Abstract: A compound microscope device allows simultaneous observation of one specimen by a transmission electron microscope and an optical microscope. The compound microscope device 1 of the present invention has a transmission electron microscope 2 and an optical microscope 4. A specimen 10 and a reflection mirror 41 are disposed on an electron optical axis C of an electron ray. The reflection mirror 41 is inclined from the electron optical axis C toward the optical object lens 43 and the specimen 10. Light from the specimen 10 (fluorescent light, reflection light, and the like) is reflected by the reflection mirror 41 and enters into the optical object lens 43. The electron ray from the electron microscope 2 passes through a mounting center hole 42 of the reflection mirror 41. This makes it possible to observe one specimen simultaneously by the electron microscope 2 and the optical microscope 4.

Abstract: Presentation of images showing with high resolution the state of a surgical field during surgery is realized with a simple configuration. When performing surgery, a three-dimensional model is generate based on MRI images of a patient captured prior to surgery, and three-dimensional coordinates of each location on a surface of the patient are measured by scanning a laser beam over the surface and detecting laser beam reflected therefrom, and correspondence is made with the MRI image for each location of the surface (frameless/markerless: steps 100 to 116), the position of a surgical instrument is also detected by the laser beam, the position of the surgical instrument derived, and images of the surgical instrument combined onto the MRI images and displayed during surgery (steps 120 to 130).

Abstract: A compound microscope device allowing simultaneous observation of one specimen by a transmission electron microscope and an optical microscope, is provided. A compound microscope device 1 of the present invention has a transmission electron microscope 2 and an optical microscope 4. A specimen 10 and a reflection mirror 41 are disposed on an electron optical axis C of an electron ray. The reflection mirror 41 is inclined from the electron optical axis C toward the optical object lens 43 and the specimen 10. Light from the specimen 10 (fluorescent light, reflection light, and the like) is reflected by the reflection mirror 41 and entered into the optical object lens 43. The electron ray from the electron microscope 2 passes through a mounting center hole 42 of the reflection mirror 41. This makes it possible to observe one specimen simultaneously by the electron microscope 2 and the optical microscope 4.

Abstract: The observing apparatus is being equipped with an image-forming optical system which forms an image of light emitted from a specimen, an imaging unit which picks up the image of the specimen formed by the image-forming optical system, and an illuminating unit which illuminates the specimen with a surface illuminant in which bright areas and dark areas are arranged alternately in order to provide an observing apparatus suitable to observe a transparent specimen with a wide field of view. If the position of the surface illuminant and the pitch of contrasting are set properly, each partial area of the specimen is illuminated obliquely at a small angle by each bright area of the surface illuminant. Therefore, the imaging unit can acquire a dark-field observation image of each partial area.

Abstract: Surgical operation supporting apparatus and method is disclosed in which based on a high-definition tomographic images of an operation site produced before surgery, a three-dimensional model of the operation site is generated, and a surface of the operation site is optically measured during the surgical operation, and further, first position information that represents a three-dimensional position of each of points on the surface is acquired. An unexposed portion of the operation site is measured with ultrasonic waves during the surgical operation, and second position information that represents a three-dimensional position of each of points in the unexposed portion is acquired. Based on the first position information and the second position information, displacement and distortion at a portion whose three-dimensional position is not known in the three-dimensional model is estimated to obtain an estimated result.

Abstract: To provide a cell sorting method and a cell sorter which are capable of performing sorting focusing on various changes in each cell as stress on living cells is kept as low as possible, and proliferating the sorted cells. The present invention performs cell sorting using a fiber unit in which a plurality of fibers is bundled so as to be separable and unitable. In the present invention, a cell group containing target cells targeted for sorting is dispersed in a fluid into which the fiber unit is immersed, target fibers to which the target cells are attached among the plurality of fibers, are separated from the fiber unit, and the target cells are proliferated by use of the separated target fibers.

Abstract: A device for displaying assistance information for surgical operation 1 includes an endoscope 11, a CT device 30, an image pickup device 20, a surface shape calculating unit 43, a coordinate axis matching unit 44 that matches the shape of the patient surface by the CT device 30 and the shape of the patient surface calculated from the image, an endoscope optical axis calculating unit 45 that calculates a ray indicative of an optical axis in the image pickup direction of the endoscope 11, an intersection calculating unit 46 that calculates an intersection of the ray and the plane constituting the internal parts of the patient 60, and an outputting unit 47 that outputs the information indicative of the intersection after overlapping the information on the information indicative of the shape of the patient 60.

Abstract: A cell sorting apparatus includes an extracorporeal circulation system 10 for sampling blood from a subject and circulating the blood outside a body; a cell measuring section 20 for supplying measurement light to cells contained in the blood to optically measure cell information on the physical quantity relating to the volume of the cell itself or a nucleus of the cell; and a cell separating section 30 for separating cells with reference to the cell information measured. The cell separating section 30 separates cells, based on the cell information measured by the cell measuring section 20 and a sorting condition set for sorting a specific type of cell such as a cancer cell, the cells satisfying the sorting condition, and returns the other cells into the body via the extracorporeal circulation system 10. Thus, a cell sorting apparatus can be realized which is capable of suitably sorting a target cell from the cells in blood.

Abstract: An evanescent catheter system having a tip portion of an optical fiber capable of securely measuring fluorescence intensity or fluorescence spectrum of a substance around a catheter tip, without being hindered by optical absorption of hemoglobin in erythrocytes in blood, and being capable of calculating an existing amount of a fluorescence-emitting substance existing in an evanescent light generating part. A protecting layer 22 and a cladding 23 are removed from an upper semicircular part of a core 24 at a tip of a catheter 21 to expose the core 24. When excitation light 29 is incident, evanescent light 25 is generated on the upper semicircular part of the core 24 and a fluorescent substance generates emits fluorescence 30. An interference filter 26 filters out the excitation light and only the fluorescence 30 reaches the photodiode 28, permitting a measuring device to measure an existing amount of the fluorescent substance.

Abstract: Presentation of images showing with high resolution the state of a surgical field during surgery is realized with a simple configuration. When performing surgery, a three-dimensional model is generate based on MRI images of a patient captured prior to surgery, and three-dimensional coordinates of each location on a surface of the patient are measured by scanning a laser beam over the surface and detecting laser beam reflected therefrom, and correspondence is made with the MRI image for each location of the surface (frameless/markerless: steps 100 to 116), the position of a surgical instrument is also detected by the laser beam, the position of the surgical instrument derived, and images of the surgical instrument combined onto the MRI images and displayed during surgery (steps 120 to 130).

Abstract: An object of the present invention is to accurately display to which region of a patient, a part the image of which is being picked up by an endoscope corresponds without the need to newly use a special endoscope.

Abstract: To provide a cell sorting method and a cell sorter which are capable of performing sorting focusing on various changes in each cell as stress on living cells is kept as low as possible, and proliferating the sorted cells. The present invention performs cell sorting using a fiber unit in which a plurality of fibers is bundled so as to be separable and unitable. In the present invention, a cell group containing target cells targeted for sorting is dispersed in a fluid into which the fiber unit is immersed, target fibers to which the target cells are attached among the plurality of fibers, are separated from the fiber unit, and the target cells are proliferated by use of the separated target fibers.

Abstract: The observing apparatus is being equipped with an image-forming optical system which forms an image of light emitted from a specimen, an imaging unit which picks up the image of the specimen formed by the image-forming optical system, and an illuminating unit which illuminates the specimen with a surface illuminant in which bright areas and dark areas are arranged alternately in order to provide an observing apparatus suitable to observe a transparent specimen with a wide field of view. If the position of the surface illuminant and the pitch of contrasting are set properly, each partial area of the specimen is illuminated obliquely at a small angle by each bright area of the surface illuminant. Therefore, the imaging unit can acquire a dark-field observation image of each partial area.

Abstract: A cell sorting apparatus includes an extracorporeal circulation system 10 for sampling blood from a subject and circulating the blood outside a body; a cell measuring section 20 for supplying measurement light to cells contained in the blood to optically measure cell information on the physical quantity relating to the volume of the cell itself or a nucleus of the cell; and a cell separating section 30 for separating cells with reference to the cell information measured. The cell separating section 30 separates cells, based on the cell information measured by the cell measuring section 20 and a sorting condition set for sorting a specific type of cell such as a cancer cell, the cells satisfying the sorting condition, and returns the other cells into the body via the extracorporeal circulation system 10. Thus, a cell sorting apparatus can be realized which is capable of suitably sorting a target cell from the cells in blood.

Abstract: An evanescent catheter system is provided as a system having a structure of a tip portion of an optical fiber capable of securely measuring a fluorescence intensity or fluorescence spectrum of a substance around a catheter tip, without being hindered by optical absorption of hemoglobin in erythrocytes in blood, and being capable of calculating an existing amount of a fluorescence-emitting substance existing in an evanescent light generating part. A protecting layer 22 and a cladding 23 are removed from an upper semicircular part of a core 24 at a tip of a catheter 21 to expose the core 24. An optical filter 26 and a photodiode 28 are located opposite to a lower semicircular part. When excitation light 29 is incident, evanescent light 25 is generated on the upper semicircular part of the core 24 and a fluorescent substance generates emits fluorescence 30.

Abstract: A switch-type imaging fiber apparatus and a branch-type imaging fiber apparatus are provided. Light emitted from a laser light source is made incident on an opening of an IF (image fiber) guided by a GM (galvanometer), output from an optical system provided at a leading end portion of the IF, and applied to an observation target. The light reflected by the observation target is sent back to the IF, transformed into parallel light by an f? objective lens, reflected by a dichroic mirror, made incident on an image pickup device (CCD) via an imaging lens, and can be observed on a monitor. The GM is controlled to switch to make the light incident on the opening of an arbitrary one of a plurality of IFs. Accordingly, observation can be performed by focusing the light solely on the arbitrary IF.

Abstract: Surgical operation supporting apparatus and method is disclosed in which based on a plurality of high-definition tomographic images of an operation site produced before surgery, a three-dimensional model of the operation site is generated, and a surface of the operation site is optically measured during the surgical operation, and further, first position information that represents a three-dimensional position of each of points on the surface of the operation site is acquired. Further, an unexposed portion of the operation site is measured with ultrasonic waves during the surgical operation, and the second position information that represents a three-dimensional position of each of points in the unexposed portion of the operation site is acquired. Moreover, based on the first position information and the second position information, displacement and distortion occurring at each of the points in the operation site are estimated using the generated three-dimensional model.

Abstract: A drill unit equipped with a camera, a body of which has a camera hole at the center, and two holes formed to the left and right side of the camera hole 4 including a discharge hole 2 for discharging air and water to the drilled portion, and a suction hole 3 for sucking water and air from the drilled portion. A first and a second drill holes are formed at the top and bottom of the camera hole. Illuminating holes are also formed to illuminate the drilled portion. The camera serves to project an image of the drilled portion. The first and the second drilling members are used to fracture the body tissues, to clean the fractured portion with water, and to suck water used for the cleaning so as to be discharged. The body of the drill unit is rotated to change the drilling position for the fracture in the same way for forming the hole led to the affected portion.

Abstract: A switch-type imaging fiber apparatus and a branch-type imaging fiber apparatus are provided. Light emitted from a laser light source is made incident on an opening of an IF (image fiber) guided by a GM (galvanometer), output from an optical system provided at a leading end portion of the IF, and applied to an observation target. The light reflected by the observation target is sent back to the IF, transformed into parallel light by an f? objective lens, reflected by a dichroic mirror, made incident on an image pickup device (CCD) via an imaging lens, and can be observed on a monitor. The GM is controlled to switch to make the light incident on the opening of an arbitrary one of a plurality of IFs. Accordingly, observation can be performed by focusing the light solely on the arbitrary IF.