Patents by Inventor Steven C. J. Geysens
Steven C. J. Geysens has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9359628Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an ?-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: GrantFiled: January 10, 2014Date of Patent: June 7, 2016Assignees: VIB, VZW, Research Corporation Technologies, Inc., Universiteit GentInventors: Roland Contreras, Nico L.M. Callewaert, Steven C.J. Geysens
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Patent number: 8883445Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for ?-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.Type: GrantFiled: July 13, 2007Date of Patent: November 11, 2014Assignees: Research Corporation Technologies, Inc., Universiteit Gent, Vib, VZWInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens, Vladimir Kaigorodov, Vervecken Wouter
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Publication number: 20140154738Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an ?-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: ApplicationFiled: January 10, 2014Publication date: June 5, 2014Applicants: VIB, VZW, UNIVERSITEIT GENT, RESEARCH CORPORATION TECHNOLOGIES, INC.Inventors: Roland Contreras, Nico L.M. Callewaert, Steven C.J. Geysens
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Patent number: 8663971Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an ?-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: GrantFiled: April 16, 2009Date of Patent: March 4, 2014Assignees: VIB, VZW, Research Corporation Technologies, Inc., Universiteit GentInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens
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Patent number: 8354268Abstract: The present invention provides genetically engineered strains of Pichiacapable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an ?-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: GrantFiled: September 25, 2003Date of Patent: January 15, 2013Assignees: VIB, VZW, Research Corporation Technologies, Inc., Universiteit GentInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens
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Publication number: 20100267084Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an ?-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: ApplicationFiled: April 16, 2009Publication date: October 21, 2010Applicants: VIB, VZW, RESEARCH CORPORATION TECHNOLOGIES, INC.Inventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens
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Patent number: 7252933Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for ?-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.Type: GrantFiled: June 26, 2002Date of Patent: August 7, 2007Assignee: Flanders Interuniversity Institute for BiotechnologyInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens, Vladimir Kaigorodov, Vervecken Wouter
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Patent number: 6803225Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an (&agr;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: GrantFiled: June 29, 2001Date of Patent: October 12, 2004Assignee: Flanders Interuniversity Institute for BiotechnologyInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens
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Publication number: 20040038381Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an &agr;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: ApplicationFiled: September 25, 2003Publication date: February 26, 2004Inventors: Roland Contreras, Nico L.M. Callewaert, Steven C.J. Geysens
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Publication number: 20040018588Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for &agr;-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.Type: ApplicationFiled: June 26, 2002Publication date: January 29, 2004Inventors: Roland Contreras, Nico L.M. Callewaert, Steven C. J. Geysens, Vladimir Kaigorodov, Vervecken Wouter
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Publication number: 20020188109Abstract: The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an (&agr;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.Type: ApplicationFiled: June 29, 2001Publication date: December 12, 2002Applicant: FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGYInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens