Abstract: The present invention relates to a modified (mutant) nicotinamide phosphoribosyltransferase, and a method for producing nicotinamide mononucleotide using the same. The modified nicotinamide phosphoribosyltransferase (Nampt) of the present invention comprises the amino acid sequence represented by the following SEQ ID NO: 1 and/or the amino acid sequence represented by the following SEQ ID NO: 2, and has improved activity as compared with wild-type Nampt: (a) SEQ ID NO: 1: S- [V/I] -P-A-X1-X2-H-S-[T/V/I] - [M/V/I] -X3, and (b) SEQ ID NO: 2: X4-[S/I] -D-X5 wherein X1 to X5 are as defined herein.

Abstract: A canister includes a casing defining an adsorbent chamber. The casing includes a tank port in fluid communication with a fuel tank and an atmospheric port in fluid communication with the atmosphere. The canister includes at least three adsorbent sections arranged in series in the adsorbent chamber. The at least three adsorbent sections include a first adsorbent section proximate to the atmospheric port, a second adsorbent section disposed on a tank port side of the first adsorbent section, and a third adsorbent section disposed on a tank port side of the second adsorbent section. The first adsorbent section contains a first adsorbent, the second adsorbent section contains a second adsorbent, and the third adsorbent section contains a third adsorbent. An adsorption capacity of the first adsorbent is equal to or greater than an adsorption capacity of the second adsorbent. The adsorption capacity of the second adsorbent is greater than an adsorption capacity of the third adsorbent.

Abstract: An evaporated fuel treatment apparatus includes: a canister; a purge passage; a purge pump provided in the purge passage; and an atmosphere passage communicating with the canister. A leakage diagnostic device includes: two bypass passages provided between the purge passage on the upstream side of the purge pump and the atmosphere passage; a reference orifice; two three-way valves; a pressure sensor; and an ECU. When operating the purge pump under a predetermined condition, the ECU controls the two three-way valves to set a mode in which atmospheric air is pressure-fed into an air intake passage through the reference orifice and the purge pump, and a mode in which vapors in the canister are pressure-fed into the air intake passage through the reference orifice and the purge pump, and determines leakage by comparing a reference pressure and a leakage pressure that are measured when these modes are set.

Abstract: A canister includes a casing defining an adsorbent chamber. The casing includes a tank port in fluid communication with a fuel tank and an atmospheric port in fluid communication with the atmosphere. The canister includes at least three adsorbent sections arranged in series in the adsorbent chamber. The at least three adsorbent sections include a first adsorbent section proximate to the atmospheric port, a second adsorbent section disposed on a tank port side of the first adsorbent section, and a third adsorbent section disposed on a tank port side of the second adsorbent section. The first adsorbent section contains a first adsorbent, the second adsorbent section contains a second adsorbent, and the third adsorbent section contains a third adsorbent. An adsorption capacity of the first adsorbent is equal to or greater than an adsorption capacity of the second adsorbent. The adsorption capacity of the second adsorbent is greater than an adsorption capacity of the third adsorbent.

Abstract: An object of the present invention is to provide a method for producing nicotinamide mononucleotide (NMN) with excellent production efficiency. The method for producing NMN according to the present invention (the first aspect) comprises the step of bringing a transformant with enhanced expression of enzymes nicotinamide phosphoribosyltransferase (Nampt), phosphoribosyl pyrophosphate synthetase (Prs) and polyphosphate kinase (Ppk), a cell-free protein synthesis reaction solution having the three enzymes expressed, or a treated product thereof into contact with a mixture containing ribose-5-phosphate (R5P), nicotinamide (NAM), ATP and polyphosphate.

Abstract: A fuel vapor processing apparatus form an internal combustion engine provided with a turbocharger includes a canister and an ejector. The canister is configured to adsorb fuel vapor from a fuel tank. The ejector is configured to generate a negative pressure by supercharged air flowing from an intake passage on a downstream side of the turbocharger to the intake passage on an upstream side of the turbocharger, so that fuel vapor in the canister is purged by the negative pressure. The ejector includes an ejector housing extending in a discharge direction of the supercharged air. The ejector housing is welded to a tubular member defining a passage wall of the intake passage such that the supercharged air is discharged into the intake passage on the upstream side of the turbocharger and that the discharge direction of the supercharged air is parallel to a direction of flow of intake air.

Abstract: An evaporated fuel treatment apparatus includes a canister for collecting vapor, a vapor passage for introducing the vapor from a fuel tank to the canister, a purge passage for introducing the vapor from the canister to an intake passage, an atmosphere passage for introducing atmospheric air into the canister, a purge pump provided in the atmosphere passage to pressure feed the vapor to the intake passage, a bypass atmosphere passage branching from the atmosphere passage downstream of the purge pump, an atmosphere check valve for opening/closing the atmosphere passage between a branch portion of the bypass passage and the purge pump, and an atmosphere opening/closing valve for opening/closing the bypass atmosphere passage. During operation of the purge pump, the atmosphere check valve is opened and the atmosphere opening/closing valve is closed. During non-operation of the purge pump, the atmosphere check valve is closed and the atmosphere opening/closing valve is opened.

Abstract: A fuel vapor processing apparatus form an internal combustion engine provided with a turbocharger includes a canister and an ejector. The canister is configured to adsorb fuel vapor from a fuel tank. The ejector is configured to generate a negative pressure by supercharged air flowing from an intake passage on a downstream side of the turbocharger to the intake passage on an upstream side of the turbocharger, so that fuel vapor in the canister is purged by the negative pressure. The ejector includes an ejector housing extending in a discharge direction of the supercharged air. The ejector housing is welded to a tubular member defining a passage wall of the intake passage such that the supercharged air is discharged into the intake passage on the upstream side of the turbocharger and that the discharge direction of the supercharged air is parallel to a direction of flow of intake air.

Abstract: Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (X1 to X27 represent independent arbitrarily-defined amino acid residuals). (a) X1 is valine or glycine (b) X9 is valine or threonine (c) X23 is an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) X24 is leucine.

Abstract: Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (X1 to X27 represent independent arbitrarily-defined amino acid residuals). (a) X1 is valine or glycine (b) X9 is valine or threonine (c) X23 is an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) X24 is leucine.

Abstract: A vaporized fuel treatment device has formed therein a passage (3) through which fluid can flow. The passage (3) comprises a primary chamber (21) which is provided with a primary adsorption layer (11) and a secondary chamber (22) which is located on the atmosphere port (6) side of the primary chamber (21). The secondary chamber (22) has a first adsorption layer (12), a second adsorption layer (13), and a third adsorption layer (14), which are provided serially from the primary adsorption layer (11) side. The secondary chamber (22) also has separation sections (31, 32) for separating the adjacent adsorption layers. With respect to the volume of the primary adsorption layer (11), the volume of the first adsorption layer (12) is set in the range of 4.0% to 8.5%, inclusive, the volume of the second adsorption layer (13) is set in the range of 1.2% to 3.0%, inclusive, and the volume of the third adsorption layer (14) is set in the range of 0.9% to 2.2%, inclusive.

Abstract: An evaporation fuel processing device is provide including: a passage; a tank port and a purge port on one end side of the passage; an atmospheric air port on the other end side of the passage; and adsorbent layers filled with adsorbent for evaporation fuel components, provided in the passage; a region provided on an atmospheric air port side of the passage, being constituted of three or more adsorbent layers and separating parts for separating the adjacent adsorbent layers, in which a volume of the adsorbent layer is smaller in the adsorbent layer closer to the atmospheric air port, a volume of the separating part is larger closer to the atmospheric air port, and the volume of the separating part located farthest on a tank port side is larger than that of the adsorbent layer located farthest on the atmospheric air port side.

Abstract: The present invention provides an evaporation fuel processing device including: a passage formed inside so as to allow a fluid to flow through the passage; a tank port and a purge port formed on one end side of the passage; an atmospheric air port formed on the other end side of the passage; and adsorbent layers filled with adsorbent which can adsorb evaporation fuel components, the adsorbent layers being provided in the passage, wherein a region which is constituted of three or more adsorbent layers and separating parts for separating the adjacent adsorbent layers, and in which a total volume of the adsorbent layers is set smaller than a total volume of the separating parts, is provided on an atmospheric air port side of the passage.

Abstract: A fuel vapor processing apparatus may include a first canister and a second canister each having an adsorbent disposed therein. The second canister may include a first passage and a second passage each communicating between the first canister and an atmosphere. The first passage may be configured as an adsorption passage having the adsorbent disposed therein. The second passage may be configured as an air passage through which air flows. A control device may control flow of the air through the air passage. A heating device may heat the air flowing through the air passage. The air passage may be arranged so that the adsorbent of the adsorption chamber is heated by heat of the air that is heated by the heating device and flows through the air passage.

Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.

Abstract: The present invention includes a fuel vapor processing apparatus including a communication passage connecting between a first space and a second space each containing an adsorption material capable of adsorbing fuel vapor. The communication passage includes a dissipation delaying space capable of delaying dissipation or transmission of the fuel vapor between the first and second spaces. The communication passage further includes throttles positioned to oppose to each other across the dissipation delaying space.

Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.

Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.

Abstract: A fuel vapor processing apparatus may include a first canister and a second canister each having an adsorbent disposed therein. The second canister may include a first passage and a second passage each communicating between the first canister and an atmosphere. The first passage may be configured as an adsorption passage having the adsorbent disposed therein. The second passage may be configured as an air passage through which air flows. A control device may control flow of the air through the air passage. A heating device may heat the air flowing through the air passage. The air passage may be arranged so that the adsorbent of the adsorption chamber is heated by heat of the air that is heated by the heating device and flows through the air passage.

Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.