Patents by Inventor Takamichi Miyazaki

Takamichi Miyazaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cell culture method using laminin fragment-containing medium
    Patent number: 11505785
    Abstract: Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.
    Type: Grant
    Filed: November 8, 2016
    Date of Patent: November 22, 2022
    Assignee: Kyoto University
    Inventors: Hirofumi Suemori, Takamichi Miyazaki
  • CELL CULTURE METHOD USING LAMININ FRAGMENT-CONTAINING MEDIUM
    Publication number: 20180334656
    Abstract: Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.
    Type: Application
    Filed: November 8, 2016
    Publication date: November 22, 2018
    Inventors: Hirofumi Suemori, Takamichi Miyazaki
  • Culture substrate for human pluripotent stem cells and use thereof
    Patent number: 8877493
    Abstract: The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin ?5?1?1 E8 fragment or human laminin ?3?3?2 E8 fragment preferably at a concentration of 0.5 to 25 ?g/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.
    Type: Grant
    Filed: October 7, 2010
    Date of Patent: November 4, 2014
    Assignees: Osaka University, Kyoto University
    Inventors: Kiyotoshi Sekiguchi, Sugiko Futaki, Yukimasa Taniguchi, Maria Hayashi, Norio Nakatsuji, Takamichi Miyazaki, Eihachiro Kawase, Hirofumi Suemori
  • CULTURE SUBSTRATE FOR HUMAN PLURIPOTENT STEM CELLS AND USE THEREOF
    Publication number: 20120220031
    Abstract: The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin ?5?1?1 E8 fragment or human laminin ?3?3?2 E8 fragment preferably at a concentration of 0.5 to 25 ?g/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.
    Type: Application
    Filed: October 7, 2010
    Publication date: August 30, 2012
    Inventors: Kiyotoshi Sekiguchi, Sugiko Futaki, Yukimasa Taniguchi, Maria Hayashi, Norio Nakatsuji, Takamichi Miyazaki, Eihachiro Kawase, Hirofumi Suemori