Abstract: A method of obtaining one or more fractions from a plant material of Withania somnifera (WS) is disclosed. The method includes subjecting the plant material to hydro-alcoholic extraction in presence of a water-insoluble solvent to obtain at least one extract. The method further includes subjecting the at least one extract obtained from the hydro-alcoholic extraction to at least one of de-pigmentation, de-fatting and detoxification process to obtain the one or more fractions. The one or more fractions thus obtained contain Withaferin A in a concentration greater than concentrations of other pharmacologically active ingredients present in the one or more fractions. The one or more fraction thus obtained and one or more compositions containing the one or more fractions are effective in inhibiting proliferation of mammalian cancerous cells.

Abstract: A composition of matter comprising a synergistic formulation of plant extracts for hepatic and adrenal disorders is provided. In an implementation, an example extraction method for concentrating pharmacologically active fractions of plants provides a synergistically active compound containing approximately 5%-25% extract of Andrographis paniculata, approximately 10%-30% extract of Boerhavia diffusa, approximately 25%-50% extract of Phyllanthus niruri, and approximately 15%-40% extract of Tephrosia purpurea. The synergistically active compound provides greater therapeutic benefit than the individual ingredients alone, or the combined ingredients in different concentration ranges. The synergistic formulation may also be used for treatment of other medical conditions.

Abstract: A method of obtaining one or more fractions from a plant material of Withania somnifera (WS) is disclosed. The method includes subjecting the plant material to hydro-alcoholic extraction in presence of a water-insoluble solvent to obtain at least one extract. The method further includes subjecting the at least one extract obtained from the hydro-alcoholic extraction to at least one of de-pigmentation, de-fatting and detoxification process to obtain the one or more fractions. The one or more fractions thus obtained contain Withaferin A in a concentration greater than concentrations of other pharmacologically active ingredients present in the one or more fractions. The one or more fraction thus obtained and one or more compositions containing the one or more fractions are effective in inhibiting proliferation of mammalian cancerous cells.