Abstract: An apparatus and methods for using biological material to discriminate an agent. In one embodiment of the present invention, the method includes the steps of providing at least one cell, exposing at least one cell to an agent, measuring the response of the cell to the agent in terms of a physical quantity related to at least one of the cellular physiological activities of the cell, and identifying the agent from the measured response. The method further includes the step of quantifying the agent from the measured response.

Abstract: An apparatus and methods for detecting at least one analyte of interest either produced or consumed by a plurality of cell. In one embodiment of the present invention, the method includes the steps of providing a housing defining a chamber, placing a plurality of cells in the chamber, and simultaneously detecting at least two analytes of interest either produced or consumed by the plurality of cells in the chamber.

Abstract: An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.

Abstract: An apparatus and methods for monitoring the status of a cell that consumes oxygen. In one embodiment of the present invention, the method includes the steps of confining the cell in a sensing volume, measuring dynamically intracellular or extracellular signaling of the cell, and determining the status of the cell from the measured intracellular or extracellular signaling of the cell.

Abstract: An apparatus and methods for using biological material to discriminate an agent. In one embodiment of the present invention, the method includes the steps of providing at least one cell, exposing at least one cell to an agent, measuring the response of the cell to the agent in terms of a physical quantity related to at least one of the cellular physiological activities of the cell, and identifying the agent from the measured response. The method further includes the step of quantifying the agent from the measured response.

Abstract: An apparatus and methods for detecting at least one analyte of interest either produced or consumed by a plurality of cell. In one embodiment of the present invention, the method includes the steps of providing a housing defining a chamber, placing a plurality of cells in the chamber, and simultaneously detecting at least two analytes of interest either produced or consumed by the plurality of cells in the chamber.

Abstract: A cutting tool, preferably for milling, includes a holder and a cutting insert mounted thereon. The holder includes a seat upon which the insert is mounted, and an upstanding wall structure against which an edge face of the insert is pressed. The edge face has upper and lower portions separated by a step. The upper portion is frustoconical and is pressed against an upper wall of the wall structure to frictionally resist rotation of the insert about the insert's center axis. The lower portion of the edge face is polygonal and is adapted to engage a lower wall of the wall structure at a location spaced radially inwardly from a location where the upper portion and upper wall make contact. The lower portion rotates into engagement with the lower wall in response to cutting forces acting on the insert, to create an interference relationship between the lower portion and lower wall which prevents further rotation of the insert.

Abstract: An end mill includes a shank, a pull rod having a first end connected to a front end of the shank, and a second end connected to an end mill head. The end mill head has a centering surface engageable with a centering surface formed on either the pull rod or shank. The end mill head includes a radially extending abutment surface engageable with a radial abutment surface formed on the shank.

Abstract: An improved metal electrode for the determination of pH, P.sub.O.sbsb.2 or P.sub.CO.sbsb.2 in liquids is disclosed. The improved electrode consists of a metal sensor (e.g., antimony) in a holder characterized in that the metal is monocrystalline with only one plane crystal face exposed to the liquid.