Abstract: The regenerative sleeve encompasses the wound site of an amputated appendage and provides an environment conducive to tissue regeneration. The sleeve includes a tubular reservoir having an outer body that encloses the end of the appendage including the wound site and provides a sealed wound space between the wound site and the outer body. The sleeve also includes a cuff disposed in an opening formed in the outer body, the cuff being configured to fit on the appendage, and an access port disposed on the outer body and configured to allow administration of fluids to the wound space. The sleeve assembly was effective in supporting early stages of murine digit tip regeneration when combined with a porcine urinary bladder matrix (UBM) pepsin digest and electrical stimulation.