Patents by Inventor Takahito Ohshiro
Takahito Ohshiro has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230194472Abstract: The present disclosure provides a method for analyzing a microRNA using a tunneling current. The present disclosure provides a method for identifying the base sequence and/or modification state of a microRNA using a tunneling current, and a system and a program to be used in the method. Furthermore, the present disclosure provides a method for analyzing the conditions of a subject, said method comprising determining the base sequence and/or modification state of a microRNA using a tunneling current. For example, methylation modification can be analyzed thereby.Type: ApplicationFiled: April 24, 2020Publication date: June 22, 2023Inventors: Masateru Taniguchi, Hideshi Ishii, Takahito Ohshiro, Masamitsu Konno
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Publication number: 20220283110Abstract: Provided is a device that facilitates a sample to enter a sample measurement channel in which measuring electrodes are arranged. A device used in measurement of tunnel current includes: a base material; a channel formed in the base material; and a pair of measuring electrodes for measuring tunnel current occurring when a sample passes between the pair of measuring electrodes. The channel includes a sample supply channel, a sample measurement channel in which the measuring electrodes are arranged, a first taper channel arranged between the sample supply channel and the sample measurement channel and having a channel width that decreases from the sample supply channel to the sample measurement channel, and a sample collection channel used for collecting a sample that passed through the sample measurement channel. The width of a connection part between the first taper channel and the sample measurement channel is 20 nm to 200 nm.Type: ApplicationFiled: March 3, 2022Publication date: September 8, 2022Inventors: Takahito OHSHIRO, Masateru TANIGUCHI, Yuki KOMOTO
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Publication number: 20220187244Abstract: A current measurement method for measuring a tunneling current in biopolymers passing through between a pair of electrodes includes arranging the electrodes in a liquid that contains an electrolyte and, while applying a voltage between the electrodes, measuring a current flowing between the electrodes via an electric double layer formed along surfaces of the electrodes. This enables measuring the current in consideration of the electric double layer. As a result, it is possible to more accurately measure the tunneling current in the biopolymers included in a liquid sample that contains an electrolyte.Type: ApplicationFiled: February 20, 2020Publication date: June 16, 2022Inventors: Masateru TANIGUCHI, Takahito OHSHIRO, Masakazu SANADA, Tadashi MIYAGI
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Publication number: 20210232870Abstract: A PU classification device includes a classifier that performs maximum likelihood classification of an instance to be classified as a positive instance or a negative instance based on a magnitude relationship between a first probability that the instance is sampled from a population distribution for learning as the positive instance and a second probability that the instance is sampled from the population distribution for learning, when the instance to be classified is given, and a processor that learns the classifier by estimating a distribution function of the first probability from a set of positive instances sampled from the population distribution for learning and by estimating a distribution function of the second probability from a set of instances that are sampled from the population distribution for learning and are unknown whether they are positive or negative, wherein an instance to be classified is classified as the positive instance or the negative instance by using the classifier learned by theType: ApplicationFiled: March 28, 2019Publication date: July 29, 2021Inventors: Takashi Washio, Masateru Taniguchi, Takahito Ohshiro, Takeshi Yoshida
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Publication number: 20210140938Abstract: The present invention provides an identification method by which appropriately identifies nonconforming data from a measurement data set, for example, contributes to improve the reliability of measurement results by the advanced sensing device, a classification analysis method which can perform the classification analysis with high accuracy for the measurement data, an identification device, a classification analysis device, a storage medium for identification and a storage medium for classification analysis. A feature value is obtained in advance which indicates the feature of waveform of pulse signal, and the feature value obtained is set as the learning data for machine learning.Type: ApplicationFiled: April 9, 2018Publication date: May 13, 2021Applicant: AIPORE INC.Inventors: Takashi WASHIO, Masateru TANIGUCHI, Takahito OHSHIRO, Takeshi YOSHIDA, Takayuki TAKAAI
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Patent number: 10557167Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.Type: GrantFiled: March 2, 2017Date of Patent: February 11, 2020Assignee: QUANTUM BIOSYSTEMS INC.Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
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Publication number: 20190242846Abstract: Methods and systems are provided for creation of stable and consistent nanoelectrode pairs for detection of biomolecules, such as deoxyribonucleic acid.Type: ApplicationFiled: February 4, 2019Publication date: August 8, 2019Inventors: Masateru TANIGUCHI, Takahito Ohshiro, Makusu Tsutsui, Kazumichi Yokota, Toshiki Matsuoka, Mark Oldham, Eric Nordman
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Publication number: 20180023132Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.Type: ApplicationFiled: March 2, 2017Publication date: January 25, 2018Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
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Publication number: 20170306396Abstract: The nano-gap electrode pair 12 is disposed so that a biomolecule joined to at least one or more types of a single molecule included in a sample passes an opposing position, and the strength of the electric field in a position spaced only a predetermined distance on the downstream side from the opposing position 64 becomes stronger than the strength of the electric field in a position spaced only the predetermined distance on the upstream side from the opposing position 64.Type: ApplicationFiled: August 19, 2016Publication date: October 26, 2017Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro
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Publication number: 20170146511Abstract: The present disclosure provides devices, systems and methods for effectuating nanoelectrodes for use with determining the sequence of double stranded biopolymers. Various modified bases and different metals may be utilized alone or in combination so as to provide differentiation between different nucleobases and to determine which base is associated with which strand.Type: ApplicationFiled: November 4, 2016Publication date: May 25, 2017Inventors: Masateru Taniguchi, Tomoji Kawai, Takahito Ohshiro
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Patent number: 9644236Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.Type: GrantFiled: March 4, 2016Date of Patent: May 9, 2017Assignee: QUANTUM BIOSYSTEMS INC.Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
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Patent number: 9535033Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.Type: GrantFiled: August 2, 2013Date of Patent: January 3, 2017Assignee: QUANTUM BIOSYSTEMS INC.Inventors: Tomoji Kawai, Takahito Ohshiro, Masateru Taniguchi
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Publication number: 20160377591Abstract: The present disclosure provides a biomolecule sequencing device that includes at least one set of nano-gap electrodes arranged so that a current flows when a biomolecule contained in a sample passes in proximity to the set of nano-gap electrodes, an electrophoresis electrode pair for forming an electric field for moving the biomolecule between the electrodes of the set of nano-gap electrodes, a flow path for flowing the sample in a direction towards the nano-gap electrode pair, a flow path for flowing the sample in a direction away from the nano-gap electrode pair, a measurement unit configured to measure a tunnel current generated when the biomolecule passes between the electrodes of the nano-gap electrode pair with an electric field being formed, and an identification unit configured to sequence the biomolecule.Type: ApplicationFiled: July 22, 2016Publication date: December 29, 2016Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Floyd Oldham, Eric Scott Nordman
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Publication number: 20160319342Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.Type: ApplicationFiled: March 4, 2016Publication date: November 3, 2016Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
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Publication number: 20150219593Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.Type: ApplicationFiled: August 2, 2013Publication date: August 6, 2015Inventors: Tomoji Kawai, Takahito Ohshiro, Masateru Taniguchi
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Publication number: 20140055150Abstract: A maximum current value and pulse continuation duration are measured for each of plural pulses of tunnel current arising as a polynucleotide passes through between an electrode pair, and the polynucleotide base sequence is determined based on the maximum current value and the pulse continuation duration.Type: ApplicationFiled: March 29, 2013Publication date: February 27, 2014Applicant: OSAKA UNIVERSITYInventors: Tomoji Kawai, Takahito Ohshiro, Kazuki Matsubara, Masayuki Furuhashi, Makusu Tsutsui, Masateru Taniguchi
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Patent number: 8124417Abstract: A method is herein presented for analyzing nucleobases on a single molecular basis (a single molecule detection), which comprises scanning a molecular tip chemically modified with the complementary nucleobase on the nucleobases, and measuring the tunneling currents between the scanned nucleobases and the molecular tips with scanning tunneling microscopy.Type: GrantFiled: December 28, 2005Date of Patent: February 28, 2012Assignee: Japan Science and Technology AgencyInventors: Yoshio Umezawa, Takahito Ohshiro
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Publication number: 20090155917Abstract: A method is herein presented for analyzing nucleobases on a single molecular basis (a single molecule detection), which comprises scanning a molecular tip chemically modified with the complementary nucleobase on the nucleobases, and measuring the tunneling currents between the scanned nucleobases and the molecular tips with scanning tunneling microscopy.Type: ApplicationFiled: December 28, 2005Publication date: June 18, 2009Inventors: Yoshio Umezawa, Takahito Ohshiro