Abstract: Methods, compositions, and systems for fermentation, particularly large scale operations for production of ethanol and dried distiller's grain are provided. Addition of a transglutaminase source to a process stream aids in removing protein from the system in order to provide a high protein feed product and/or provide clarified backset for fermentation. Transglutaminase can be in reagent form or can be produced by microorganisms added to the process stream.

Abstract: The present disclosure includes methods of making a fertilizer composition from pretreated biomass by removing one or more inorganic nutrients from a fermentation beer to form an aqueous fertilizer composition. The present disclosure also includes systems configured to make a fertilizer composition from pretreated biomass, where the systems include one or more apparatuses in fluid communication with a fermentation vessel, wherein at least one apparatus is configured to remove one or more inorganic nutrients from the fermentation beer to form an aqueous fertilizer composition.

Abstract: The present disclosure includes methods of making a fertilizer composition from pretreated biomass by removing one or more inorganic nutrients from a fermentation beer to form an aqueous fertilizer composition. The present disclosure also includes systems configured to make a fertilizer composition from pretreated biomass, where the systems include one or more apparatuses in fluid communication with a fermentation vessel, wherein at least one apparatus is configured to remove one or more inorganic nutrients from the fermentation beer to form an aqueous fertilizer composition.