Abstract: A nucleic acid sequence measurement method for measuring a target RNA having a specific nucleic acid sequence included in a cell suspension by hybridization, including a step of heating the cell suspension at 100° C. to 200° C. in a pressurized state to obtain an RNA extract, a step of adding a proteolytic enzyme to the RNA extract to cause the RNA extract to be reacted, thereby preparing a sample solution, a step of supplying the sample solution to a device for nucleic acid sequence measurement, the device being equipped with a fluorescent probe that hybridizes with the target RNA, a step of subjecting the target RNA and the fluorescent probe to a hybridization reaction, and a step of measuring fluorescence from the device for nucleic acid sequence measurement.

Abstract: A method for producing a complex, a method for determining microbial inclusion, and a method for identifying the included microorganism, which can be easily performed is provided. A method for producing a complex containing a target that is contained in a sample, includes a step (a) of bringing a sample into contact with a label molecule A, a label molecule B, and a label molecule C and forming a complex, and a step (b) of isolating the complex. The target is a nucleic acid. The label molecule A contains a nucleic acid sequence capable of hybridizing to a part of the target and substantially complementary to the nucleic acid sequence of the target. The label molecule B contains a nucleic acid sequence capable of hybridizing to a part of the target and substantially complementary to the nucleic acid sequence of the target.

Abstract: A target (50) having a specific nucleic acid sequence included in a sample is measured using a detection probe (10) that has a detection part having a nucleic acid sequence complementary to the nucleic acid sequence of the target (50), a label molecule A (20) having a nucleic acid sequence that is complementary to the nucleic acid sequence of the target (50) and different from the nucleic acid sequence of the detection part, and a label molecule B (30) having a nucleic acid sequence complementary to the nucleic acid sequence of the label molecule A (20), in which a fluorescent molecule is attached to either one of the label molecule A (20) and the label molecule B (30), and the label molecule A (20) and the label molecule B (30) bind to each other at at least two or more sites to form a branched structural body of the label molecule A (20) and the label molecule B (30).

Abstract: A target measurement method for measuring a target contained in a sample, includes, when a solution containing a light-absorbing substance that absorbs excitation light that excites a fluorescent molecule or fluorescence emitted from the fluorescent molecule is in contact with a solid-phase surface of a substrate that is provided with a bound product of the target and a capture molecule specifically binding to the target which is modified with a fluorescent molecule, measuring a fluorescence obtained by irradiation of the excitation light from an opposite side to the solid-phase surface of the substrate from the opposite side to the solid-phase surface of the substrate.

Abstract: A nucleic acid sequence measuring apparatus (1) measures a target (TG) having a specific nucleic acid sequence included in a sample.

Abstract: When the target (30) is not supplied, the binding via the binding part is maintained, and when the donor fluorescent molecule (11) is excited, energy is transferred to the acceptor fluorescent molecule (21) that is close to the donor fluorescent molecule (11). Then, the acceptor fluorescent molecule (21) exhibits fluorescence. When the target (30) is supplied, the target (30) is bound to the detection part to release the binding via the binding part, and the acceptor fluorescent molecule (21) is separated from the donor fluorescent molecule (11). Then, the donor fluorescent molecule (11) exhibits fluorescence.

Abstract: In a case where the target (30) is not supplied, binding through a nucleic acid sequence from the base end on a side of the solid phase surface of the complementary sequence complementary to a part of the nucleic acid sequence of the detection sequence to the other base end is maintained, which causes fluorescence of the fluorescent molecule (11) to be quenched by the quenching molecule (21) close to the fluorescent molecule (11). In a case where the target (30) is supplied, the target is bound to the detection sequence and the binding through the complementary sequence is released, which causes the fluorescent molecule (11) to separate from the quenching molecule (21) and emit fluorescence.

Abstract: A nucleic acid sequence measuring method includes measuring fluorescence from the nucleic acid sequence measuring device supplied with a sample solution. The device includes a fluorescent probe added with a fluorescent molecule, and a quenching probe added with a quenching substance. The fluorescent probe and/or the quenching probe has a detection part detecting a predetermined nucleic acid sequence. Fluorescence from the fluorescent molecule is quenched by the quenching substance coupled with the fluorescent molecule when the hybridization between the detection target nucleic acid and the detection part has not occurred, and fluorescence is emitted from the fluorescent molecule separated from the quenching substance when the hybridization has occurred.

Abstract: A nucleic acid sequence measuring method includes measuring fluorescence from the nucleic acid sequence measuring device supplied with a sample solution. The device includes a fluorescent probe added with a fluorescent molecule, and a quenching probe added with a quenching substance. The fluorescent probe and/or the quenching probe has a detection part detecting a particular nucleic acid sequence. Fluorescence from the fluorescent molecule is quenched by the quenching substance coupled with the fluorescent molecule when the hybridization between the detection target nucleic acid and the detection part has not occurred, and fluorescence is emitted from the fluorescent molecule separated from the quenching substance when the hybridization has occurred.