Patents by Inventor Sylvie Darche

Sylvie Darche has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for determining the quantity of a DNA fragment of interest by a method of enzymatic amplification of DNA
    Patent number: 5747246
    Abstract: The present invention relates to a process for determining the quantity of a DNA fragment of interest in a sample wherein:1) a standard DNA fragment can be amplified with the same oligonucleotide primers is added to the sample to be analyzed containing the DNA fragment of interest, the standard DNA fragment and the fragment of interest differing in sequence and/or in size by not more than 10%,2) the DNA fragment of interest and the standard fragment are coamplified with the same primers, preferably to saturation of the amplification of the DNA fragment of interest,3) one or more labeled oligonucleotide primer(s), specific for the DNA fragment of interest and the standard fragment and different from the primers of step 2), is/are added to the reaction medium obtained in step 2), so that, after denaturation of the DNA, said primer(s) hybridize(s) with said fragments at a suitable site in order that an elongation with the DNA polymerase generates labeled DNA fragments of different sizes and/or sequences or with
    Type: Grant
    Filed: June 21, 1994
    Date of Patent: May 5, 1998
    Assignees: Institute National de la Sante et de la Recherche Medicale (INSERM), Institut Pasteur
    Inventors: Christophe Pannetier, Madeleine Cochet, Sylvie Darche, Philippe Kourilsky