Abstract: The present invention relates to a novel hyperthermophile, in particular, to a hyperthermophile belonging to a novel genus isolated from compost by fermenting organic wastes at a temperature of 85° C. or more which is capable of growing at 80° C. or more (Caldothrix satsumae). The hyperthermophile of the present invention does not multiply at 50° C. or below, but grows and multiplies vigorously at 70 to 85° C. It is non-spore forming, gram-negative, and an absolutely aerobic rod. The hyperthermophile is useful as a fermenting microbe when organic wastes are fermented at high temperatures to produce compost. Further the hyperthermopile can be utilized for the production of heat resistant enzymes.

Abstract: The present invention relates to a novel hyperthermophile, in particular, to a hyperthermophile belonging to a novel genus isolated from compost by fermenting organic wastes at a temperature of 85° C. or more which is capable of growing at 80° C. or more (Caldothrix satsumae). The hyperthermophile of the present invention does not multiply at 50° C. or below, but grows and multiplies vigorously at 70 to 85° C. It is non-spore forming, gram-negative, and an absolutely aerobic rod. The hyperthermophile is useful as a fermenting microbe when organic wastes are fermented at high temperatures to produce compost. Further the hyperthermopile can be utilized for the production of heat resistant enzymes.

Abstract: The invention relates to an ammonia elimination reagent for use in an enzymatic assay of a biological substance which produces ammonia as the reaction product. The reagent is an alkaline solution of pH 9 to 11 and contains Sulfolobus-derived thermostable isocitrate dehydrogenase, 2-oxoglutaric acid, a reducing coenzyme, isocitric acid, glutamate dehydrogenase, and a divalent metal salt. The reagent can be stored in solution and has excellent stability under conditions of alkaline pHs.

Abstract: A DNA polymerase from Thermus thermophilus which retains at least 60% of its activity when placed at pH 8.0 and 85.degree. C. for 2 hours is disclosed. The DNA polymerase has a pH optimum of about 8.0, a temperature optimum of about 75.degree. C., a mass of about 85 to 95 kDa and an optimal saline concentration of about 20-60 mM NaCl.

Abstract: A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.

Abstract: A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.