Abstract: In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Abstract: A method for patterning flow cell substrates using photo-initiated chemical reactions that includes fabricating a planar waveguide flow cell by forming a layer of light coupling gratings on a glass substrate layer; depositing a core layer on the layer of light coupling gratings; depositing a cladding layer on the core layer; and forming nanowells in the cladding layer; silanizing the cladding layer; coating the silanized cladding layer and nanowells with a first group of reactants; introducing a second group of reactants into the nanowells, wherein the second group of reactants includes a target reactant and a light-sensitive photoinitiator system; coupling a light source to the light coupling gratings and directing light internally within the planar waveguide flow cell for photo-initiating a chemical reaction between the first and second groups of reactants, wherein the photo-initiated chemical reaction covalently binds the target reactant to only the bottom portion of each nanowell.

Abstract: Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Abstract: Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Abstract: Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Abstract: A method for predicting a trait of interest in an agricultural sample comprises (a) obtaining a set of input data from: (i) at least one agronomic property; and (ii) at least one of a chemical property and physical property; (b) inputting the data into a processor containing at least one algorithm wherein the processor performs correlations of the input data with the trait of interest; and (c) outputting a predicted efficacy for the trait of interest. A computer-aided system comprises: (a) a computer readable medium including computer-executable instructions configured for estimating a trait of interest in an agricultural sample; (b) input data from: (i) at least one agronomic property; and (ii) at least one of a chemical property and physical property; and (c) an algorithm capable of correlating the data with the trait of interest; wherein the system outputs a predicted efficacy for the trait of interest. The trait of interest can include ethanol yield and/or digestibility.

Abstract: A method of predicting a trait of interest from a plant by measuring the degree of starch-protein association in the plant is provided. The degree of starch-protein association can be determined by identifying either chemical properties or physical properties, or both chemical and physical properties of plant cells. In a particular embodiment, the degree of starch-protein association is determined by identifying the protein composition of the plant.

Abstract: A method of determining whether an animal has ingested a plant of interest is provided. The method comprises screening the animal for the presence of at least one indicator of a plant of interest.