Patents by Inventor Takehiro Sagara
Takehiro Sagara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230160023Abstract: Provided is a method for detecting an FNA virus in a biological sample, the method comprising: (1) a step for preparing a sample solution containing a biological sample, a protease, a nucleic acid that does not become a target of nucleic acid amplification, and at least one additive selected from the group consisting of chaotropic reagents and surfactants; (2) a step for preparing a nucleic acid amplification reaction solution containing the sample solution prepared in step (1) and containing a polypeptide having reverse transcriptase activity and DMA polymerase activity or a polypeptide having reverse transcription activity and a polypeptide having DNA polymerase activity; and (3) a step for amplifying a nucleic acid of the RNA virus in the reaction solution prepared in step (2).Type: ApplicationFiled: April 28, 2021Publication date: May 25, 2023Applicant: TAKARA BIO INC.Inventors: Takashi UEMORI, Takehiro SAGARA, Miwa AKITOMO, Kensuke SAITO
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Patent number: 10975415Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.Type: GrantFiled: September 9, 2015Date of Patent: April 13, 2021Assignees: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUENInventors: Takashi Uemori, Yoshizumi Ishino, Takehiro Sagara, Sonoko Ishino, Takeshi Yamagami, Tsuyoshi Shirai
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Publication number: 20180163263Abstract: Provided are: a method with which it is possible to suppress nucleic-acid amplification of non-target nucleic acids and to selectively nucleic-acid amplify a target nucleic acid in a sample in which a large amount of non-target nucleic acids and a rare target nucleic acid are present in mixture; a method for detecting at high sensitivity rare mutant genes, the method combining this nucleic-acid amplification method and a specific real-time method for detecting the target nucleic acid; and a composition and kit for this method.Type: ApplicationFiled: March 18, 2016Publication date: June 14, 2018Applicant: TAKARA BIO INC.Inventors: Takashi UEMORI, Takehiro SAGARA, Koichi INOUE
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Publication number: 20170253909Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.Type: ApplicationFiled: September 9, 2015Publication date: September 7, 2017Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUENInventors: Takashi UEMORI, Yoshizumi ISHINO, Takehiro SAGARA, Sonoko ISHINO, Takeshi YAMAGAMI, Tsuyoshi SHIRAI
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Patent number: 9447388Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: GrantFiled: July 12, 2012Date of Patent: September 20, 2016Assignees: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.Inventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Publication number: 20140322793Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: ApplicationFiled: July 12, 2012Publication date: October 30, 2014Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Publication number: 20080206811Abstract: A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.Type: ApplicationFiled: August 19, 2005Publication date: August 28, 2008Applicant: Takara Bio Inc.Inventors: Toshihiro Shodai, Hiroshi Kobori, Takehiro Sagara, Hiroshi Endo, Hikaru Takakura, Jun Tomono, Hiroaki Sagawa, Hiroyuki Mukai, Ikunoshin Kato