Abstract: The present invention provides an intelligent recruitment management system, method and computer program product for performing an automated recruitment process. The system comprises at least one processor (11) configured to execute modules of a recruitment intelligence platform (10), including a true requisition module (12) that parses data from intake forms and outputs a standardized result, analysing historical data of previous requisitions, a resume intelligence module (13) that mines resume and display a formatted output highlighting skills and best fit, cautioning frauds and plagiarism, a submission intelligence module (14) that enables candidates to submit relevant information via a unique link, generated by the true requisition module and a profile sourcing module (15) that invokes an RPA pipeline where a classification model automatically determines job matches and prompts the recruiters to select relevant candidates.

Abstract: The present invention provides an intelligent recruitment management system, method and computer program product for performing an automated recruitment process. The system comprises at least one processor (11) configured to execute modules of a recruitment intelligence platform (10), including a true requisition module (12) that parses data from intake forms and outputs a standardized result, analysing historical data of previous requisitions, a resume intelligence module (13) that mines resume and display a formatted output highlighting skills and best fit, cautioning frauds and plagiarism, a submission intelligence module (14) that enables candidates to submit relevant information via a unique link, generated by the true requisition module and a profile sourcing module (15) that invokes an RPA pipeline where a classification model automatically determines job matches and prompts the recruiters to select relevant candidates.

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

Abstract: Provided herein are polynucleotide conjugates comprising a polynucleotide and an agent, wherein the polynucleotide comprises a synthetic double stranded miR-29 mimic and the agent facilitates the delivery of the polynucleotide to a cell type or tissue type involved in fibrosis or inflammation. Also provided are methods of making, and uses thereof.

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract: The present invention provides nucleosides an exocyclic amine-protected nucleoside of the formula: A-B where A is a 5?-protected ribose, 5?-protected-2-deoxyribose, 5?-protected-3?-phosphoramidite ribose, or 5?-protected-3?-phosphoramidite-2-deoxyribose moiety; and B is a nucleobase having an exocyclic amine group that is protected with di-tert-butylisobutylsilyl (“BIBS”) protecting group. Use of BIBS protecting group provides an exocyclic amine-protected nucleoside that is stable to a wide variety of reaction conditions associated with oligonucleotide synthesis. The present invention also provides, oligonucleotides comprising the same, and methods for producing the same.

Abstract: The present invention provides a detection probe and a method for using and producing the same. The detection probe of the invention comprises boranephosphonate moiety and a target selective moiety. The present invention also provides a method for using the metal ion reducing properties of boranephosphonates (BPs) to determine the presence, the concentration or the location of a target molecule in a sample.

Abstract: The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract: The present invention provides nucleosides comprising a sterically hindered silyl protecting group on the exocyclic nitrogen atom of the nucleobase, oligonucleotides comprising the same, and methods for producing the same. In particular, the silyl protecting group is a sufficiently sterically hindered such that it is stable to a various oligonucleotide synthesis procedures.