Abstract: A membrane having a stable low-contact angle that is used as a template in forming biological microarrays is provided. The membrane is formed of a polymeric material that has been surface modified by a first plasma treatment and subsequently by a second plasma treatment. The surface modification accomplished by the first plasma treatment results in a significant reduction in the contact angle for the membrane, causing the membrane to become hydrophilic, and the surface modification by the second membrane treatment permanently stabilizes the reduction in the contact angle produced by the first plasma treatment. The resulting membrane allows a solution containing a biological material to wet the surface of the membrane such that the membrane can quickly and easily form a biological microarray on substrate in which the features of the array are distinctly formed on the substrate.