Abstract: The coat protein of Mirafiori lettuce virus was purified from highly purified Mirafiori lettuce virus, and its partial amino acid sequences were determined. DNA encoding the coat protein of Mirafiori lettuce virus was cloned by the polymerase chain reaction using primers designed based on the determined amino acid sequence information, and its primary structure was elucidated. Moreover, the present inventors succeeded in isolating a number of DNA molecules encoding coat protein of Mirafiori lettuce virus by carrying out 5? RACE using primers designed based on the obtained sequence information, and in determining the primary structure. The use of these DNA molecules and their primary structures enables the production of plants resistant to Mirafiori lettuce virus, and the diagnosis of Mirafiori lettuce virus infections.

Abstract: The coat protein of lettuce big-vein virus (LBVV) was purified from highly purified LBVV, and its partial amino acid sequences were determined. An RNA encoding the coat protein of LBVV was cloned by polymerase chain reaction using primers designed based on the determined amino acid sequences information, and its primary structure was elucidated. Moreover, the present inventors succeeded not only in isolating RNA molecules of a plurality of LBVV-encoded proteins, including LBVV polymerase, by carrying out 3?RACE and 5?RACE using primers designed based on the resulting sequence information, but also in determining their primary structure. It was found that the use of these made it possible to produce lettuce resistant to LBVV and to diagnose infections with LBVV.

Abstract: The coat protein of lettuce big-vein virus (LBVV) was purified from highly purified LBVV, and its partial amino acid sequences were determined. An RNA encoding the coat protein of LBVV was cloned by polymerase chain reaction using primers designed based on the determined amino acid sequences information, and its primary structure was elucidated. Moreover, the present inventors succeeded not only in isolating RNA molecules of a plurality of LBVV-encoded proteins, including LBVV polymerase, by carrying out 3?RACE and 5?RACE using primers designed based on the resulting sequence information, but also in determining their primary structure. It was found that the use of these made it possible to produce lettuce resistant to LBVV and to diagnose infections with LBVV.

Abstract: The coat protein of Mirafiori lettuce virus was purified from highly purified Mirafiori lettuce virus, and its partial amino acid sequences were determined. DNA encoding the coat protein of Mirafiori lettuce virus was cloned by the polymerase chain reaction using primers designed based on the determined amino acid sequence information, and its primary structure was elucidated. Moreover, the present inventors succeeded in isolating a number of DNA molecules encoding coat protein of Mirafiori lettuce virus by carrying out 5? RACE using primers designed based on the obtained sequence information, and in determining the primary structure. The use of these DNA molecules and their primary structures enables the production of plants resistant to Mirafiori lettuce virus, and the diagnosis of Mirafiori lettuce virus infections.

Abstract: The coat protein of lettuce big-vein virus (LBVV) was purified from highly purified LBVV, and its partial amino acid sequences were determined. An RNA encoding the coat protein of LBVV was cloned by polymerase chain reaction using primers designed based on the determined amino acid sequences information, and its primary structure was elucidated. Moreover, the present inventors succeeded not only in isolating RNA molecules of a plurality of LBVV-encoded proteins by carrying out 3′RACE and 5′RACE using primers designed based on the resulting sequence information, but also in determining their primary structure. Similarly, an RNA encoding polymerase of LBVV was also successfully isolated, and its primary structure was determined. It was found that the use of these made it possible to produce lettuce resistant to LBVV and to diagnose infections with LBVV.