Publication number: 20150353944
Abstract: A method for transforming a stramenopile includes transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. The foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (?5 desaturase gene, ?12 desaturase gene and/or ?3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
Type:
Application
Filed:
August 21, 2015
Publication date:
December 10, 2015
Applicants:
KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION, UNIVERSITY OF MIYAZAKI, KONAN GAKUEN, NIPPON SUISAN KAISHA, LTD.
Inventors:
Keishi Sakaguchi, Takanori Matsuda, Takumi Kobayashi, Makoto Ito, Naoki Nagano, Masahiro Hayashi, Daisuke Honda, Yosuke Taoka, Yuji Okita, Hitoshi Izumida, Shinichi Sugimoto
Patent number: 9150891
Abstract: Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (?5 desaturase gene, ?12 desaturase gene and/or ?3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
Type:
Grant
Filed:
September 24, 2010
Date of Patent:
October 6, 2015
Assignees:
KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, UNIVERSITY OF MIYAZAKI, KONAN GAKUEN, NIPPON SUISAN KAISHA, LTD.
Inventors:
Keishi Sakaguchi, Takanori Matsuda, Takumi Kobayashi, Makoto Ito, Naoki Nagano, Masahiro Hayashi, Daisuke Honda, Yosuke Taoka, Yuji Okita, Hitoshi Izumida, Shinichi Sugimoto