Patents by Inventor Sven Hermsmeier
Sven Hermsmeier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11661595Abstract: Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: GrantFiled: March 17, 2020Date of Patent: May 30, 2023Assignee: Lonza Cologne GmbHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20220298466Abstract: The invention relates to a method and device for controlling a filling level of a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles within at least one chamber of a device for applying an electric field to the suspension. In order to avoid overfilling of the chamber if multiple electroporation cycles are performed and to achieve exact filling in an environment of unpredictable chamber volume, the amount of suspension filled into the chamber is dynamically limited in the course of several electroporation cycles by determining at least one change of the electrical resistance at the outlet port. The resistance between at least one electrode and a grounding electrode is measured during the filling procedure of each cycle at several points in time. Once a change of resistance is detected, the termination routine is initiated and the filling procedure is finally terminated.Type: ApplicationFiled: June 28, 2020Publication date: September 22, 2022Inventors: Sven HERMSMEIER, Timo GLEISSNER
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Patent number: 11352615Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: GrantFiled: November 9, 2018Date of Patent: June 7, 2022Assignee: Lonza Cologne GmbHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20200325466Abstract: Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: ApplicationFiled: March 17, 2020Publication date: October 15, 2020Applicant: Lonza Cologne GmbHInventors: Ludger ALTROGGE, Timo GLEISSNER, Andreas HEINZE, Sven HERMSMEIER
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Patent number: 10633646Abstract: The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: GrantFiled: April 28, 2015Date of Patent: April 28, 2020Assignee: LONZA COLOGNE GMBHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Patent number: 10336996Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: GrantFiled: April 28, 2015Date of Patent: July 2, 2019Assignee: LONZA COLOGNE GMBHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20190144844Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: ApplicationFiled: November 9, 2018Publication date: May 16, 2019Applicant: Lonza Cologne GmbHInventors: Ludger ALTROGGE, Timo GLEISSNER, Andreas HEINZE, Sven HERMSMEIER
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Publication number: 20170233715Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: ApplicationFiled: April 28, 2015Publication date: August 17, 2017Inventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20170233716Abstract: The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: ApplicationFiled: April 28, 2015Publication date: August 17, 2017Inventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier