S. Sayler, Michael L. Simpson, Bruce M. Applegate, Steven A. Ripp
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Publication number: 20040002148Abstract: The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells.Type: ApplicationFiled: April 4, 2003Publication date: January 1, 2004Inventors: Rakesh K. Gupta, Stacy S. Patterson, Gary S. Sayler, Steven A. Ripp
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Publication number: 20030108980Abstract: The invention relates to devices and methods that utilize immobilized bacterial bioreporters genetically engineered to emit light visible to the naked eye in the presence of selected analytes. An exemplary bioreporter is an E. coli that has been modified to respond to mercury II as a result of incorporation of a merRop/lux gene cassette into its genome. Systems employing analagously engineered microorganisms can detect selected toxins quickly without need for expensive instruments or highly trained technicians.Type: ApplicationFiled: August 6, 2001Publication date: June 12, 2003Inventors: Gary S. Sayler, Steven A. Ripp, John Sanseverino
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Patent number: 6544729Abstract: Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.Type: GrantFiled: July 20, 2001Date of Patent: April 8, 2003Assignees: University of Tennessee, Purdue Research FoundationInventors: Gary S. Sayler, Steven A. Ripp, Bruce Applegate
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Publication number: 20030027241Abstract: Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.Type: ApplicationFiled: July 20, 2001Publication date: February 6, 2003Inventors: Gary S. Sayler, Steven A. Ripp, Bruce M. Applegate