Patents by Inventor Stig Nielsson

Stig Nielsson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Selective inactivation of Aspergillus proteases
    Patent number: 6080564
    Abstract: A method is presented for inactivating undesired acid labile proteases which are expressed into the culture medium simultaneously with desired enzymes by Aspergillus species when Aspergillus species is used as the host for recombinantly producing enzymes. The method involves incubating culture medium which contains a mixture of the desired recombinantly produced enzyme and the undesired acid labile protease which have been expressed therein at pH values below 4.5 and at temperatures from 2.degree. C. to 75.degree. C. for at least 20 seconds.
    Type: Grant
    Filed: May 12, 1998
    Date of Patent: June 27, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Mads Aage Laustsen, Stig Nielsson
  • Crystallization of a protein with a sulphur salt
    Patent number: 6066481
    Abstract: The present invention relates to a method for crystallization of a protein obtained from a protein-containing solution which involves (a) treating the protein-containing solution with a salt containing a sulphur atom having an oxidation state less than 6, and (b) recovering the protein in crystalline form.
    Type: Grant
    Filed: August 27, 1998
    Date of Patent: May 23, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Stig Nielsson, Mads Aage Laustsen
  • Increased yields of a crystallized protein by using a solid adsorption material
    Patent number: 6031082
    Abstract: The invention deals with a method for crystallizing in increased yields a polypeptide or a protein obtained from a protein solution comprising more than one protein, comprising:(a) treating the protein solution with a solid adsorption material; and(b) crystallizing the polypeptide or the protein after said solid adsorption material has been removed; or(c) crystallizing the polypeptide or the protein without removing said adsorption material.
    Type: Grant
    Filed: August 27, 1998
    Date of Patent: February 29, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Stig Nielsson, Niels Murmann, Curran Simpson