Abstract: A solution phase assay of glycated proteins, such as glycated haemoglobin and plasma-soluble glycated proteins, is carried out by reacting the assay sample with a photoluminescent or chemiluminescent marker compound containing a boronic acid group which reacts selectively with the glycated protein. The luminescence of the marker compound is excited at a wavelength at which it is preferentially quenched by the binding of the marked compound to the glycated protein. The quenching in luminescence gives a measure of the degree of glycation of the glycated proteins.

Abstract: An immunogenic composition for raising antisera to a drug comprising immunologically active carrier protein to which is bound at least two types of hapten, each hapten comprising a drug molecule and, optionally, a bridging group, a method of raising antisera to a drug using said immunogenic composition, and a method of alleviating an overdose of a tricyclic depressant drug comprising an effective amount of antisera raised to an immunogen.