Abstract: A fluorescence detection apparatus comprising: a sample holder for fixing and holding sample vessels on a circular arc; a partition plate being joined to drive means for rotation on the center of the circular arc; an excitation light source; excitation light optical means; and fluorescence optical means containing a light guide being fixed on the partition plate for rotation integrally and a photosensor. The photosensor is mechanically discontinued from the drive means and fixedly placed. The fluorescence signal emission end of the light guide is placed facing the photosensor on the rotation center axis and the partition plate and the parts fixed thereto are rotated integrally, whereby fluorescence detection of the samples arranged on the circular arc is repeated in order.

Abstract: A simple, speedy and sensitive method of detecting HIV-RNA using an oligonucleotide which can bind to an intramolecularly free region of the genomic RNA of HIV-1 at relatively low and constant temperatures (at 35° C. to 50° C., preferably at 41° C.) as an oligonucleotide primer for use in amplification of a nucleic acid.

Abstract: An oligonucleotide capable of binding to the intramolecular structure-free region of Verotoxin type 1 RNA or Verotoxin type 2 RNA at relatively low and constant temperature, and which can be used in a constant temperature nucleic acid amplification method, is provided. Also, a simple, speedy and highly sensitive method for detecting Verotoxin type 1 RNA or Verotoxin type 2 RNA is provided.

Abstract: A compound represented by the formula 1, a salt, hydrate, solvate or stereoisomer thereof.

Abstract: A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.

Abstract: An oligonucleotide useful for specific amplification of HCV RNA, or highly sensitive detection and identification of HCV RNA, and a combination thereof. A detection method using RNA amplification step, wherein the oligonucleotide of SEQ ID NO: 11 is used as a first primer and the oligonucleotide of SEQ ID NO: 6 or 7 as a second primer, the oligonucleotide of SEQ ID NO: 12 is used as a first primer and the oligonucleotide of SEQ ID NO: 7 as a second primer, or the oligonucleotide of SEQ ID NO: 13 is used as a first primer and the oligonucleotide of SEQ ID NO: 9 as a second primer.

Abstract: Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.

Abstract: An oligonucleotide for cleavage, detection or amplification of the mecA gene, a gene element of methicillin-resistant Staphylococcus aureus (MRSA), or RNA derived from said gene is provided. Further, a method for detecting the mecA gene is provided.

Abstract: A fluorescence detection apparatus is provided which comprises a sample holder for holding stationarily sample vessels deployed along a circle line or concentric circle lines having different radiuses, a partition plate connected to a driving means to be rotatable around the center of the circle line or concentric circle lines, an optical means for excitation light and an optical means for fluorescence light fixed respectively to the partition plate to be rotatable in integration therewith, a first light guide constituted of numerous optical fibers, a photosensor, and a light source for generating the excitation light, wherein the partition plate, the optical means for excitation light, and the optical means for fluorescence are integrally rotated, and thereby the fluorescence of the sample arranged along the circle line is successively detected and the detected fluorescence is transmitted to the photosensor.

Abstract: An apparatus including a sample holder which fixedly holds sample vessels arranged on a same arc, a partition plate, a light guide which is configured by optical fibers and which transmits fluorescent signals emitted from respective test samples, to an optical sensor, the single optical sensor, and a light source which generates excitation light. Fluorescent signal emission ends of the light guide are opposed to the optical sensor, and fluorescent signal incidence ends of the light guide are respectively opposed to the sample vessels via the partition plate therebetween. The partition plate includes an excitation light optical unit for selectively guiding the excitation light from the light source to only one of the sample vessels arranged on the arc, and a fluorescence optical unit for guiding only the fluorescent signal emitted from a selected one of the sample vessels to the light guide.

Abstract: Oligonucleotides, for detection of small round structured virus (SRSV) RNA, which bind specifically to SRSV RNA at a relatively low, constant temperature (for example, 41° C.), and an SRSV RNA detection method, which is a constant temperature nucleic acid amplification method employing the oligonucleotides, are provided.

Abstract: A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and

Abstract: An oligonucleotide for detection or amplification of a gene selected from the group consisting of Vibrio parahaemolyticus thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and Vibrio parahaemolyticus thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.

Abstract: A method of specifically cleaving a double-stranded DNA (a target nucleic acid) at a specific nucleic acid sequence, which comprises irradiating a solution containing at least the target nucleic acid, a nucleic acid probe (a single-stranded oligonucleotide) linked to an intercalater and spermine with light with an absorption wavelength of the intercalater.

Abstract: A DNA probe of the following structural formula (1) (wherein P* is an optically active phosphorus atom, each of R1 and R2 is a DNA oligomer having an arbitrary sequence, and R3 is a fluorescent intercalative dye attached via an appropriate linker) which has an optically active configuration about P*.

Abstract: A method of assay of a specific nucleic acid anticipated in a sample, which comprises:a DNA producing step which involves production of a double-stranded DNA having a promoter sequence for an RNA polymerase and the nucleic sequence of the specific nucleic acid (the specific nucleic acid sequence) downstream from the promoter sequence by using the specific nucleic acid in the sample as a template, andan RNA producing and measuring step which involves production of a single-stranded RNA having the specific nucleic acid sequence by the RNA polymerase and measurement of the single-stranded RNA,wherein the RNA producing and measuring step is initiated by adding at least the RNA polymerase, ribonucleoside triphosphates and a probe which is labeled with a fluorescent intercalative dye and is complementary to the single-stranded RNA to the reaction solution after the DNA producing step, involves measurement of the fluorescence intensity of the reaction solution, and is carried out at a constant temperature, and does

Abstract: A method of measuring a melting temperature of a nucleic acid, which comprises a step of monitoring the fluorescent intensity of a mixture of a sample and a probe which is labeled with a fluorescent intercalative dye and contains a base sequence complementary to a specific nucleic acid in the sample, while varying the temperature of the mixture.

Abstract: A labeled nucleic acid probe comprising a single-stranded oligonucleotide probe having a nucleic acid sequence complementary to a specific nucleic acid sequence in a target nucleic acid and an intercalating fluorochrome bound to said probe is used to detect the target nucleic acid by a convenient, single-stage method in a homogeneous system. The formation of a complementary bond between the probe and the target nucleic acid can be detected and the amount of the resulting complementary binding product determined without requiring any extra step such as for removing the excess probe which has not participated in the complementary binding.

Abstract: A thermal cycling reaction apparatus comprises a reactor having a reactor body made of a thin heat-conductive plate having a cavity as a reaction chamber with an opening of the chamber sealed with a transparent heat-resistant sheet. The apparatus further includes, delivery rollers for delivering the reactor along a delivery path and stopping it at stopping positions in a predetermined order; plural temperature-controlling blocks having respectively a temperature-controlling surface to be brought into contact with a heat-transfer face of the reactor and being placed at the stopping positions separately so as not to thermally affect each other; and a temperature-controlling mechanism for keeping the fixed temperature-controlling surfaces respectively at prescribed temperatures.

Abstract: An oxidizing composition in the form of powder for oxidizing a chemiluminescent substance, including an oxidizing agent and a binder.