Abstract: Fluidic device and methods of preparing a fibrous pad comprising dried reagents deposited thereon are described. In an embodiment, the fluidic devices comprise a fibrous pad; and a lyophilized reagent depot configured to support nucleic amplification of a target nucleic acid molecule to produce amplicons when dissolved, wherein the lyophilized reagent depot is disposed on the fibrous pad, the lyophilized reagent depot comprising: a nucleic acid amplification enzyme configured to perform a nucleic acid amplification reaction producing amplicons; and a lyophilization agent.

Abstract: Fluidic devices, systems, and methods for analyzing an analyte are described. In an embodiment, the fluidic devices include a housing defining a lysis chamber shaped to receive a biological sample; a lysis buffer storage chamber disposed within the housing and carrying a lysis buffer configured to lyse cells of the biological sample; a cap configured to cooperatively couple to the housing; a compressor configured to compress the lysis buffer storage chamber and expel the lysis buffer from the lysis buffer storage chamber and into the lysis chamber when the cap is uncoupled from the housing; and a porous membrane in selective fluidic communication with the lysis chamber.

Abstract: An apparatus, system, and method for filtering and assaying a fluid sample are described. In an embodiment, the apparatus includes a filtration unit comprising: a filter bracket shaped to removably couple with a fluid sample cup and a vacuum container; and a filter housing cooperatively couplable to the filter bracket and comprising a filter configured to filter fluid passing through the filter bracket; and an assay device shaped to cooperatively couple with the filter housing and comprising a porous matrix positioned to be in fluidic communication with the filter when the filter housing is cooperatively coupled with the assay device.

Abstract: Compositions and method for reducing the concentration of uremic toxins in the body of a patient suffering from some degree of kidney failure are disclosed. The methods can be used to delay the need for conventional dialysis treatment or as an adjunct therapy to reduce the frequency of dialysis sessions, and in some instances, as an alternative to such dialysis sessions.

Abstract: The present technology generally relates to stopped-flow microfluidic devices. Select embodiments of the present technology include microfluidic devices having a first porous element configured to receive a first fluid and a second porous element configured to receive a second fluid. The second porous element can have one or more legs overlapping with the first porous element. The device can be configured such that (a) delivery of the first fluid to the first porous element causes the first fluid to flow along the length of the first porous element without substantially wetting the one or more legs, and (b) delivery of the second fluid to the second porous element causes the second fluid to flow into the overlapping regions of the first porous element, thereby substantially stopping flow of the first fluid along at least a portion of the first porous element.

Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a porous receiving element having an input region and a receiving region, a first fluid source and a second fluid source positioned within the input region of the receiving element; wherein the first fluid source is positioned between the second fluid source and the receiving region, and wherein, when both the first and second fluid sources are in fluid connection with the input region, the device is configured to sequentially deliver the first fluid and the second fluid to the receiving region without leakage.

Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a porous receiving element having an input region and a receiving region, a first fluid source and a second fluid source positioned within the input region of the receiving element; wherein the first fluid source is positioned between the second fluid source and the receiving region, and wherein, when both the first and second fluid sources are in fluid connection with the input region, the device is configured to sequentially deliver the first fluid and the second fluid to the receiving region without leakage.

Abstract: The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, the system includes a vessel configured to receive a biological sample and a cap assembly that includes a porous membrane having a receiving region and a detection region. When the cap assembly is detachably coupled to an open end portion of the vessel, the system can be moved between a first orientation and a second orientation. When the system is in the first orientation, the biological sample is not in fluid communication with the receiving region. When the vessel contains is in the second orientation, the biological sample is in fluid communication with the receiving region and wicks through the porous membrane to the detection region.

Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a porous receiving element having an input region and a receiving region, a first fluid source and a second fluid source positioned within the input region of the receiving element; wherein the first fluid source is positioned between the second fluid source and the receiving region, and wherein, when both the first and second fluid sources are in fluid connection with the input region, the device is configured to sequentially deliver the first fluid and the second fluid to the receiving region without leakage.