Abstract: An improved gel formulation and method for high-voltage, short duration electrophoretic separation of isoforms of CK isoenzymes. The gel is an homogeneous mixture of agarose and a buffer having a molarity of about 0.05M. CK-MM and/or CK-MB isoforms are electrophoretically separated.

Abstract: An improved electrophoresis plate of the type including a base layer of an electrophoretic medium and opposed thickened ends made of an electrophoretic medium containing a desired fluid, such as a buffer material or solvent. The thickened ends function as a self-contained reservoirs during electrophoresis. The thickened ends of the present invention divert water generated by electroendosmosis or gel melt away from the electrophoresis area. This may be accomplished by providing a hollow region within the thickened ends which holds the water generated. Alternatively, serrations on the edges of the plate, or on the edges of the thickened ends of the plate which are disposed away from the electrophoresis area, may be provided to act as a path by which the water generated can flow away from the electrophoresis area.

Abstract: An electrophoresis plate of the type including a base layer of an electrophoretic medium containing some buffer material and a method of making such an electrophoresis plate. On the base layer of the plate there are formed buffer blocks made up of an electrophoretic medium having a lower electroendosmotic potential than that of the base layer. The buffer blocks contain buffer material for functioning as a self-contained reservoir during electrophoresis. The method of the present invention includes continuous casting of the buffer blocks and also includes casting the buffer blocks as a sequence of discrete layers.